Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30673
Investigation the Difference of Several Hormones Correlated to Reproduction between Infertile and Fertile Dairy Cows

Authors: Ali M. Mutlag, Yang Zhiqiang, Meng Jiaren, Zhang Jingyan, Li Jianxi

Abstract:

The object of this study was to investigate several hormones correlated to the reproduction and inhibin A, inhibin B and NO levels in the infertile dairy cows as attempt to illustrate the physiological causes of dairy cows infertility.

40 Holstein cow (21 infertile and 19 fertile) were used at estrous phase of the cycle, Hormones FSH, LH, E2, Testosterone, were measured using ELISA method. inhibin A and B also estimated by ELISA method, Nitric oxide was measured by Greiss reagent method.

The results showed different concentrations of the hormone in which FSH illustrated significantly higher concentration in the infertile cows than fertile cows (P<0.05). LH and E2 showed significant decrease in the infertile cows than the fertile cows (P<0.05), no significant difference appeared in testosterone concentrations in the fertile cows and infertile cows (P>0.05). The both inhibins A and B showed significant P<0.05 decrease concentrations in the infertile cows also NO showed clearly significant decrease P<0.05 in the infertile cows.

In conclusion, the present study approved the poorly ovarian activities and reproduction disturbance of infertile cows in spite of trigger estrous signs, the study confirmed a positive correlation between inhibins and NO to regulate the ovarian physiology. These inhibins represent effective markers of dairy cow infertility.

Keywords: infertility, cows, nitric oxide (NO), Inhibin (A

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1089271

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1551

References:


[1] Plaizier J.C., Lissemore K.D., Kelton D., King G.J. (1998). Evaluation of overall reproductive performance of dairy herds. J. Dairy. Sci.; 81:1848-54.
[2] Roche J.F., Mackey D., and Diskin M.D. (2000). Reproductive management of postpartum cows. Anim. Reprod. Sci.; 60:703-12.
[3] Lucy M.C. (2001). Reproductive loss in high-producing dairy cattle: where will it end. J Dairy Sci., 84: 1277 – 93.
[4] Guo K. (2004). Effects of milk urea nitrogen and other factors on probability of conception of dairy cows. M.Sc. thesis. Faculty of the Graduate School of the University of Maryland.
[5] Al-Dahash S.Y.A., and Bensassi M.F., (2009). Treatment of some infertility problems in cows using Dalmarelin and Dalmazin. Iq. J. Vet. Sci.; 23, (II), 255-257.
[6] Peng X., Cao B., Deng G.Z., Li C.Y., Ye L.L., and Yu H. (2011). Echography characteristics of abnormal ovaries in infertile dairy cows. J. Anim. Vet. Advan. 10 (9): 1166 – 70.
[7] Itoh M., Kondo M., Kojima C., Jin W., Watanabe G., Taya K., Motoharu H.M., and Shimizu K., (2003). Inhibin B is the major form of inhibin secreted from testes in male Japanese macaques (Macaca fuscata). Primates; 44:253–257.
[8] Ling N, Ying S.Y., Ueno N., Esch F., Denoroy L., and Guillemin R. (1985). Isolation and partial characterization of a Mr 32,000 protein with inhibin activity from porcine follicular fluid. P.N.A.S; 82:7217–21.
[9] Natsuko N.N., Nambo Y., Nagata S., Nagaoka K., Tsunoda N., Taniyama H., Tanaka Y., Tohei A., Watanabe G., and Taya K. (1998). Inhibin Secretion in the Mare: Localization of Inhibina, bA, and bB Subunits in the Ovary. Bio. Rep., 59, 1392–1398.
[10] Moncada S, Palmer R.M.J., and Higgs E.A. (1991). Nitric oxide: physiology, patho-physiology and pharmacology. Phamaco. Rev.; 43:109 –142
[11] Najafi T., Novin M.G., Pakravesh J., Foghi K., Fadayi F., and Rahimi G. (2011). Immunohistochemical localization of endothelial nitric oxide synthase in endometrial tissue of women with unexplained infertility. In. J. Reprod. Med. 10(2): 121-126.
[12] Zhang X., Lin H.Y., Liu G.Y., Wang H.M., Li Q.L., and Zhu C. (2005). Expressions and regulation of endothelial and inducible nitric oxide synthases in mouse uterus during the estrous cycle and early pregnancy. Fron. Bio., 10: 3172–3182.
[13] Baylis C., Beinder E., Suto T., and August P. (1998). Recent insights into the roles of nitric oxide and renin-angiotensin in the pathophysiology of preeclamptic pregnancy. Seminars in Nephrology. 18:208–230.
[14] Pecsok S.R., McGilliard M.L., and Nebel R.L. (1994). Conception rates. 1. Derivation and estimates for effects of estrus detection on cow profitability. J. Dairy. Sci., 77:3008-15.
[15] Danilovich N., Babu P.S., Xing W., Gerdes M., Krishnamurthy H., and Sairam M.R. (2000). Estrogen deficiency, obesity and skeletal abnormalities in follicle-stimulating hormone receptor knockout (FORKO) female mice. Endo; 141:4295–4308.
[16] Picton H., Briggs D., and Gosden R. (1998). The molecular basis of oocyte development. Mol. Cel. Endo. 145:27–37.
[17] Wolfenson D, Inbar G., Roth Z., Kaim M., Bloch A., and Braw-Tal R. (2004). Follicular dynamics and concentrations of steroids and gonadotropins in lactating cows and nulliparous heifers. Therio.; 15:1042-55.
[18] Taya K., Kaneko H., Takedomi T., Kishi H., and Watanabe G. (1996). Role of inhibin in regulation of FSH secretion and folliculogenesis in cows. Anim. Repro. Sci.; 42:563–570.
[19] Garrett W.M., Mack SO., Rohan RM., and Guthrie HD., (2000). In situ analysis of the changes in expression of ovarian inhibin subunit mRNAs during follicle recruitment after ovulation in pigs. J of Rep and Fer; 118, 235–242.
[20] Danilovich N., Javeshghani J., Xing W., and Sairam M. R. (2002). Endocrine Alterations and Signaling Changes Associated with Declining Ovarian Function and Advanced Biological Aging in Follicle-Stimulating Hormone Receptor Haploinsufficient Mice. Bio of Rep; 67,370–378.
[21] Rettori V., Belova N., Dees W.L., Nyberg C.L., Gimeno M., and Mccann S.M. (1993). Role of nitric oxide in the control of luteinizing hormone-releasing hormone release in vivo and in vitro. Proc. Natl. Acad. Sci.; 90: 10130-10134.
[22] Tessaro I., Luciano A.M., Franciosi F., Lodde V., Corbani D., and Modina S.C. (2011). The endothelial nitric oxide synthase/nitric oxide system is involved in the defective quality of bovine oocytes from low mid-antral follicle count ovaries. J Anim Sci.; 89(8): 2389 - 96.
[23] Yoshimura Y., Dharmarajan A.M., Gips S., Adachi T., Hosoi Y., Atlas S.J., and Wallach E.E. (1988). Effects of prostacyclin on ovulation and microvasculature of the in vitro perfused rabbit ovary. Am. J. Obst. Gyn., 159 (4): 977–82.