Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30169
Process Parameter Optimization for the Production of Gentamicin using Micromonouspora Echiniospora

Authors: M.Rajasimman, S.Subathra

Abstract:

The process parameters, temperature, pH and substrate concentration, were optimized for the production of gentamicin using Micromonouspora echinospora. Experiments were carried out according to central composite design in response surface method. The optimum conditions for the maximum production of gentamicin were found to be: temperature – 31.7oC, pH – 6.8 and substrate concentration – 3%. At these optimized conditions the production of gentamicin was found to be – 1040 mg/L. The R2 value of 0.9465 indicates a good fitness of the model.

Keywords: Gentamicin, Micromonouspora echinospora, response surface method, optimization, central composite design.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1072231

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1918

References:


[1] M. J. Weinstein, G. M. Luedemann, E. M. Oden, G. H. Wagman, J. P. Rosselet, J. A. Marquez, C. T. Coniglio, W. Charney, H. L. Herzog, and J. Black, "Gentamicin, new antibiotic complex from Micromonospora", J. Med. Chem., vol. 6, pp. 463-464, 1963.
[2] J. Chu, S. Zhang, Y. Zhuang, J. Chen, and Y. Li, "Factors affecting the biosynthesis and secretion of gentamicin", Process Biochem., vol.38, pp. 815-820, 2002.
[3] J. Chu, W. Niu, S. Zhang, Y. Zhuang, H. Hu, and Y. Li, "Effect of metal ions on the binding of gentamicin to the peptidoglycan of Micromonospora echinospora", Process Biochem., vol.39, pp.1145- 1150, 2004.
[4] M. Himabindu, R. Potumarthi, and A. Kitty, "Enhancement of gentamicin production by mutugenesis and non nutritional stress conditions in Micromonospora echinospora", Process Biochem., vol.42, pp. 1352-1356, 2007a.
[5] M. Himabindu, P. Ravichandra, and J. Annapurna, "Evaluation of immobilization conditions for enhanced production of gentamicin in repeated batch operation by micromonospora echinospora", Int. J. of chemical Reactor Engineering, vol.5, pp. 1-13, 2007 b.
[6] M. Himabindu, P. Ravichandra, and J. Annapurna, "Gentamicin production by micromonospora echinospora (Me-22) in stirred tank reactor: Effect of various parameters", Journal of basic microbiology, vol.48, pp. 53-58, 2007c.
[7] M. Rajasimman, and S. Subathra, "Optimization of gentamicin production: Comparison of RSM and ANN techniques", International journal of Natural and Engineering Science, vol. 2, no. 1, pp. 32-37, 2009.
[8] Y. C. Chang, C. L. Lee, and T. M. Pan, "Statistical optimization of media components for the production of Antrodia cinnamomea AC0623 in submerged cultures", Applied Microbiology and Biotechnology, vol.72, pp. 654-661, 2006.
[9] E. Kristo, C.G. Biliaderis, and N. Tzanetakis, "Modeling of the acidification process and rheological properties of milk fermented with a yogurt starter culture using response surface methodology", Food Chemistry, vol. 83, pp. 437 - 446, 2003.
[10] Q. K. Beg, R. K. Saxena, and R. Gupta, "Kinetic constants determination for an alkaline protease from Bacillus mojavensis using response surface methodology", Biotechnol. Bioeng., vol.78, pp. 289-295, 2002.
[11] L. S. T. Lai, C. C. Pan, and B. K. Tzeng, "The influence of medium design on lovastatin production and pellet formation with a highproducing mutant of Aspergillus terreus in submerged cultures", Process Biochem., vol.38, pp. 1317 - 1326, 2003.
[12] E. L. Soo, A. B. Salieh and M. Basri, "Response surface methodological study on lipase-catalyzed synthesis of amino acid surfactants", Process Biochem., vol.39, pp. 1511 - 1518, 2004.
[13] Y. X. Wang, and Z. X. Lu, "Optimization of processing parameters for the mycelial growth and extracellular polysaccharide production by Boletus spp. ACCC 50328", Process Biochem., vol.40, pp. 1043 - 1051, 2005.
[14] H. Wang, J. Ren, and Y. Zhang, "Use of p-dimethylaminobenzalhyde as a coloured reagent for determination of gentamycin", Talanta, Vol. 40, pp.851-853, 1993.