Study of Optical Properties of a Glutathione Capped Gold Nanoparticles Using Linker (MHDA) by Fourier Transform Infra Red Spectroscopy and Surface Enhanced Raman Scattering

Authors: A. Deręgowska, J. Depciuch, R. Wojnarowska, J. Polit, D. Broda, H. Nechai, M. Gonchar, and E. Sheregii

Abstract:

16-Mercaptohexadecanoic acid (MHDA) and tripeptide glutathione conjugated with gold nanoparticles (Au-NPs) are characterized by Fourier Transform InfaRared (FTIR) spectroscopy combined with Surface-enhanced Raman scattering (SERS) spectroscopy. Surface Plasmon Resonance (SPR) technique based on FTIR spectroscopy has become an important tool in biophysics, which is perspective for the study of organic compounds. FTIR-spectra of MHDA shows the line at 2500 cm-1 attributed to thiol group which is modified by presence of Au-NPs, suggesting the formation of bond between thiol group and gold. We also can observe the peaks originate from characteristic chemical group. A Raman spectrum of the same sample is also promising. Our preliminary experiments confirm that SERS-effect takes place for MHDA connected with Au-NPs and enable us to detected small number (less than 106 cm-2) of MHDA molecules. Combination of spectroscopy methods: FTIR and SERS – enable to study optical properties of Au- NPs and immobilized bio-molecules in context of a bio-nano-sensors.

Keywords: Glutathione; gold nanoparticles, Fourier transform infrared spectroscopy, MHDA, surface-enhanced Raman scattering.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1072088

References:

[1] S.J. Guo, E.K. Wang, "Synthesis and electrochemical applications of gold nanoparticles," Analytica Chimica Acta, Vol. 598, 2007, pp. 181- 192.
[2] Liu, D.Leech, H. Ju, "Application of Colloidal Gold in Protein Immobilization, Electron Transfer, and Biosensing," Analytical Letters, Vol. 36, , 2003, pp. 1-19.
[3] I. H. El-Sayed, X.Huang, M.A. El-Sayed, "Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: applications in oral cancer," Nano Letters. Vol. 5, 2005, pp. 829-834.
[4] M. Hu, J. Chen, ZY. Li, L. Au, G.V. Hartland, X. Li, M. Marquez, Y. Xia, "Gold nanostructures: engineering their plasmonic properties for biomedical applications, "Chemical Society Reviews, Vol. 35, 2006, pp.1084-1094.
[5] R.J. Silbey, R.A. Alberty, and M. G. Bawendi, Physical Chemistry, John Wiley & Sons Ltd., New York, 2004.
[6] R. Aroca, Surface Enhanced Vibrational Spectroscopy, John Wiley & Sons Ltd., Chichester, 2006.
[7] A.B. Descalzo, R. Martínez-Máñez, F. Sancenón, K. Hoffmann, K. Rurack, "Hybrid Nanoapatite by Polysaccharide Nanogel-templated Mineralization," Angewandte Chemie International Edition, Vol.45, 2006, pp. 5924-5948 2006.
[8] J. Kimling, M. Maier, B. Okenve, V. Kotaidis, H. Ballot, A. Plech, "Turkevich Method for Gold Nanoparticle Synthesis Revisited," Journal Physical Chemistry B, Vol. 110, 2006, pp. 15700-15707.
[9] N. Stasyuk, R. Serkiz, S. Mudry, G. Gayda, A. Zakalskiy, Y. Koval-chuk, M. Gonchar, M. Nisnevitch, "Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties," Nanotechnology Development, Vol.1, 2011, p. 11-15. S.
[10] V. Leff, L. Brandt, J. R. Heath, "Synthesis and Characterization of Hydrophobic, Organically-Soluble Gold Nanocrystals Functionalized with Primary Amines," Langmuir, Vol. 12, 1996, pp. 4723-4730.
[11] H.Shayani-Jam,D.Nematollahi,"Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N-acetylcysteine," Chemical Communications, Vol. 46, 2009, pp. 409-411.
[12] M. Hasan, D. Bethell, M. Brust, "The fate of sulfur-bound hydrogen on formation of self-assembled thiol monolayers on gold: (1)H NMR spectroscopic evidence from solutions of gold clusters," Journal of the American Chemical Society, Vol. 124, 2002, pp. 1132-1133.
[13] M. J. Hostetler, J.J. Stokes, R.W. Murray, "Infrared Spectroscopy of Three-Dimensional Self-Assembled Monolayers: N-Alkanethiolate Monolayers on Gold Cluster Compounds," Langmuir, Vol. 12, 1996, pp. 3604-3612.
[14] Y. Sahoo, H. Pizem, T. Fried, D. Golodnitsky, L. Burstein, C.N. Sukenik, G. Markovich, "Alkyl Phosphonate/Phosphate Coating on Magnetite Nanoparticles: A Comparison with Fatty Acids," Langmuir, Vol. 17, 2001, pp.7907-7911.