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Microbial Oil Production by Isolated Oleaginous Yeast Torulaspora globosa YU5/2
Abstract:Microbial oil was produced by soil isolated oleaginous yeast YU5/2 in flask-batch fermentation. The yeast was identified by molecular genetics technique based on sequence analysis of the variable D1/D2 domain of the large subunit (26S) ribosomal DNA and it was identified as Torulaspora globosa. T. globosa YU5/2 supported maximum values of 0.520 g/L/d, 0.472 g lipid/g cells, 4.16 g/L, and 0.156 g/L/d for volumetric lipid production rate, and specific yield of lipid, lipid concentration, and specific rate of lipid production respectively, when culture was performed in nitrogen-limiting medium supplemented with 80g/L glucose. Among the carbon sources tested, maximum cell yield coefficient (YX/S, g/L), maximum specific yield of lipid (YP/X, g lipid/g cells) and volumetric lipid production rate (QP, g/L/d) were found of 0.728, 0.237, and 0.619, respectively, using sweet potato tubers hydrolysates as carbon source.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1071822Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1828
 Fukuda, H., Kondo, A. and Noda, H. (2001) Biodiesel fuel production by transesterification of oils. Journal of Bioscience Bioengineering 92:405-416.
 Xue, F., Zhang, X., Luo, H. and Tan, T. (2006) A new method for preparing raw material for biodiesel production. Process Biochem 41:1699-1702.
 Li, Q., Du, W. and Liu, D. (2008) Perspectives of microbial oils for biodiesel production. Appl Microbiol Biotechnol 80:749-756.
 Ratledge, C. and Wynn, J.P. (2002) The biochemistryand molecular biology of lipid accumulation in oleaginous microorganisms. Adv Appl Microbiol 51:1-51.
 Tehlivets, O., Scheuringer, K. and Kohlwein, S.D. (2007) Fatty acid synthesis and elongation in yeast. Biochimica et Biophysica Acta 1771:255-270.
 Evan, C.T. and Ratledge, C. (1984) Influence of nitrogen metabolism on lipid accumulation in oleaginous yeasts. J Gen Microbiol 130:1693-1704.
 Meng, X., Yang, J., Xu, X., Zhang, L., Nie, Q. and Xian, M. (2009) Biodiesel production from oleaginous microorganisms. Renew Energy 34:1- 5.
 Lee, J.M. (1992) Biochemical Engineering. Prentice Hall international, New Jersey.
 Kurtzman, C.P. and Fell, J.W. (1998) The Yeasts: A Taxonomic Study. 4thRevised and Enlarged Edition. Elsevier Science Publisher, Amsterdam.
 Kwon, D.Y. and Rhee, J.S. (1986) A Simple and rapid colorimetric method for determination of free fatty acids for lipase assay. J Am Oil Chem Soc 63:89-92.
 Zhu, L.Y., Zong, M.H. and Wu, H. (2008) Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation. Bioresour Technol 99:7881-7885.
 Li, Y., Zhao, Z. and Bai, F. (2007) High cell-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture. Enzyme Microbiology Technology 41:312-317.
 Zhao, C.H., Zhanga, T., Li, M. and Chia, Z.M. (2010) Single cell oil production from hydrolysates of inulin and extract of tubers of Jerusalem artichoke by Rhodotorula mucilaginosa TJY15a. Process Biochemistry 45:1121-1126.