Commenced in January 2007
Paper Count: 31836
Protein Delivery from Polymeric Nanoparticles
Abstract:Aim of this work was to compare the efficacy of two loading methods of proteins onto polymeric nanocarriers: adsorption and encapsulation methods. Preliminary studies of protein loading were done using Bovine Serum Albumin (BSA) as model protein. Nanocarriers were prepared starting from polylactic co-glycolic acid (PLGA) polymer; production methods used are two different variants of emulsion evaporation method. Nanoparticles obtained were analyzed in terms of dimensions by Dynamic Light Scattering and Loading Efficiency of BSA by Bradford Assay. Loaded nanoparticles were then submitted to in-vitro protein dissolution test in order to study the effect of the delivery system on the release rate of the protein.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1071816Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2231
 M. Singh, R. Sharma and U.C. Banerjee, "Biotechnological applications of cyclodextrins" Biotechnol Adv, vol. 20; no 5-6, pp.341-359, 2002.
 P. Couvreur and C. Vauthier, " Nanotechnology: Intelligent design to treat complex disease", Pharm Res, vol 7, no, 23, pp 1417-1450, July 2006
 M.F. Francis, M. Cristea, F.M. Winnik, "Polymeric micelles for oral drug delivery: Why and how", Pure Appl Chem, vol.76 , no.7-8, pp1321- 1335, 2004;.
 R.C. Mundargi, V.R. Babu, V. Rangaswamy, P. Patel and T.M. Aminabhavi "Nano/micro technologies for delivering macromolecular therapeutics using poly(D,L-lactide-co-glycolide) and its derivatives" J Control Rel, Vol.125,no.3, pp 193-209, 2008.
 C. Pinto-Reis, R.J. Neufeld, A.J. Ribeiro and F. Veiga "Nanoencapsulation II. Biomedical applications and current status of peptide and protein nanoparticulate delivery systems", Nanomedicine, vol.2,no.2 pp 53- 65; June 2006.
 K. Tahara, T. Sakai, H. Yamamoto, H. Takeuchi and Y. Kawashima, "Establishing chitosan coated PLGA nanosphere platform loaded with wide variety of nucleic acid by complexation with cationic compound for gene delivery", Int J Pharm, no. 354, pp 210-216, February 2008;.
 D. Lamalle-Bernard, S. Munier, C. Compagnon, M.H. Charles, V.S. Kalyanaraman, T. Delair, B. Verrier and Y. Ataman-┼Énal, "Coadsorption of HIV-1 p24 and gp120 proteins to surfactant-free anionic PLA nanoparticles preserves antigenicity and immunogenicity", J Control Rel, no. 115, pp. 57- 67, July 2006.
 C. Cai, U. Bakowsky, E. Rytting, A.K. Schaper and T. Kissel, "Charged nanoparticles as protein delivery systems: A feasibility study using lysozyme as model protein", Eur J Pharm Biopharm, no. 69, pp. 31-42, 2008.
 M.A. Dobrovolskaia and S.E. McNeil, "Immunological properties of engineered nanomaterials", Nat Nanotechnol, vol 2, pp. 469-478, August 2007;
 P Gong and I Szleifer, "Competitive adsorption of model charged proteins: the effect of total charge and charge distribution", J Colloid Interface Sci, no. 278, pp. 81-90, June 2004.
 J.H. Kim, A. Taluja, K. Knutson, Y.H. Bae, "Stability of bovine serum albumin complexed with PEG-poly(L-histidine) diblock copolymer in PLGA microspheres", J Control Rel, no. 109, pp. 86-100, November 2005.
 I. Lynch, T. Cedervall, M. Lundqvist, C. Cabaleiro-Lago, S. Linse and K.A. Dawson, "The nanoparticle-protein complex as a biological entity; a complex fluids and surface science challenge for the 21st century", Adv Colloid and Interface Sci, Vol 134-135, pp. 167-174, October 2007.
 E.D. Kaufman, J. Belyea, M.C. Johnson, Z.M. Nicholson, J.L. Ricks, P.K. Shah, M. Bayless, T. Pettersson, Z. Feldoto, E. Blomberg, P. Claesson and S. Franzen, "Probing protein adsorption onto mercaptoundecanoic acid stabilized gold nanoparticles and surfaces by quartz crystal microbalance and ╬Â-potential measurements", Langmuir, no. 23, pp.6053-6062, March 2007.