Commenced in January 2007
Paper Count: 31103
Biodiesel Fuel Production by Methanolysis of Fish Oil Derived from the Discarded Parts of Fish Catalyzed by Carica papaya Lipase
Abstract:In this paper, naturally immobilized lipase, Carica papaya lipase, catalyzed biodiesel production from fish oil was studied. The refined fish oil, extracted from the discarded parts of fish, was used as a starting material for biodiesel production. The effects of molar ratio of oil: methanol, lipase dosage, initial water activity of lipase, temperature and solvent were investigated. It was found that Carica papaya lipase was suitable for methanolysis of fish oil to produce methyl ester. The maximum yield of methyl ester could reach up to 83% with the optimal reaction conditions: oil: methanol molar ratio of 1: 4, 20% (based on oil) of lipase, initial water activity of lipase at 0.23 and 20% (based on oil) of tert-butanol at 40oC after 18 h of reaction time. There was negligible loss in lipase activity even after repeated use for 30 cycles.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1071468Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3023
 K. Annapurna, M. Paramita, G. K. Vijay and B. Rintu, "Enzymatic transesterification of Jatropha oil," Biotech& Biofuels, vol. 2, no page, 2009.
 N. Kaili, X. Feng, W. Fang and T. Tianwei, "Lipase catalyzed methanolysis to produce biodiesel: optimization of biodiesel production," J. Mol. Catal. B: Enzym. Vol 43, 142-147, 2006.
 W. Hong, Z. Min-hua, L. Qian and W. Hua-chang, "Enzymatic conversion of waste oil to biodiesel in a solvent-free system," Prepr. Pap. Am. Chem. Soc., Div. Fuel Chem. Vol. 48, 533-534, 2003.
 R. Alcantara, J. Amores, L. Canoira, E. Fidalgo, M. J. Franco and A. Navarro, "Catalytic production of biodiesel from soy-bean oil, used frying oil and tallow," Biomass & Bioenergy. Vol 18, 515-527, 2000.
 A. Robles-Medina, P. A. Gonzalez-Moreno, L. Esteban-R Cerdan and E. Molina-Grima, "Biocatalysis: Towards ever greener biodiesel production," Biotechnology Advances., vol. 27, pp. 398-408, 2009.
 L. Lilin, D. Wei, L. Dehua, W. Li and L. Zebo, "Lipase-catalyzed transesterification of rapeseed oils for biodiesel production with a novel organic solvent as the reaction medium," J. Mol. Catal. B: Enzym., vol. 43, 58-62, 2006.
 G. Steinke, R. Kirchhoff and K.D. Mukherjee, "Lipase-catalyzed alcoholysis of crambe oil and camelina oil for the preparation of longchain esters," J. Am. Oil Chem. Soc., vol. 69, 166-169, 2000.
 Y. Watanabe, Y. Shimada, A. Sugihara, H. Noda, H. Fukuda and Y. Tominaga, "Continuous production of biodiesel fuel from vegetable oil using immobilized Candida antarctica lipase," J. Am. Oil Chem. Soc., vol. 77, 355-360, 2000.
 W. Du, W. Li, T. Sun, X. Chen and D. Liu, "Perspectives for biotechnological production of biodiesel and impacts," Appl Microbiol Biotechnol, vol. 79, 331-337, 2008.
 Y. Shimada, Y. Watanabe, A. Sugihara and Y. Tominaga, "Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing," J. Mol. Catal. B: Enzym., vol. 17, 133-142, 2002.
 O. Kose, M. Tuter and H. A. Aksoy, "Immobilized Candida Antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium," Bioresour. Technol., vol. 83, 125-129, 2002.
 P. Villeneuve, A. Skarbek, M. Pina, J. Graille and T. A. Foglia, "Catalytic behavior of Carica papaya latex in transesterification reactions," Biotechnol. Tech., vol. 11, 637-639, 1997.
 M. Kaieda, T. Samukawa, T. Matsumoto, K. Ban, A. Kondo, Y. Shimada, H. Noda, F. Nomoto, K. Ohtsuka, E. Izumoto and H. Fukuda, "Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water containing system without an organic solvent," J. Biosci. Bioeng., vol. 88, 627-631, 1999.
 K. Ban, M. Kaieda, T. Matsumoto, A. Kondo and H. Fukuda, "Whole cell biocatalysts for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles," Biochem. Eng. J., vol. 8, 39-43, 2001.
 T. A. Foglia and P. Villeneuve, "Carica papaya latex-catalyzed synthesis of structured triacylglycerol," J. Am. Oil Chem. Soc., vol. 74, 1447- 1450, 1997.
 P. Angkanurukpun, P. Sriburi and P. Kanasawud, "Improvement of Carica papaya lipase for methanolysis of triolein," Chiang Mai J. Sci., vol. 33, 217-222, 2006.
 L. Cherng-Yuan and L. Rong-Ji, "Fuel properties of biodiesel produced from the crude fish oil from the soapstock of marine fish," Fuel Processing Technology., 130-136, 2008.
 D. Y. Kwon and J. S. Rhee, "A simple and rapid colorimetric method for determination of free fatty acids for lipase assay," J. Am. Oil Chem. Soc., vol. 63, 89-92, 1986.
 A. S. Ramadhas, S. Jayaraj and C. Muraleedharan, "Biodiesel production from high FFA rubber seed oil," Fuel., Vol. 84, 335-340, 2005.
 M. Canakci and J. G. Van, "Biodiesel production from oils and fats with high free fatty acids," Transactions of the ASAE., vol. 44, 1429-1436, 2001b.
 M. Canakci and J. G. Van, "A pilot plant to produce biodiesel from high free fatty acid feedstocks," ASAE, 2001a.
 K. Krisnangkura, T. Yimsuwan and R. Pairintra, "An empirical approach in predicting biodiesel viscosity at various temperature," Fuel., vol. 85, 107-113, 2006.
 L. A. Nelson, T. A. Foglia and W. N. Marmer, "Lipase catalyzed production of biodiesel," J. Am Oil Chem Soc., vol. 73, 1191-1195, 1996.
 V. Dossat, D. Combes and A. Marty, "Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor: influence of the glycerol production," Enzyme Microb Technol., vol. 25, 194-200, 1999.
 L. Jike, N. Kaili, X. Feng, W. Fang and T. Tianwei, "Enzymatic synthesis of fatty acid methyl esters from lard with immobilized Candida sp. 99-125," Process Biochemistry., vol. 42, 1367-1370, 2007.