Commenced in January 2007
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Effects of pH, Temperature, Enzyme and Substrate Concentration on Xylooligosaccharides Production
Abstract:Agricultural residue such as oil palm fronds (OPF) is cheap, widespread and available throughout the year. Hemicelluloses extracted from OPF can be hydrolyzed to their monomers and used in production of xylooligosaccharides (XOs). The objective of the present study was to optimize the enzymatic hydrolysis process of OPF hemicellulose by varying pH, temperature, enzyme and substrate concentration for production of XOs. Hemicelluloses was extracted from OPF by using 3 M potassium hydroxide (KOH) at temperature of 40°C for 4 hrs and stirred at 400 rpm. The hemicellulose was then hydrolyzed using Trichoderma longibrachiatum xylanase at different pH, temperature, enzyme and substrate concentration. XOs were characterized based on reducing sugar determination. The optimum conditions to produced XOs from OPF hemicellulose was obtained at pH 4.6, temperature of 40°C , enzyme concentration of 2 U/mL and 2% substrate concentration. The results established the suitability of oil palm fronds as raw material for production of XOs.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1071406Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2690
 PEMANDU (Performance Management and Delivery Unit of Malaysia), "NKEA: Palm oil and rubber," ETP Annual Report, 2011.
 MPOB (Malaysian Palm Oil Board), "Overview of the oil palm industry 2011," February 2012.
 Malaysian Innovation Agency, "National Biomass strategy 2020: New wealth creation for Malaysia-s palm oil industry," 2011.
 S. H. Shuit, K. T. Tan, K. T. Lee, A. H. Kamarudin, "Oil palm biomass as a sustainable energy sorce: A Malaysian case study," Energy, vol. 34, pp. 1225-1235, 2009.
 F. Y. Ng, F. K. Yew, Y. Basiron, K. Sundram, "A renewable future driven with Malaysian palm oil-based green technology," Journal of Palm Oil and The Environment, vol. 2, pp. 1-7, 2011.
 K. O. Lim, Z. A. Zainal, G. A. Quadir, M. Z Abdullah, "Plant based energy potential and biomass utilization in Malaysia," International Energy Journal, vol. 1, pp. 77-88, 2000.
 N. Mohd Nor, "The effect of hydrothermal treatment on the physic-chemical properties of oil palm fronds (OPF) derived hemicelluloses," Master thesis, School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia, 2008.
 R. L. Howard, E. Abotsi, E. L. Jansen van Rensburg, S. Howard, "Lignocellulosic biotechnology: issues of bioconversion and enzyme production," African Journal of Biotechnology, vol. 2 (12), pp. 602-619, December 2003.
 O. Akpinar, K. Erdogan, and S. Bostanci, "Enzymatic production of xylooligosaccharide from selected agricultural wastes," Food and Bioproducts Processing, vol. 87, pp. 145-151, 2009.
 S. Caparr├│s, G. Garrote, J. Ariza, M. J. D├¡az, F. L├│pez, "Xylooligosaccharides production from Arundo donax," Journal of Agricultural and Food Chemistry, vol. 55, pp. 5536-5543, 2007.
 M. J. V├ízquez, J. L. Alonso, H. Dom├¡nguez, P. C. Paraj├│, "Xylooligosaccharides: manufacture and application," Trends in Food Science and Technology, vol. 11, pp. 387-393, 2000.
 S. Mumtaz, "Production of xylooligosaccharides from agricultural waste for preparation of synbiotic food," Master thesis, National Institute of Food Science and Technology, University of Agriculture Faisalabad, Pakistan, 2009.
 S. Sabiha Hanim, M. A. Mohd Nor, A. Rosma, "Effect of autohydrolysis and enzymatic treatment on oil palm (Elais Guineensis Jacq.) frond fibres for xylose and xylooligosaccharides production," Bioresource Technology, vol. 102, pp. 1234-1239, 2011.
 O. Akpinar, O. Ak, A. Kavas, U. Bakir, L. Yilmaz, "Enzymatic production of xylooligosaccharides from cotton stalks,"Journal of Agricultural and Food Chemistry, vol. 55, pp. 5544-5551, 2007.
 M. Anis, "Extraction and functional properties of oil palm biomass," Ph.D thesis, University Sains Malaysia, Penang, Malaysia, 2000.
 M. J. Bailey, P. Biely, and K. Poutanen, "Interlaboratory testing of methods for assay of xylanase activity," Journal of Biotechnology, vol. 23, pp. 257-270, 1992
 R. L. Burner, "Determination sugar value in method in carbohydrate chemistry IV (Roy I Whistle, Robert, J Smith & James, N. Bemiller, eds)," New York: Academic Press, pp. 65-71, 1964.
 S. Subramaniyam and P. Prema, "Boitechnology of microbial xylanases:Enzymology, Molecular Biology, and Application," Critical Reviews in Biotechnology, vol. 22 (1), pp. 36-46, 2002.
 O. Akpinar and S. Bostanci, "Xylooligosaccharide production from lignocellulosic wastes with Trichoderma longibrachiatum xylanase," Journal of Food, Agriculture and Environment, vol.7 (1), pp. 70-74, 2009.
 N. Jayapal, A. K. Samanta, A. P. Kolte, S. Senai, M. Sridhar, K. P. Suresh, K. T Sampath, "Value addition to sugarcane bagasse: Xylan extraction and its process optimization for xylooligosaccharides production,"Industrial Crops and Products, 42, pp, 14-24, 2013.
 J. Jaskari, P. Kontula, A.Siitonen, H. Jousimies-Somer, T. Mattila-Sandholm, K. Poutanen, "Oat glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains," Appl. Microbial. Biotechnol., vol. 49, pp. 175-181, 1998.
 C. Teng, Q. Yan, Z. Jiang, G. Fan, B. Shi, "Production of xylooligosaccharides from the steam explosion liquor of corncobs coupled with enzymatic hydrolysis using a thermostable xylanase," Bioresour. Technol., vol. 101, pp. 7679-7682, 2010.
 Q.P. Yuan, H. Zhang, Z.M. Qian, X.J. Yang, "Pilot-plant production of xylooligosaccharides from corncob by steaming, enzymatic hydrolysis and nanofiltration," J. Chem. Technol. Biotechnol., vol. 79, pp. 1073-1079, 2004.
 S.Q. Yang, Q. J. Yan, Z. Q. Jiang, L.T. Li, H. M. tian, Y.Z. Wang, "High-level of xylanase production by the thermophilic paecilomyces thermophilia J18 on wheat straw in solid-state fermentation," Bioresour. Technol., vol. 97, pp. 1794-1800, 2006.
 K.Y Yoon, E.E. Woodams,Y. D Hang, "Enzymatic production of pentose from the hemicellulose fraction of corn residues," Food Science and Technology, vol. 39, pp. 388-392, 2006.
 Y. Sun and J. Cheng, "Hydrolysis of lignicellulosic materials for ethanol production: A review," Bioresource Technology, vol. 83, pp. 1-11, 2002.