An Immunosensor for Bladder Cancer Screening

Authors: Congo Tak Shing Ching, Hong-Sheng Chen, Tai-Ping Sun, Hsiu-Li Shieh

Abstract:

Nuclear matrix protein 22 (NMP22) is a FDA approved biomarker for bladder cancer. The objective of this study is to develop a simple NMP22 immumosensor (NMP22-IMS) for accurate measurement of NMP22. The NMP22-IMS was constructed with NMP22 antibody immobilized on screen-printed carbon electrodes. The construction procedures and antibody immobilization are simple. Results showed that the NMP22-IMS has an excellent (r2³0.95) response range (20 – 100 ng/mL). In conclusion, a simple and reliable NMP22-IMS was developed, capable of precisely determining urine NMP22 level.

Keywords: Bladder Cancer, Immunosensor, Impedance, NMP22

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1071286

References:

[1] Department of Health, Executive Yuan, R.O.C. (TAIWAN), "Health Statistics in Taiwan, 2009," 2009. Last accessed: 23rd March 2012. Available at: http://www.doh.gov.tw/CHT2006/DisplayStatisticFile.aspx?d=76512&s =1
[2] GLOBOCAN 2008´╝êIARC´╝ë Section of Cancer Information, "WORLD - Most frequent cancers: both sexes," 2008. Last accessed: 23rd March 2012. Available at: http://globocan.iarc.fr/factsheets/populations/factsheet.asp?uno=900
[3] N. Gan, L.Y. Wang, W.M. Xu, T.H. Li, Q.L. Jiang, "Electrochemical Immuno-Biosensor for the Rapid Determination of Nuclear Matrix Protein 22 (NMP22) antigen in Urine Samples by Co(III) Phthlocyanine/Fe3O4/Au Collide Coimmobilized Electrode," Chin J Anal Chem, vol. 35, pp. 1553-1558, November 2007.