The Comparation of Activation Nuclear Factor Kappa Beta (NFKB) at Rattus Novergicus Strain Wistar Induced by Various Duration High Fat Diet (HFD)

Authors: Titin Andri Wihastuti, Djanggan Sargowo

Abstract:

NFκB is a transcription factor regulating many function of the vessel wall. In the normal condition , NFκB is revealed diffuse cytoplasmic expressionsuggesting that the system is inactive. The presence of activation NFκB provide a potential pathway for the rapid transcriptional of a variety of genes encoding cytokines, growth factors, adhesion molecules and procoagulatory factors. It is likely to play an important role in chronic inflamatory disease involved atherosclerosis. There are many stimuli with the potential to active NFκB, including hyperlipidemia. We used 24 mice which was divided in 6 groups. The HFD given by et libitum procedure during 2, 4, and 6 months. The parameters in this study were the amount of NFKB activation ,H2O2 as ROS and VCAM-1 as a product of NFKB activation. H2O2 colorimetryc assay performed directly using Anti Rat H2O2 ELISA Kit. The NFKB and VCAM-1 detection obtained from aorta mice, measured by ELISA kit and imunohistochemistry. There was a significant difference activation of H2O2, NFKB and VCAM-1 level at induce HFD after 2, 4 and 6 months. It suggest that HFD induce ROS formation and increase the activation of NFKB as one of atherosclerosis marker that caused by hyperlipidemia as classical atheroschlerosis risk factor.

Keywords: High Fat Diet, NFKB, H2O2, atherosclerosis

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1070947

References:

[1] G. O. Young, "Synthetic structure of industrial plastics (Book style with paper title and editor)," in Plastics, 2nd ed. vol. 3, J. Peters, Ed. New York: McGraw-Hill, 1964, pp. 15-64.
[2] S.A. Price, " Patofisiologi : Konsep Klinis Proses Penyakit", Edisi 6. Jakarta : EGC, 2006.
[3] L.A. Reynolds, "Cholesterol, Atherosclerosis And Coronary Disease In The UK 1950-2000,"Volume 27. London : Wellcome Trust Centre, 2006.
[4] H. Sulastomo, "Sindroma Koroner Akut dengan gangguan Metabolik pada Wanita Usia Muda Pengguna Kontrasepsi Hormonal", Departement of Cardiology and Vascular Faculty of Medicine University of Indonesia, 2010.
[5] D. Sargowo, "Atherosklerosis Its Manifestation and Danger", FK Universitas Brawijaya, Malang, 2002.
[6] P.A. Mayes, 2003 "Lipid Yang Memiliki Makna Fisiologis" Jakarta : EGC, 2003.
[7] G. Bonizzi , "The Two NF╬║B activation pathway and their role in innate and adaptive immunity", Trends Immunol, 2004, 25 : 280-288.
[8] C. Monaco, " T cell-mediated signalling in immune, inflammatory and angiogenic processes: the cascade of events leading to inflammatory diseases. Curr Drug Targets" Inflamm Allergy; 2003, 84:695.
[9] S.X. Chen, "Active constituents against HIV-1 protease from Garcinia mangostana" Planta Med, 2005, 62(4):381-2.
[10] E. Andreakos. 2003" Heterogeneous requirement of IkB kinase 2 for inflammatory cytokine and matrix metalloproteinase production in rheumatoid arthritis: implications for therapy."Arthritis Rheum, 2005,48:1901-12.
[11] Goldstein, "Molecular Medicine, The Cholesterol Quartet", 2001, Science.
[12] S.H. Wilson,"Activated nuclear factorkappaB is present in the coronary vasculature in experimental hypercholesterolemia. Atherosclerosis" 2000, 148:23- 30.
[13] Ridker,"Inflamation and atherosclerosis", Circulation , 2002, 115 : 1135-1143.