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Biofungicide Trichodex WP

Authors: Snezana Rajkovic, Miroslava Markovic, Radoslav Rajkovic, Ljubinko Rakonjac


Grey mold on grape is caused by the fungus Botrytis cinerea Pers. Trichodex WP, a new biofungicide, that contains fungal spores of Trichoderma harzianum Rifai, was used for biological control of Grey mold on grape. The efficacy of Trichodex WP has been reported from many experiments. Experiments were carried out in the locality – Banatski Karlovac, on grapevine species – talijanski rizling. The trials were set according to instructions of methods PP1/152(2) and PP1/17(3) , according to a fully randomized block design. Phytotoxicity was estimated by PP methods 1/135(2), the intensity of infection according to Towsend Heuberger , the efficiency by Abbott, the analysis of variance with Duncan test and PP/181(2). Application of Trichodex WP is limited to the first two treatments. Other treatments are performed with the fungicides based on a.i. procymidone, vinclozoline and iprodione.

Keywords: Biofungicides, efficacy, grey mold, Trichodex WP.

Digital Object Identifier (DOI):

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[1] Y. Elad, B. Williamson, P. Tudzynski, N Delen, "Botrytis spp. and diseases they cause in agricultural systems - an introduction". In: Elad Y, Williamson B, Tudzynski Pand Delen N (eds.) Botrytis: Biology, Pathology and Control, 2004., pp. 1-8. Kluwer Academic Publishers, Dordrecht, The Netherlands.
[2] W.G. Yi, S.E. Law and H.Y. Wetzstein "Fungicide sprays can injure the stigmatic surface during receptivity in almond flowers". Annals of Botany vol. 91, pp. 335-341. 2003.
[3] F. Dianez, M. Santos , R Blanco, JC. Tello , "Fungicide resistance in Botrytis cinerea isolates from strawberry crops in Huelva (southwestern Spain)". Phytoparasitica, vol.30, pp. 529-534. 2002.
[4] T. Hunter, K.J. Brent, G.A. Carter and J.A. Hutcheon "Effects of fungicide spray regimes on incidence of dicarboximide resistance in grey mold (Botrytis cinerea) on strawberry plants". Annals of Applied Biology vol. 110, pp. 515, 1987.
[5] Y. Elad, H. Yunis, T. Katan, "Multiple fungicide resistance to benzimidazoles, dicarboximides and diethofencarb in field isolates of Botrytis cinerea in Israel". Plant Pathology, vol. 41, pp. 41-44. 1992.
[6] G.W. Moorman & R.J. Lease "Benzimidazole-and dicarboximide-resistant Botrytis cinerea" from Pennsylvania greenhouses. Plant Disease, vol.76. pp. 477-480. 1992.
[7] S. Rajkovic "Pojava rezistentnosti Botrytis cinerea Pers. u vinovoj lozi na fungicide iz grupe benzimidazola, dikarboksimida i dihlofluanida". VIII Yugoslav Symposium on Plant Protection, 0patija, Plant Protection Bulletin vol. 334, pp. 9-10. 1990.
[8] S. Rajkovic, D. Matijevic, M. Rosic, "Eksperimentalna ispitivanja u suzbijanju sive trulezi grozdja (Botrytis cinerea Pers.) " in the 1991in Banatski Karlovac. XVI Seminar of Plant Protection of Serbia, Zlatibor, Proceedings, pp. 90-91. 1992a.
[9] S. Rajkovic "Rezistentnost Botrytis cinerea Pers. prema nekim fungicidima koriscenim u vinovoj lozi". 9th Yugoslav Symposium on Plant Protection, Vrnjacka Banja, 1992b. Proceedings, pp. 111-112..
[10] S. Rajkovic "Rezistentnost Botrytis cinerea Pers. na neke fungicide koji se koriste u zastiti vinove loze". Master's thesis, University of Belgrade. Faculty of Agriculture, Belgrade-Zemun, 1992c, pp.1-75.
[11] Y. Elad, K. Evensen, "Physiological aspects of resistance to Botrytis cinerea". Phytopathology, vol. 85. pp. 637-643.1995.
[12] S. Gielen, R. Aerts & B. Seel "Different products for biological control of Botrytis cinerea examined on wounded stem tissue of tomato plants". Communication Agricultural Applied Biological Sciences, vol. 69. pp. 641-647. 2004.
[13] T.M. O'neill, A. Niv, Y. Elad & D. Shtienberg "Biological control of Botrytis cinerea on tomato stem wounds with Trichoderma harzianum". European Journal Plant Pathology, vol. 102, pp. 635-643. 1996.
[14] S. Haram, H. Schickler, A. Oppenheim & I. Chet "Differentia expression of Trichoderma harzianum chitinases during mycoparasitism". Phytopathology, vol. 86, pp. 980- 985, 1996.
[15] N.D. Paul, R.J. Jacobson, A. Taylor, J.J. Wargent & J.P. "Moore The use of wavelength-selective plastic cladding materials in horticulture: understanding of crop and fungal responses through the assessment of biological spectral weighting functions. Photochemistry and Photobiology, vol. 81, pp. 1052-1060. 2005.
[16] G. Zimand, Y. Elad & I. Chet "Effect of Trichoderma harzianum on Botrytis cinerea pathogenicity". Phytopathology, vol. 86, pp. 1255- 1260. 1996.
[17] Y. Elad, " Trichodema harzianum T39 preparation for biocontrol of plant diseases -control of Botrytis cinerea, Sclerotinia sclerotiorum and Cladosporium fulvum." - Biocontrol Sci. Technol. vol. 10, pp. 499-507. 2000.
[18] Y. Elad, S. Freeman, "Biological control of fungal plant pathogens". In: Kempken F (ed.)The Mycota, A comprehensive treatise on fungi as experimental systems for basic and applied research. XI. - Agricultural applications (pp 93-109) Springer, Heidelberg, Germany. 2002.
[19] S. Freeman, D. Minz, I. Kolesnik, O. Barbul, A. Zveibil, M. Maymon, Y. Nitzani, B. Kirshner, Rav-D. David, A. Bilu, A. Dag, S. Shafir,Y. Elad, "Trichoderma biocontrol of Colletotrichum acutatum and Botrytis cinerea and survival in strawberry". European Journal of Plant Pathology. 2004. vol.110, pp. 361-370.
[20] A. Trosmo and C. Dennis "The use of Trichoderma species to control strawberry fruit rots". Netherlands Journal of Plant Pathology vol. 83, pp. 449-455. 1977.
[21] EPPO, "Guidelines for the efficacy evaluation of plant protection products: Phytotoxicity assessment" - PP 1/135(2), in EPPO Standards: "Guidelines for the efficacy evaluation of plant protection products", vol. 1, EPPO, Paris, 1997, pp. 31-36.
[22] EPPO, "Guidelines for the efficacy evaluation of plant protection products: Design and analysis of efficacy evaluation trials" - PP 1/152(2), in EPPO Standards: "Guidelines for the efficacy evaluation of plant protection products", vol. 1, EPPO, Paris, 1997, pp. 37-51.
[23] EPPO, "Guidelines for the efficacy evaluation of plant protection products: Botrytis cinerea on grape" - PP 1/17(3), in EPPO Standards: Guidelines for the efficacy evaluation of plant protection products, vol. 1, EPPO, Paris. 1997, pp. 63-67.
[24] H. H. Mckinney "Influence of soil temperature and moisture on infection of wheat seedlings by Helminthosporium sativum". Journal Agricultural Research, vol. 26, pp. 195-217. 1923.
[G.R. Towsend and J.W. Heuberger "Methods for estimating losses cansed by diseases in fungicide experiments". Plant Dis. Rep. 24, pp. 340-343. 1943.
[26] W.S. Abbott, "A method for computing the effectiveness of an insecticide". J Econ Entomol. vol. 18, pp. 265-267. 1925.
[27] D.B. Duncan, "Multiple-range and multiple F test". Biometrics, vol. 11, pp. 1-42. 1955.
[28] EPPO, "Guidelines for the efficacy evaluation of plant protection products: Conduct and reporting of efficacy evaluation trials" - PP 1/181(2), in EPPO Standards: "Guidelines for the efficacy evaluation of plant protection products", vol. 1, EPPO, Paris, 1997, pp. 52-58.