Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30184
Determination of Alkaline Protease Production In Serratia Marcescens Sp7 Using Agro Wastes As Substrate Medium, Optimization Of Production Parameters And Purification Of The Enzyme

Authors: Baby Joseph, Sankarganesh Palaniyandi

Abstract:

The enzyme alkaline protease production was determined under solid state fermentation using the soil bacteria Serratia marcescens sp7. The maximum production was obtained from wheat bran medium than ground nut shell and chemically defined medium. The physiological fermentation factors such as pH of the medium (pH 8), Temperature (40oC) and incubation time (48 hrs) played a vital role in alkaline protease production in all the above. 100Mm NaCl has given better resolution during elution of the enzymes. The enzyme production was found to be associated with growth of the bacterial culture.

Keywords: Alkaline protease, Wheat bran, Ground nut shell, Serratia marcescens

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1070357

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2114

References:


[1] R. Gupta, Q. K. Beg, S. Khan, and B. Chauhan, "An overview on fermentation, downstream processing and properties of microbial alkaline proteases,", Appl. Microbiol. Biotech. 60, 381-395, 2002a
[2] R. Gupta, Q.K. Beg, P. Lorenz, (2002b), "Bacterial Alkaline Protease: Molecular approaches and industrial application," Appl. Microbial. Biotechnol., 59, 15-32, 2002b.
[3] Francisco, J. Ust├íriz, L. Adriana, A. G. Luis, A.G, Mario, D, "Fermentation conditions increasing protease production by Serratia marcescens in fresh whey," Rev. Téc. Ing. Univ. Zulia. 31(1), 79-89, 2008.
[4] M. Kalpana Devi, B.A. Rasheedha, G.R. Gnanaprabhal, B.V.Pradeep, M. Palaniswamy, "Purification, characterization of alkaline protease enzyme from native isolate Aspergillus niger and its compatibility with commercial detergents," Ind. J. Science. Tech. 1(7), 1-6, 2008
[5] Karl-Heinz Maurer, "Detergent proteases," Curr. Opi. Biotech. 15, 330-334, 2004.
[6] D.P.N. Ramakrishna, N. Gopi Reddy, S.V. Rajagopal S.V, "Purification and Properties of an Extra Cellular Alkaline Protease Produced By Bacillus Subtilis (MTTC N0-10110)". Internat. J. Biotech. Biochem. 6(4), 493-504, 2010.
[7] Hamid mukhtar, Ikram-ul-haq, "Production of alkaline protease by Bacillus subtilis and its application as a depilating agent in leather processing," Pak. J. Bot. 40(4), 1673-1679, 2008.
[8] Vishalakshi, N.; Lingappa, K.; Amena, S.; Prabhakar, M.; Dayanand. (2009). Production of alkaline protease from Streptomyces gulbargensis and its application in removal of blood strains. Ind. J. Biotech. 8, 280-285.
[9] A. Sumantha, C. Larroche, P. Ashok, "Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective," Food Technol. Biotechnol. 44 (2), 211-220, 2006.
[10] J. R. Simkhada, M. Poonam, S.C. Seung, C.Y. Jin, "A novel fibrinolytic protease from Streptomyces sp. CS684" Process Biochem. 45, 88- 93, 2010.
[11] P. Bjorn, Johansson, S. Oonagh, B. Lars, IdeS: A Bacterial Proteolytic Enzyme with Therapeutic Potential. PLoS ONE 3(2), e1692. doi:10.1371/ journal.pone.0001692, 2008.
[12] R. Selvakumar, Sathis kumar, K. Swaminathan, "Purification and wash performance analysis of thermostable extracellular alkaline protease produced by soil bacterium bacillus sp.GOS-2". Asia. J. Microbiol. Biotech. Env. Sci. 10, 29-35, 2008.
[13] O.H. Lowry, N. Rosebrough, A. L. Farr, R.L. Ronadall, " Protein measurement with the folin phenol reagent," J. Biol. chem. 193, 265-273, 1951.
[14] R.S.Prakasham, R.C. Subba, P.N. Sharma, Green gram huskÔÇöan inexpensive substrate for alkaline protease production by Bacillus sp. in solidstate fermentation," Bioresour. Tech. 97, 1449-1454, 2006.
[15] R. Paranthaman, K. Alagusundaram, J. Indhumathi, "Production of Protease from Rice Mill Wastes by Aspergillus niger in Solid State Fermentation," Worl. J. Agri. Sciences. 5 (3), 308-312, 2009.
[16] N. Muhammad, I. Q. Javed, B. Shahjahan, S. Qurat-ul-Ain, Effect of Medium Composition on Commercially Important Alkaline Protease Production by Bacillus licheniformis N-2". Food Technol. Biotechnol. 46 (4), 388-394, 2008.