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Are PEG Molecules a Universal Protein Repellent?

Authors: Norzita Ngadi, John Abrahamson, Conan Fee, Ken Morison


Poly (ethylene glycol) (PEG) molecules attached to surfaces have shown high potential as a protein repellent due to their flexibility and highly water solubility. A quartz crystal microbalance recording frequency and dissipation changes (QCM-D) has been used to study the adsorption from aqueous solutions, of lysozyme and α-lactalbumin proteins (the last with and without calcium) onto modified stainless steel surfaces. Surfaces were coated with poly(ethylene imine) (PEI) and silicate before grafting on PEG molecules. Protein adsorption was also performed on the bare stainless steel surface as a control. All adsorptions were conducted at 23°C and pH 7.2. The results showed that the presence of PEG molecules significantly reduced the adsorption of lysozyme and α- lactalbumin (with calcium) onto the stainless steel surface. By contrast, and unexpected, PEG molecules enhanced the adsorption of α-lactalbumin (without calcium). It is suggested that the PEG -α- lactalbumin hydrophobic interaction plays a dominant role which leads to protein aggregation at the surface for this latter observation. The findings also lead to the general conclusion that PEG molecules are not a universal protein repellent. PEG-on-PEI surfaces were better at inhibiting the adsorption of lysozyme and α-lactalbumin (with calcium) than with PEG-on-silicate surfaces.

Keywords: Stainless steel, PEG, QCM-D, protein, PEI layer, silicate layer.

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[1] P.Roach, D.Farrar and C.C.Perry, "Interpretation of protein adsorption: surface-induced conformational changes", Journal American Chemical Society, vol. 127, pp. 8168-8173, 2005
[2] F.Zhang, E.T. Kang, K.G. Neoh, P.Wang, K.L. Tan, (2001). Surface modification of stainless steel by grafting of poly (ethylene glycol) for reduction in protein adsorption", Biomaterials, vol. 22, pp. 1541-1548, 2001
[3] J.Wei, D.B. Ravn, L.Gram, and P.Kingshott, "Stainless steel modified with PEG can prevent protein adsorption but not bacterial adhesion", Colloids and Surfaces B: Biointerfaces, vol. 32, pp. 275-291, and 2005
[4] P.Kingshott, W.Jiang, G.B. Nikolaj and G.G. Lone, "Covalent attachment of poly (ethylene glycol) to surfaces, critical forr Bacterial adhesion", Langmuir, vol.19, pp.6912-6921, 2001
[5] J.G. Archambault and J.L. Brash, J.L. "Protein repellent polyurethaneurea surfaces by chemical grafting of hydroxyl-terminated poly (ethylene oxide): effects of protein size and charge", Colloids and Surfaces B: Biointerfaces, vol. 33, pp. 111-120, 2004
[6] A. Roosjen, H.C. Mei, H.J. Busscher, and W. Norde, "Microbial adhesion to poly (ethylene oxide) brushes: influence of polymer chain length and temperature", Langmuir, vol. 20, pp. 10949-10955, 2004
[7] L.D. Unsworth, H. Sheardown and J.L. Brash, "Protein resistance of surfaces prepared by sorption of end-thiolated poly (ethylene glycol) to gold: Effect of surface chain density", Langmuir, vol. 21, pp. 1036- 1041, 2005
[8] J.Zheng, L.Lingyan, H.K.Tsao, Y.J.Sheng, S.Chen and S.Jiang, "Strong repulsive forces between protein and oligo(ethylene glycol) selfassembled monolayers. A molecular simulation study," Biophysical Journal, vol. 89, pp. 158-166, 2005
[9] S.R.Sheth and D.Leckband, "Measuremnet of attractive forces between proteins and end-grafted poly (ethylene glycol) chains", Applied Biological Sciences, vol. 94, pp. 8399-8404, 1997
[10] N.V.Efremova, S.R.Sheth and D.E.Leckband, "Protein -induced changes in Poly (ethylene glycol) brushes: molecular weight and temperature dependence", Langmuir, vol. 17, pp. 7628-7636, 2001
[11] J.J.R Stalgren, J,Eriksson, K.Boschkova, "A comparative study of surfaces using the quartz crystal microbalance and the ellipsometer", Journal of Colloid and Interface Science, vol. 253, pp.190-195, 2002
[12] J.Voros, "The density and refractive index of adsorbing protein layers", Biophysical Journal, vol.87, pp.553-561, 2004
[13] D. Lazos, S. Franzka and M. Ulbricht, "Size selective protein adsorption to polystyrene surfaces by self-assembled grafted poly(ethylene glycols) with varied chain length", Langmuir, vol. 21, pp.8774-8784, 2005
[14] M.Rodahl, F.Hook, C.Fredriksson, C.A. Keller, A.Krozer, P.Brzezinski, M.Voinova and B.Kasemo, "simultaneous frequency and dissipation factor QCM measurements of biomolecular adsorption and cell adhesion", Faraday Discuss, vol. 107, pp. 229-246, 1997
[15] B.S Murray and C. Deshaires, "Monitoring protein fouling of metal surfaces via a quartz crysal microbalance", Journal of Colloidal and Interface Science, vol.227, PP.32-41, 2000
[16] N.Weber, H.Peter, and J.Kohn, "Formation of viscoelastic protein layers on polymeric surfaces relevant to platelet adhesion", Wiley InterScience, pp. 420-427,2005
[17] I.M. Nnebe, R.D. Tilton, J.W. Schneider, "Direct force measurement of the stability of poly(ethylene glycol)-polyethylenimine graft films", Journal of Colloid and Interface Science, vol. 276, pp.306-316, 2004
[18] L.D. Unsworth, H. Sheardown and J.L. Brash, "Protein resistance of surfaces prepared by sorption of end-thiolated poly(ethylene glycol) to gold:Effect of surface chain density", Langmuir, vol. 21, pp.1036-1041, 2005
[19] C.Yoshikawa, A.Goto, Y.Tsujii, T.Fukuda, T.Kimura,K.Yamamoto and A.Kishida, "Protein repellency of well-defined, concentrated poly(2- hydroxyethyl methacrylate) brushes by the size exclusion effect," Macromolecules, pp.2284-2290,2006
[20] I.A. Bardina, N.V. Kovaleva, Yu.S. Nikitin, "Surface properties of initial and polyethylene glycol modified silica gels", Russion Journal of Physical Chemistry, vol. 75, pp. 442-445, March 2001
[21] K.Uchida, H.Otsuka, M.Kaneko, K.Kataoka and Y.Nagasaki, "A reactive poly (ethylene glycol) layer to achieve specific surface plasmon resonance sensing with a high S/N: The substantial role of a short underbrushed PEG layer in minimizing nonspecific adsorption," Analytical Chemistry, vol. 77, pp. 1075-1080, 2005
[22] B.Zhou, T.Eurell, R.Gunawan and D.Leckband, "Chain length dependence of the protein and cell resistance of oligo (ethylene glycol)- terminated self-assembled monolayer-s on gold", Journal of Biomedical Material Research, vol. 56, pp. 406-416, 2001
[23] M.Bjorling, G.Karlstriim and P.Linse., "Conformational Adaption of Poly(ethylene oxide). A 13C-NMR Study", Journal Physical Chemistry, vol. 95, pp. 6706-6709, 1991