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Adhesion Properties of Bifidobacterium Pseudocatenulatum G4 and Bifidobacterium Longum BB536 on HT-29 Human Epithelium Cell Line at Different Times and pH
Abstract:Adhesion to the human intestinal cell is considered as one of the main selection criteria of lactic acid bacteria for probiotic use. The adhesion ability of two Bifidobacteriums strains Bifidobacterium longum BB536 and Bifidobacterium psudocatenulatum G4 was done using HT-29 human epithelium cell line as in vitro study. Four different level of pH were used 5.6, 5.7, 6.6, and 6.8 with four different times 15, 30, 60, and 120 min. Adhesion was quantified by counting the adhering bacteria after Gram staining. The adhesion of B. longum BB536 was higher than B. psudocatenulatum G4. Both species showed significant different in the adhesion properties at the factors tested. The highest adhesion for both Bifidobacterium was observed at 120 min and the low adhesion was in 15 min. The findings of this study will contribute to the introduction of new effective probiotic strain for future utilization.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1333400Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1426
 Bernet M.F., Brassart D., Neeser J. R., and Servin A.L." Lactobacillus acidophilus LA-1 binds to cultured human intestinal cell lines and inhibits cell-attachment and cell-invasion by enterovirulent bacteria," Gut, vol. 35, pp. 483-489., 1994.
 Canzi E., Guglielmetti S., Mora D., Tamagnini I., and Parini, C." Conditions affecting cell surface properties of human intestinal bifidobacteria," Antoine Van Leeuwenhoek, vol.88, pp. 207-219., 2005.
 Collado M., Meriluoto J., and Salminen S." In vitro analysis of probiotic strain combinations to inhibit pathogen adhesion to human intestinal mucus," Food Research International, vol. 40, pp. 629- 636., 2007.
 Del Piano M., Morelli L., et al. "Probiotics: from research to consumer," Digestive and Liver Disease, vol. 2, pp. S248-S255., 2006.
 Ferna'ndez M. S. Boris C. and Barbe'S. "Probiotic properties of human lactobacilli strains to be used in the gastrointestinal tract," Journal of Applied Microbiology, vol. 94, pp. 449-455., 2003.
 Gopal P., Prasad J., Smart J., and Gill H. "In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli," International Journal of Food Microbiology, vol. 67, pp. 207-216., 2001.
 Greene J.D., and Klaenhammer T.R. "Factors involved in adherence of lactobacilli to human Caco-2 cells. Applied and Environmental Microbiology, vol. 60, pp. 4487-4494., 1994.
 Kimoto H., Kurisaki J. et al. " Lactococci as probiotic strains: adhesion to human enterocyte-like Caco-2 cells and tolerance to low pH and bile," Letters in Applied Microbiology, vol. 29, pp. 313-316., 1999.
 Larsen N., Nissen P., and Willats W." The effect of calcium ions on adhesion and competitive exclusion of Lactobacillus ssp. and E. coli O138," International Journal of Food Microbiology, vol. 114, pp. 113- 119., 2007.
 Ouwehand A., Kirjavainen V., Shortt C., and Salminen S. "Probiotics: mechanisms and established effects," International Dairy Journal, vol. 9, pp. 43-52., 1999.
 Parvez S., Malik K.A., Kang S. Ah and Kim H. Y. "Probiotics and their fermented food products are beneficial for health," Journal of Applied Microbiology, vol. 100, pp. 1171-1185., 2006.
 Riedel C., Foata F., Goldstein D., Blum S. and Eikmanns B." Interaction of bifidobacteria with Caco-2 cellÔöÇ adhesion and impact on expression profiles," International Journal of Food Microbiology, vol.110, pp. 62-68., 2005.
 Saarela M., Mogensen G., Fonden R., Matto J.,and Mattila-Sandholm T."Probiotic bacteria: safety, functional and technological properties. Journal of Biotechnology," vol. 84, pp.197-215., 2000.
 Saito Tadao. "Selection of useful probiotic lactic acid bacteria from Lactobacillus acidophilus group and their applications to functional foods," Animal Science Journal, vol. 75, pp.1-13., 2004.
 Schillinge U., Guigas C., and Holzapfel W." In vitro adherence and other properties of lactobacilli used in probiotic yoghurt-like products," International Dairy Journal, vol. 15, pp.1289-1297., 2005.
 Servin A., and Coconnier M. "Adhesion of probiotic strains to the intestinal mucosa and interaction with pathogens," Best Practice and Research Clinical Gastroenterology, vol.5, pp. 741-754., 2003.
 Shuhaimi M., Ali. A.M Norjihan M.S. and Yazid A.M. "Classification of Bifidobactrium isolates from infant faeces using PCR-based and 16S rDNA Partial Sequences Analysis methods," Bioscience Microflora, vol. 21, pp. 155-161., 2002.
 Shuhaimi M., Ali. A.M, Norjihan M.S., Khatijah Y. and Yazid A.M." Differentiation of the Bifidobacterium pseudocatenulatum isolates from faeces of infant by RAPD," Bioscience Microflora, vol. 20, pp. 89-94., 2001.
 Tannock G.W. "Microecology of the gastrointestinal tract in relation to lactic acid bacteria," International Dairy Journal, 5: 1059- 1070., 1995.
 Salminen S., Wright A., and Ouwehand A. "Lactic Acid Bacteria, Marcel Dekker," New York, 2004.
 Tuomola E.M., Ouwehand A.C., and Salminen, S. "Human ileostomy glycoproteins as a model for small intestinal mucus to investigate adhesion of probiotics," Letter of Applied Microbiology, vol. 28, pp. 159-163., 1999.
 Tuomola E.M., and Salminen S.J." Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures," International Journal of Food Microbiol," vol. 41, pp.45-51., 1998.
 Vesterlund S., Paltta J., Karp M., and Ouwehand A." Adhesion of bacteria to resected human colonic tissue: Quantitative analysis of bacterial adhesion and viability," Research in Microbiology, vol. 156, pp. 238-244., 2005.
 Wilson M." Microbial Inhabitants of Humans: Their Ecology and Role in Health and Disease, Cambridge University Press., 2005.
 Modler H.W. "Bifidogenic factorsÔÇösources, metabolism and applications," International Dairy Journa, vol. 4, pp. 383- 407., 1994.
 Jiang T., Mustapha A., and Savaiano D.A." Improvement of lactose digestion in humans by ingestion of unfermented milk containing Bifidobacterium longum," Journal of Dairy Science, vol. 79, pp. 750- 757., 1996.
 Gill H.S. "Stimulation of the immune system by lactic cultures," International Dairy Journal, vol. 8, pp. 535- 544., 1998.
 Gill H. "Probiotics to enhance anti-infective defences in the gastrointestinal tract," Best Practice and Research Clinical Gastroenterology, vol. 17, No. 5, pp. 755-773., 2003.
 Mcnaught C.E. and MacFie J. " Probiotics in clinical practice: a critical review of the evidence," Nutrition Research. 21: 343-353., 2001.
 Gagnon M., Kheadr E. et al. "In vitro inhibition of Escherichia coli O157:H7 by bifidobacterial strains of human origin," International Journal of Food Microbiology, vol. 92, pp. 69-78., 2006.
 Mukai T., Toba T. and H. Ohori. "Collagen binding of Bifidobacterium adolescentis," Current Microbiology, vol. 34, pp. 326-331., 1997.
 Wilson M. "Microbial Inhabitants of Human: Their Ecology and Role in Health and Disease," New York, 2004, pp. 264-265.