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Ionic Liquid Pretreatment and Enzymatic Hydrolysis of Wood Biomass

Authors: F. Peter, M. Ungurean, F. Fitigau, C. Paul, A. Ursoiu


Pretreatment of lignocellulosic biomass materials from poplar, acacia, oak, and fir with different ionic liquids (ILs) containing 1-alkyl-3-methyl-imidazolium cations and various anions has been carried out. The dissolved cellulose from biomass was precipitated by adding anti-solvents into the solution and vigorous stirring. Commercial cellulases Celluclast 1.5L and Accelerase 1000 have been used for hydrolysis of untreated and pretreated lignocellulosic biomass. Among the tested ILs, [Emim]COOCH3 showed the best efficiency, resulting in highest amount of liberated reducing sugars. Pretreatment of lignocellulosic biomass using glycerol-ionic liquids combined pretreatment and dilute acid-ionic liquids combined pretreatment were evaluated and compared with glycerol pretreatment, ionic liquids pretreatment and dilute acid pretreatment.

Keywords: Pretreatment, Enzymatic Hydrolysis, cellulase, lignocellulosicbiomass

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[1] J. B. Binder and R. T. Raines, "Fermentable sugars by chemical hydrolysis of biomass", vol. 107, 2010, pp. 4516-4521.
[2] I. P. Samayam, C. A. Schall, ÔÇ×Saccharification of ionic liquid pretreated biomass with commercial enzyme mixtures", Bioresource Technology, 2010,
[3] pp. 1-6.
[4] M. Zavrel, D. Bross, M. Funke, J. Buchs, A. C. Spiess, "Highthroughput screening for ionic liquids dissolving (ligno-)cellulose", Bioresource Technology, vol. 100, 2009, pp. 2580-2587.
[5] H. Olivier-Bourbigou , L. Magna, D. Morvan, "Ionic liquids and catalysis: Recent progress from knowledge to applications", Applied Catalysis A, vol. 373, 2010, pp. 1-56.
[6] P. Alvira, E. Tomas-Pejo, M. Ballesteros, M.J. Negro, "Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review", Bioresource Technology, 2009, pp. 1- 11.
[7] N. Jain, A. Kumar, S. Chauhan and S. M. S. Chauhan, "Chemical and biochemical transformations in ionic liquids", Tetrahedron, vol. 61, 2005, pp. 1015-1060.
[8] Y. N. Guragain, J. De Coninck, A. Durand, S. K. Rakshit, "Comparison of some new pretreatment methods for second generation bioethanol production from wheat straw and water hyacinth", Bioresource Technology, 2010, pp. 1-36.
[9] S. H. Lee, T. V. Doherty, R. J. Linhardt, J. S. Dordick, "Ionic Liquid- Mediated Selective Extraction of Lignin From Wood Leading to Enhanced Enzymatic Cellulose Hydrolysis", Biotechnology and Bioengineering, vol. 102, 2008, pp. 1368-1375.
[10] Miller, G. L., " Use of dinitrosalicylic acid reagent determination of reducing sugar", Anal. Chem., vol. 31, 1972, pp. 426-428.
[11] T.K Ghose, Measurements of cellulase activities. Pure Appl. Chem. 2, 1987, pp. 257-268.
[12] P. Maki-Arvela, I. Anugwom, P. Virtanen, R. Sjoholm, J.P. Mikkola, "Dissolution of lignocellulosic materials and its constituents using ionic liquidsÔÇöA review", Industrial Crops and Products , vol. 32, 2010, pp. 175-201.
[13] M. Moniruzzaman, K. Nakashima, N. Kamiya, M. Goto, "Recent advances of enzymatic reactions in ionic liquids", Biochemical Engineering Journal, 2009, pp. 1-20.
[14] M. Lucas, G. L. Wagner, Y. Nishiyama, L. Hanson, I. P. Samayam, C. A. Schall, P. Langan, K. D. Rector, "Reversible swelling of the cell wall of poplar biomass by ionic liquid at room temperature", Bioresource Technology, 2011, pp. 1-6.
[15] J. Zhang, X. Ma, J. Yu, X. Zhang, T. Tan, "The effects of four different pretreatments on enzymatic hydrolysis of sweet sorghum bagasse", Bioresource Technology, 2011, pp. 1-5.
[16] C. Li, B. Knierim, C. Manisseri, R. Arora, H. V. Scheller, M. Auer, K. P. Vogel, B. A. Simmons, S. Singh, "Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification", Bioresource Technology, 2009, pp. 1- 7.
[17] H. T. Tan, K. T. Lee, A. R. Mohamed, "Pretreatment of lignocellulosic palm biomass using a solvent-ionic liquid
[BMIM]Cl for glucose recovery: An optimisation study using response surface Methodology", Carbohydrate Polymers, vol. 83, 2011, pp. 1862-1868.