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Microbial Oil Production by Monoculture and Mixed Cultures of Microalgae and Oleaginous Yeasts using Sugarcane Juice as Substrate

Authors: Ratanaporn Leesing, Supaporn Kookkhunthod, Thidarat Papone

Abstract:

Monoculture and mixed cultures of microalgae and the oleaginous yeast for microbial oil productions were investigated using sugarcane juice as carbon substrate. The monoculture of yeast Torulaspora maleeae Y30, Torulaspora globosa YU5/2 grew faster than that of microalgae Chlorella sp. KKU-S2. In monoculture of T. maleeae Y30, a biomass of 8.267g/L with lipid yield of 0.920g/L were obtained, while 8.333g/L of biomass with lipid yield of 1.141g/L were obtained for monoculture of T. globosa YU5/2. A biomass of 1.933g/L with lipid yield of 0.052g/L was found for monoculture of Chlorella sp. KKU-S2. The biomass concentration in the mixed culture of the oleaginous yeast with microalgae increased faster and was higher compared with that in the monocultures. A biomass of 8.733g/L with lipid yield of 1.564g/L was obtained for a mixed culture of T. maleeae Y30 with Chlorella sp. KKU-S2, while 8.010g/L of biomass with lipid yield of 2.424g/L was found for mixed culture of T. globosa YU5/2 with Chlorella sp. KKU-S2. Maximum cell yield coefficient (YX/S, g/L) was found of 0.323 in monoculture of Chlorella sp. KKU-S2 but low level of both specific yield of lipid (YP/X, g lipid/g cells) of 0.027 and volumetric lipid production rate (QP, g/L/d) of 0.003 were observed. While, maximum YP/X (0.303), QP (0.105) and maximum process product yield (YP/S, 0.061) were obtained in mixed culture of T. globosa YU5/2 with Chlorella sp. KKU-S2. The results obtained from the study shows that mixed culture of yeast with microalgae is a desirable cultivation process for microbial oil production.

Keywords: Biodiesel, Mixed Culture, Chlorella sp. KKU-S2, Microbial oil, Torulaspora maleeae Y30, Torulaspora globosa YU5/2

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1332532

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References:


[1] Huang, G.H., Chen, F., Wei, D., Zhang, X.W., and Chen, G. (2010) Biodiesel production by microalgal biotechnology. Appl Energy 87:38- 46.
[2] Chen, C.H., Kuei-Ling Yeh, K.L., Aisyah, R., Lee, D.J., Chang, J.S. (2011) Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review. Bioresour Technol 102: 71- 81.
[3] Brennan, L., Owende, P. (2010) Biofuels from microalgae-a-review of technologies for production, processing, and extractions of biofuels and co-products. Renew Energ Rev 14:557-577.
[4] Mata, T.M., Martins, A.A., Caetano, N.S., (2010) Microalgae for biodiesel production and other applications: a review. Renew Sust Energ Rev 14: 217-232.
[5] Metzger, P., Largeau, C. (2005) Botryococcus braunii: a rich source for hydrocarbons and related ether lipids. Appl Microbiol Biotechnol 66:486-496.
[6] Leesing, R., Nontaso, N. (2010) Microalgal oil production by green microalgae under heterotrophic cultivation. KKU Res J 15 (9): 787-793.
[7] Leesing, R., Kookkhunthod, S. (2011) Heterotrophic growth of Chlorella sp. KKU-S2 for lipid production using molasses as a carbon substrate. Proceedings of the International Conference on Food Engineering and Biotechnology, May 28-29, 2011, Bangkok, Thailand, pp. 87-91.
[8] Papone, T., Kookkhunthod, S., Leesing, R., (2011) Fed-batch heterotrophic and mixotrophic cultivation of Chlorella sp. KKU-S2 for lipid production. Proceedings of the 3rd International Conference on STGMS, March 24-25, 2011, Luang Prabang, Lao PDR. p.84.
[9] Yong-Hong, L., Bo, L., Zong-Bao, Z., Feng-Wu, B., (2006) Optimization of culture conditions for lipid production by Rhodosporidium toruloides. Chinese J Biotechnol 22: 650-656.
[10] Tehlivets, O., Scheuringer, K. and Kohlwein, S.D. (2007). Fatty acid synthesis and elongation in yeast. Biochimica et Biophysica Acta. 1771:255-270.
[11] Leesing, R., Karraphan, P. (2011) Kinetic growth of the isolated oleaginous yeast for lipid production. African J Biotechnol 10 (63): 13867-13877.
[12] Leesing, R., Baojungharn, R. (2011) Microbial Oil Production by Isolated Oleaginous Yeast Torulaspora globosa YU5/2. Proceedings of World Academy of Science, Engineering and Technology 76 2011, Venice, Italy, pp 1088-1092.
[13] O-Reilly, A. M., Scott, J. A. (1995) Defined coimmobilization of mixed microorganism cultures. Enzyme Microb. Technol. 17:636-646.
[14] Pisman, T. I., Somova, L. A. (2003) Interaction of a mixed yeast culture in an "autotroph-heterotroph" system with a closed atmosphere cycle and spatially separated components. Adv. Space Res. 31:1751-1756.
[15] Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-432.
[16] Kwon, D.Y. and Rhee, J.S. (1986) A Simple and rapid colorimetric method for determination of free fatty acids for lipase assay. J Am Oil Chem Soc 63:89-92.
[17] Lee, J.M. (1992) Biochemical Engineering. Prentice Hall international, New Jersey.
[18] Xue, F., Miao, J., Zhang, X., Tan, T. (2010) A new strategy for lipids production by mix cultivation of Spirulina platensis and Rhodotorula glutinis. Appl Biochem Biotechnol 160:498-503.
[19] Richmond, A. (2004) Handbook of microalgal culture: biotechnology and applied phycology. Blackwell Science.
[20] Chiu, S.Y., Kao, C.Y., Chen, C.H., Kuan, T.C., Ong, S.C., Lin, C.S. (2008) Reduction of CO2 by a highdensity culture of Chlorella sp. in a semicontinuous photobioreactor. Bioresour Technol 99:3389-3396.
[21] Ratledge C., Wynn, J.P. (2002) The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. Adv. Appl. Microbiol. 51: 1-51.
[22] Molina Grima E, Fern├índez J, Acién Fern├índez FG, Chisti Y. (2001) Tubular photobioreactor design for algal cultures. J Biotechnol 92:113- 131.
[23] Pope, D.H. (1975) Effects of light intensity, oxygen concentration, and carbon dioxide concentration on photosynthesis in algae. Microb. Ecol. 2:1-16.
[24] Dong, Q.L., Zhao, X.M. (2004) In situ carbon dioxide fixation in the process of natural astaxanthin production by a mixed culture of Haematococcus pluvialis and Phaffia rhodozyma. Catal. Today 98:537- 544
[25] Xiong, W., Li, X., Xiang, J., and Wu, Q. (2008) High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Appl Microbiol Biotechnol 78:29-36.
[26] Zhao, C.H., Zhanga, T., Li, M. and Chia, Z.M. (2010) Single cell oil production from hydrolysates of inulin and extract of tubers of Jerusalem artichoke by Rhodotorula mucilaginosa TJY15a. Process Biochem 45:1121-1126.