Microbial Oil Production by Mixed Culture of Microalgae Chlorella sp. KKU-S2 and Yeast Torulaspora maleeae Y30

Authors: Ratanaporn Leesing, Rattanaporn Baojungharn, Thidarat Papone

Abstract:

Compared to oil production from microorganisms, little work has been performed for mixed culture of microalgae and yeast. In this article it is aimed to show high oil accumulation potential of mixed culture of microalgae Chlorella sp. KKU-S2 and oleaginous yeast Torulaspora maleeae Y30 using sugarcane molasses as substrate. The monoculture of T. maleeae Y30 grew faster than that of microalgae Chlorella sp. KKU-S2. In monoculture of yeast, a biomass of 6.4g/L with specific growth rate (m) of 0.265 (1/d) and lipid yield of 0.466g/L were obtained, while 2.53g/L of biomass with m of 0.133 (1/d) and lipid yield of 0.132g/L were obtained for monoculture of Chlorella sp. KKU-S2. The biomass concentration in the mixed culture of T. maleeae Y30 with Chlorella sp. KKU-S2 increased faster and was higher compared with that in the monoculture and mixed culture of microalgae. In mixed culture of microalgae Chlorella sp. KKU-S2 and C. vulgaris TISTR8580, a biomass of 3.47g/L and lipid yield of 0.123 g/L were obtained. In mixed culture of T. maleeae Y30 with Chlorella sp. KKU-S2, a maximum biomass of 7.33 g/L and lipid yield of 0.808g/L were obtained. Maximum cell yield coefficient (YX/S, 0.229g/L), specific yield of lipid (YP/X, 0.11g lipid/g cells) and volumetric lipid production rate (QP, 0.115 g/L/d) were obtained in mixed culture of yeast and microalgae. Clearly, T. maleeae Y30 and Chlorella sp. KKU-S2 use sugarcane molasses as organic nutrients efficiently in mixed culture under mixotrophic growth. The biomass productivity and lipid yield are notably enhanced in comparison with monoculture.

Keywords: Microbial oil, Chlorella sp. KKU-S2, Chlorella vulgaris, Torulaspora maleeae Y30, mixed culture, biodiesel.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1062978

References:

[1] Huang, G.H., Chen, F., Wei, D., Zhang, X.W., and Chen, G. (2010) Biodiesel production by microalgal biotechnology.Appl Energ 87:38-46.
[2] Chen, C.H., Kuei-Ling Yeh, K.L., Aisyah, R., Lee, D.J., Chang, J.S. (2011) Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production:A critical review. Bioresour Technol 102:71-81.
[3] Mata, T.M., Martins, A.A., Caetano, N.S., (2010) Microalgae for biodiesel production and other applications: a review. Renew Sust Energ Rev 14: 217-232.
[4] Leesing, R., Nontaso, N. (2010) Microalgal oil production by green microalgae under heterotrophic cultivation. KKU Res J 15 (9): 787-793.
[5] Leesing, R., Kookkhunthod, S., Nontaso, N. (2011) Microalgal lipid production by microalgae Chlorella sp. KKU-S2. Proceedings of World Academy of Science, Engineering and Technology, April 27-29, Venice, Italy, pp 441-444.
[6] Yong-Hong, L., Bo, L., Zong-Bao, Z., Feng-Wu, B., (2006) Optimization of culture conditions for lipid production by Rhodosporidium toruloides. Chinese J Biotechnol 22: 650-656.
[7] Leesing, R., Karraphan, P. (2011) Kinetic growth of the isolated oleaginous yeast for lipid production. African J Biotechnol 10 (63): 13867-13877.
[8] Cheng, Y., Lu, Y., Goa, C., Wu, Q. (2009) Algae-based biodiesel production and optimization using sugar cane as the feedstock. Energ Fuels 23:4166-4177.
[9] O-Reilly, A. M., Scott, J. A. (1995) Defined coimmobilization of mixed microorganism cultures. Enzyme Microb Technol 17:636-646.
[10] Lee, J.M. (1992) Biochemical Engineering. Prentice Hall international, New Jersey, pp.138-148.
[11] Kwon, D.Y. and Rhee, J.S. (1986) A Simple and rapid colorimetric method for determination of free fatty acids for lipase assay. J Am Oil Chem Soc 63:89-92.
[12] Xue, F., Miao, J., Zhang, X., Tan, T. (2010) A new strategy for lipids production by mix cultivation of Spirulina platensis and Rhodotorula glutinis. Appl Biochem Biotechnol 160:498-503.
[13] Molina Grima E, Fern├índez J, Acién Fern├índez FG, Chisti Y. (2001) Tubular photobioreactor design for algal cultures. J Biotechnol 92:113- 131.
[14] Pope, D.H. (1975) Effects of light intensity, oxygen concentration, and carbon dioxide concentration on photosynthesis in algae. Microb. Ecol. 2:1-16.
[15] Dong, Q.L., Zhao, X.M. (2004) In situ carbon dioxide fixation in the process of natural astaxanthin production by a mixed culture of Haematococcus pluvialis and Phaffia rhodozyma. Catal. Today 98: 537- 544.
[16] Richmond, A. (1986) Microalgae of economic potential. In Handbook of Microalgal Mass Culture (Richmond, A.,ed.), CRC Press.
[17] Xiong, W., Li, X., Xiang, J., and Wu, Q. (2008) High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Appl Microbiol Biotechnol 78:29-36.