Commenced in January 2007
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Effects of Wastewater Strength and Salt Stress on Microalgal Biomass Production and Lipid Accumulation

Authors: Praepilas Dujjanutat, Pakawadee Kaewkannetra

Abstract:

This work aims to investigate a potential of microalgae for utilizing industrial wastewater as a cheap nutrient for their growth and oil accumulation. Wastewater was collected from the effluent ponds of agro-industrial factories (cassava and ethanol production plants). Only 2 microalgal strains were isolated and identified as Scenedesmus quadricauda and Chlorella sp.. However, only S. quadricauda was selected to cultivate in various wastewater concentrations (10%, 20%, 40%, 60%, 80% and 100%). The highest biomass obtained at 6.6×106 and 6.27×106 cells/ml when 60% wastewater was used in flask and photo-bioreactor. The cultures gave the highest lipid content at 18.58 % and 42.86% in cases of S. quadricauda and S. obliquus. In addition, under salt stress (1.0 M NaCl), S. obliquus demonstrated the highest lipid content at 50% which was much more than the case of no NaCl adding. However, the concentration of NaCl does not affect on lipid accumulation in case of S. quadricauda.

Keywords: cultivation, Microalgae, Cassava wastewater, lipid accumulation

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1062902

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References:


[1] Amin S. Review on biofuel oil and gas production processes from microalgae. Energy Conversion and Management. 2009; 50: 1834-1840.
[2] Ramadhas A.S., Jayaraj S. and Muraleedharan, C. Biodiesel production from high FFA rubber seed oil. Fuel. 84: 335-340.
[3] Dujjanutat, P, Kaewkannetra, P. Potential use of industrial wastewater in selection and cultivation of microalgae as a raw material for biodiesel production Journal of Biotechnology, 2010; 150S: 149-150.
[4] Chinnasamy S., Bhatnagar A., Hant R.W. and Das K.C. Microalgae cultivation in a wastewater dominated by carpet mill effluents logy. 2010; 101: 3097-3105.
[5] Anderson R.A, editor. Algal Culturing Techniques.
[n.p.]. Hing Yip Co; 2005
[6] Burdon K.L. Fatty material in bacteria and funji revealed by staining dried, Fixed slide preparations. Journal of Bacteriology. 52: 665-678.
[7] Iverson S.J., Lang S.L.C. and Cooper M.H. Comparison of Bligh and Dyer and Folch methods for total lipid determination in a broad range of marine tissue. Chemistry and Material Science. 2001; 36(11): 1283- 1287.
[8] Enmak P., Kaewkannetra P. Influences of CO2 concentrations and salinity on acceleration of microalgal oil as raw material for biodiesel production. Journal of Biotechnology. 2010; 150S:19.
[9] Dayananda C., Sarada R., Rani M.U., Shamala T.R. and Ravishankar G.A. Influence of Nitrogen sources on growth, hydrocarbon and fatty acid production by Botryococcus braunii. Asian Journal of plant Sciences. 2006; 5(5): 799-804.
[10] Rattanapoltee, P. Chulalaksananukul, W., James, A.E., Kaewkannetra, P. Comparison of autotrophic and heterotrophic cultivations of microalgae as a raw material for biodiesel production Journal of Biotechnology, 2008; 36: S412
[11] Arroyo T.H., Wei W., Ruan R. and Hu B. Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials. Biomass and Bioenergy. 2011; 35: 2245- 2253.
[12] Hodaifa G., Martínez M.E. and Sánchez S. Use of industrial wastewater from olive-oil extraction for biomass production of Scenedesmus obliquus. Bioresource Technology. 2008; 99: 1111-1117.
[13] Chiu S.Y., Kao C.Y., Chen C.H., Kuan T.C., Ong S.C. and Lin C.S. Reduction of CO2 by a high - density culture of Chlorella sp. in a semicontinuous photo bioreactor. Bioresource Technology. 2008; 99: 3389-3396.
[14] APHA, AWWA, Standard methods of the examination of water and wastewater, 15th edition, Washington, D.C., USA, 1995.
[15] Zhang H., Wang W., Li Y., Yang W. and Shen G. Mixotrophic cultivation of Botryococcus braunii. Biomass and Bioenergy. 2011; 35: 1710-1715.
[16] Kojima E. and Zhang K. Growth and hydrocarbon production of microalga Botryococcus braunii in bubble column photo-bioreactors. Bioscience and Bioenginerring. 1999; 87: 811-815.