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Restriction Specificity of Some Soybean Genotypes to Bradyrhizobium japonicum Serogrous
Abstract:Competitive relationships among Bradyrhizobium japonicum USDA serogroup 123, 122 and 138 were screened versus the standard commercial soybean variety Williams and two introductions P1 377578 "671" in a field trial. Displacement of strain 123 by an effective strain should improved N2 fixation. Root nodules were collected and strain occupancy percentage was determined using strain specific fluorescent antibodies technique. As anticipated the strain USDA 123 dominated 92% of nodules due to the high affinity between the host and the symbiont. This dominance was consistent and not changed materially either by inoculation practice or by introducing new strainan. The interrelationship between the genotype Williams and serogroup 122 & 138 was found very weak although the cell density of the strain in the rhizosphere area was equal. On the other hand, the nodule occupancy of genotypes 671 and 166 with rhizobia serogroup 123 was almost diminished to zero. . The data further exhibited that the genotypes P1 671 and P1 166 have high affinity to colonize with strains 122 and 138 whereas Williams was highly promiscuous to strain 123.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1062044Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 993
 Amerger, N and Lobreau, J.P. (1982). Quantitive study of nodulation competitiveness in Rhizobium strains. Appl. Environ. Microbiol. 44: 583 - 588.
 Ham, G.E. (1980). Ineractions of Glycine max. and Rhizobium japonicum. In.R.J. Summerfield and A.H. Bunting (ed.) Advances in Legume Science. Royal Botinical Gardens. Kew, UK.: 289 - 296.
 Cregan, B.E. and van Berkum, P. (1984). Genetics of nitrogen metabolism and physiological / biochemical selection for increased grain crop productivity. Theor. Appl. Genet. 67: 97 - 111.
 Lohrke, S,M.; Orf, J.H.; Martinez-Romero, E. and Sadowsky, M.J. (1995). Host control restriction of nodulation by Bradyrhizobium japonicum strains in serogroup 110. Appl. Environ. Microbiol., 61 (6): 2378 - 2383.
 Ham, G.E.; Frederick, L.R. and Anderson, I.C. (1971). Serogroups of Rhizobium japonicum in soybean nodules. Agron. J. 63: 69 - 72.
 Kvien, C.S.; Ham, J.E. and Lambert, J.W. (1981). Recovery of introduced Rhizobium japonicum strains by soybean genotypes. Agron J. 73: 900 - 905.
 Moawad, H.A.; Ellis, W.R. and Schmidt, E.L. (1984). Rhizosphere response as a factor of competition among three serogroups of indigenious Rhizobium japonicum for nodulation of field grown soybeans. Appl. Environ. Microbiol. 47: 607 - 612.
 Ham, G.E. (1976). Competition among strains of rhizobia. In. I.D. Hill (ed.). World Soybean Research. Institute Printers and Puplishers, Danville,IL.
 Cregan, P.B. and Keyser, H.H. (1986). Host restrication of nodulation by Bradyrhizobium japonicum strain USDA 123 in soybean. Crop Science, 26: 911 - 916.
 Weiser, G.C.; Skipper, H.D. and Wollum, A.G. 11 (1990). Exclusion of inefficient Bradyrhizobium japonicum serogroups by soybean genotypes. Plant and Soil, 121 (1): 99 - 105.
 Ferrey, M.L.; Graham, P.H. and Russelle, M.P. (1994). Nodulation efficiency of Bradyrhizobium japonicum strains with genotypes of soybean varying in the ability to restrict nodulation. Canadian Journal of Microbiolgy, 40 (6): 456 - 460.
 Schmidt, E.L.; Bankole, R.O. and Bohlool, B.B. (1968). Fluorscentantibody approach to study rhizobia in soil. J. Bacteriol. 95: 1987 - 1992.
 Vincent, J.M. (1970). A Manual for The Practical Study of The Rootnodule Bacteria. Int. Biological Programme Handbook no. 15. Blackwell Scientific Puplication, Oxford, UK.
 Reyes, V.G. and Schmidt,E.L. (1981). Population of Rhizobium japonicum associated with the surfaces of soil grown roots. Plant and Soil, 61: 71 - 80.
 Pazdernik, D.L.; Vance, C.P.; Sadowsky, M.J.; Graham, P.H. and Orf, J.H. (1997). A host- controlled, serogroup- specific, ineffectivenodulation system in the Bradyrhizobium-soybean (Glycine max) symbiosis. Molecular-Plant-Microbe-Interaction, 10 (8): 994 -1001.
 Caldwell, B.E. and Vest, G. (1970). Effect of Rhizobium japonicum strains on soybean yields. Crop. Sci. 10: 19 - 21.
 Cregan P.B.; Keyser, H.H. and Sadowsky, M.J. (1989). Host plant effects on nodulation and competitiveness of the Bradyrhizobium japonicum serotype strains consisting serocluster 123. Appl. Environ. Microbiol., 55 (10): 2532 - 2536.