Integrated Cultivation Technique for Microbial Lipid Production by Photosynthetic Microalgae and Locally Oleaginous Yeast

Authors: Mutiyaporn Puangbut, Ratanaporn Leesing

Abstract:

The objective of this research is to study of microbial lipid production by locally photosynthetic microalgae and oleaginous yeast via integrated cultivation technique using CO2 emissions from yeast fermentation. A maximum specific growth rate of Chlorella sp. KKU-S2 of 0.284 (1/d) was obtained under an integrated cultivation and a maximum lipid yield of 1.339g/L was found after cultivation for 5 days, while 0.969g/L of lipid yield was obtained after day 6 of cultivation time by using CO2 from air. A high value of volumetric lipid production rate (QP, 0.223 g/L/d), specific product yield (YP/X, 0.194), volumetric cell mass production rate (QX, 1.153 g/L/d) were found by using ambient air CO2 coupled with CO2 emissions from yeast fermentation. Overall lipid yield of 8.33 g/L was obtained (1.339 g/L of Chlorella sp. KKU-S2 and 7.06g/L of T. maleeae Y30) while low lipid yield of 0.969g/L was found using non-integrated cultivation technique. To our knowledge this is the unique report about the lipid production from locally microalgae Chlorella sp. KKU-S2 and yeast T. maleeae Y30 in an integrated technique to improve the biomass and lipid yield by using CO2 emissions from yeast fermentation.

Keywords: Microbial lipid, Chlorella sp. KKU-S2, Torulaspora maleeae Y30, oleaginous yeast, biodiesel, CO2 emissions

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1328710

References:

[1] Chisti, Y. (2007) Biodiesel from microalgae. Biotechnol Adv 25: 294-306.
[2] Amin, S. (2009) Review on biofuel oil and gas production processes from microalgae. Energy Conversion and Management 50:1834-1840.
[3] Mata, T.M., Martins, A.A., Caetano, N.S., (2010) Microalgae for biodiesel production and other applications: a review. Renew Sust Energ Rev 14: 217-232.
[4] Huang, G.H., Chen, F., Wei, D., Zhang, X.W., and Chen, G. (2010) Biodiesel production by microalgal biotechnology. Appl Energy 87:38-46.
[5] Powell, E.E., Hill, G.A. (2009) Economic assessment of an integrated bioethanol-biodiesel-microbial fuel cell facility utilizing yeast and photosynthetic algae. Chem Eng Res Design 87: 1340-1348.
[6] Leesing, R., Nontaso, N. (2010) Microalgal oil production by green microalgae under heterotrophic cultivation. KKU Res J 15 (9): 787-793.
[7] Yong-Hong, L., Bo, L., Zong-Bao, Z., Feng-Wu, B., (2006) Optimization of culture conditions for lipid production by Rhodosporidium toruloides. Chinese J Biotechnol 22: 650-656.
[8] Zhu, L.Y., Zong, M.H. and Wu, H. (2008) Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation. Biores Technol 99:7881-7885.
[9] Leesing, R., Karraphan, P. (2011) Kinetic growth of the isolated oleaginous yeast for lipid production. African J Biotechnol 10 (63): 13867-13877.
[10] Kwon, D.Y. and Rhee, J.S. (1986) A Simple and rapid colorimetric method for determination of free fatty acids for lipase assay. J Am Oil Chem Soc 63:89-92.
[11] Lee, J.M. (1992) Biochemical Engineering. Prentice Hall international, New Jersey, pp.138-148.
[12] Wood A.M., Everroad R.C., Wingard L.M. (2005) Measuring growth rates in microalgal cultures. In: Andersen RA, editor. Algal culturing techniques. Elsevier Academic Press. p. 269-285.
[13] Richmond, A. (2004) Handbook of microalgal culture: biotechnology and applied phycology. Blackwell Science.
[14] Chiu, S.Y., Kao, C.Y., Chen, C.H., Kuan, T.C., Ong, S.C., Lin, C.S. (2008) Reduction of CO2 by a highdensity culture of Chlorella sp. in a semicontinuous photobioreactor. Bioresour Technol 99:3389–3396.
[15] Ratledge C., Wynn, J.P. (2002) The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. Adv. Appl. Microbiol. 51: 1-51.
[16] Tehlivets, O., Scheuringer, K., Kohlwein, S.D. (2007) Fatty acid synthesis and elongation in yeast. Biochimica et Biophysica Acta, 1771: 255-270.