Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30075
Differentiation between Common Tick Species Using Molecular Biology Techniques in Saudi Arabia

Authors: Kholoud A. Al-Shammery , Badr El-Sabah A. Fetoh, Ahmed M. Alshammari

Abstract:

Protein and Esterase electrophoresis were used to genetically identify two Saudi tick species. Engorged females of the camel tick Hyalomma dromedarii (Koch) (Acari: Ixodidae) and the cattle tick Boophilus annulatus (Say) (Acari: Ixodidae) ticks collected from infested camels and cattle in the animals resting house at Hail region in KSA were used. The results showed that there are a variation in both of protein and esterase activity levels and a high polymorphism within and between the genera and species of Hyalomma and Boophilus . In conclusion, the protein and esterase electrophoretic analysis used in the present study could successfully distinguish among tick species, commonly found in Saudi Arabia.

Keywords: Molecular biology, The camel tick Hyalomma dromedarii, The cattle tick Boophilus annulatus , Ticks.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1061667

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2405

References:


[1] J. R. Mohler, "Tick Fever," Agri. Farmer-s Bull., 1990, vol. 1625, pp 1- 30
[2] K. L. Poucher, H. J. Hutcheson, J. E. Keirans, L. A. Durden, and W. C. Black, "IV. Molecular genetic key for the identification of 17 Ixodes species of the United States (Acari: Ixodidae): A methods model", J. Parasito., 1999, vol. 85(4), pp. 623-629.
[3] K. M. El Kammah, and M. A. Sayed, "Biochemical fingerprints of egg and salivary gland proteins characterising four common tick genera in Egypt", 1999.
[4] K. L. Poucher, H. J. Hutcheson, J. E. Keirans, L. A. Durden, and W. C. Black, "Molecular genetic key for the identification of 17 Ixodes species of the United States (Acari: Ixodidae): a methods model". J. Parasitol., vol. 85, 1999, pp. 623-629.
[5] B. E. A. Fetoh, "Molecular and modern taxonomy of Dacus spp. (Diptera: Tephritidae) attacking vegetables in Egypt", J. Egypt. Ger. Soc. Zool., 2006, vol. 48E, pp. 1-21.
[6] O. H. Lowry, C. S. Rosebrough, and R. J. Randall, " Protein measurement with the folin phenol reagent-, J. Biolo. Chem., 1951, vol. 193, pp. 265-275.
[7] U. K. Laemmli, "Cleavage of structural proteins during assembly of head bacteriophage T4 " ", Nature, 1970, vol. 227, pp. 680- 685.
[8] P. J. Hitchcock, and T. M. Brown, "Morphological heterogeneity among Salmomella lipopolysaccaride types in Silver - stained poly acrylamide gel", J. Bact., 1983, vol. 154, pp. 269-277.
[9] Sims, M. "Methods for detection of enzymatic activity after electrophoresis on polyacrylamide gel in Drosophila species" Nature, 1965, vol. 207(14), pp. 757-758.
[10] M. Nei, and W.H. Li, "Mathematical model for studying genetic variation in terms of restriction endonucleases", Proc. Natl. Acad. Sci. (USA), 1979, vol. 76, pp. 5269-5273.
[11] Y. Yorozu, M. Hirano, K. Oka, and Y. Tagawa, "Electron spectroscopy studies on magneto-optical media and plastic substrate interfaces(Translation Journals style)," IEEE Transl. J. Magn.Jpn., vol. 2, Aug. 1987, pp. 740-741 (Dig. 9th Annu. Conf. Magnetics Japan, 1982, p. 301).
[12] D.S. Hayer, and D. McInnis, "Resolution of populations of the Mediterranean fruit fly Ceratitis capitata at DNA level using random primers)," Genome, 1994, vol. 37, pp. 244-248.
[13] Tatchell, R.J. Electrophoretic studies on the proteins of the haemolymph, saliva, and eggs of the cattle tick, Boophilus microplus . Insect Biochem., 1971, vol.1,no. 1, pp. 45-55.
[14] C. Delaye, L. Beati, A. Aeschimann, F. Renaud, and T. de Meeus, "Population genetic structure of Ixodes ricinus in Switzerland from allozymic data: no evidence of divergence between nearby sites", Int. J. Parasitol., 1997, vol. 27, pp. 769-773.
[15] Gh. Norouzi, R. Hashemitabar, and G. R. Razmi, "Detection of midgut antigens of Hyalomma anatolicum anatolicum tick using SDS-PAGE and Western Blot", Iranian Journal of Veterinary Research, Shiraz University, 2007, vol. 8 (2), pp. 166-169.
[16] A. M. Avanzati, M. Baratti, and F. Bernini," Molecular and morphological differentiation between steganacarid mites (Acari:Oribatida)", Biol. J. Linn. Soc., 1994, vol. 52, pp. 25-340.
[17] D. E. Kain, F. A. Sperling, and R.S. Lane, " Population genetic structure of Ixodes pacificus (Acari: Ixodidae) using allozymes", J. Med. Entomol., 1997, vol. 34, pp. 441-450.
[18] Z.A .El-Fiky, M.A. Sayed and K.M. El Kammah , "Esterase and RAPD-PCR analysis of common tick species in Egypt", Arab J. Biotech. 2003,,vol. 6, no.1, pp. 39-48.