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Effect of Plasma Therapy on Epidermal Regeneration

Authors: Magda Bădescu, Daniela Jitaru, C.Grigoraş, L.Bădescu, I. Topala, Manuela Ciocoiu


The purpose of our study was to compare spontaneous re-epithelisation characteristics versus assisted re-epithelisation. In order to assess re-epithelisation of the injured skin, we have imagined and designed a burn wound model on Wistar rat skin. Our aim was to create standardised, easy reproducible and quantifiable skin lesions involving entire epidermis and superficial dermis. We then have applied the above mentioned therapeutic strategies to compare regeneration of epidermis and dermis, local and systemic parameter changes in different conditions. We have enhanced the reepithelisation process under a moist atmosphere of a polyurethane wound dress modified with helium non-thermal plasma, and with the aid of direct cold-plasma treatment respectively. We have followed systemic parameters change: hematologic and biochemical parameters, and local features: oxidative stress markers and histology of skin in the above mentioned conditions. Re-epithelisation is just a part of the skin regeneration process, which recruits cellular components, with the aid of epidermal and dermal interaction via signal molecules.

Keywords: Plasma medicine, re-epithelisation and tissue regeneration

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[1] Alvarez, O.M,, Mertz PM, Eaglestein WH. The effect of occlusive dressings on collagen synthesis and re-epithelialization in superficial wounds. J Surg Res 35: 142-8; 1983.
[2] Arturson, G. Pathophysiology of the burn wound. Ann Chir Gyn; 69: 178; 1980.
[3] Bothwell, J.W., Rovee DT, Downes AM, Flanagan PA, Kurowsky CA. The effects of the climate on the repair of cutaneous wounds in humans. In: Maibach HI, Rovee DT, eds. Epidermal wound healing. Chicago: Year Book Medical Publishers, Inc., 255-66; 1972.
[4] Cross, K.J., Mustoe TA - Growth Factors in Wound Healing, Surg Clin N Am, 83 531-545; 2003.
[5] Eaglestein, W.H., Davis SC, Mchlc AL, Mertz PM. Optimal use of an occlusive dressing to enhance healing. Arch Dermatol, 1988.
[6] Friedman, G., Peddinghaus M, Fridman A, Balasubramanian M, Gutsol A, Friedman G. Use of Non-Thermal Atmospheric Pressure Plasma Discharge for Coagulation and Steriliztion of Surface Wounds, Plasma Chemistry and Plasma Processing, 2006.
[7] Friedman, G; Chirokov, et al., Non-Thermal Atmospheric Pressure Discharges, Journal of Physics D-Applied Physics, 2005.
[8] Hermans, M.H.E., Hermans RP. Duoderm, an alternative dressing for smaller burns. Burns 1986; 12: 214-9;
[9] Krawcyk, WS., A pattern of epidermal cell migration during wound healing. J Cell Biol; 49: 247-63. 99; 1971.
[10] May, S.R., Physiology, immunology, and clinical efficacy of an adherent polyurethane wound dressing: Opsitc, Wise DL, cd. Burn wound coverings. Boca Raton: CRC Press, Inc., 53-78; 1984.
[11] May, S.R., Properties of an adherent polyurcthanc wound dressing, Care of the burn wound. Basel: Kargcr, 204-17, 1985.
[12] Miller, T.A., The healing of partial-thickness skin injuries. In: Hunt KH,ed. Wound healing and wound infection. New York: Appleton- Century-Crofts, 81-96; 1980.
[13] Petrie, N.C., Feng Yao, Eriksson E - Gene Therapy in Wound Healing, Surg Clin N Am, 83, 597-616; 2003.
[14] Schwentker, A., Timothy, R., - Billiar-Nitric Oxide and Wound Repair, Surg Clin N Am 83, 521-530; 2003.
[15] Sosnin, Stoffels, et al., The Effects of Uv Irradiation and Gas Plasma Treatment on Living Mammalian Cells and Bacteria: A comparatice Approach, Ieee Transactions on Plasma Science, 2004.
[16] Winter, G.D., A note on wound healing under dressings with special reference to perforated-film dressings. J Invest Dermatol; 45: 299-302; 1965.
[17] Winter, G.D., Formation of the scab and the rate of cpithclization of superficial wounds in the skin of the young domestic pig. Nature (Lond); 193: 293-4; 1962.
[18] Winter, G.D., Healing of skin wounds and the influence of dressings on the repair process. In: Harkiss KJ, ed. Surgical dressings and wound healing. Bradford: Bradford University Press, 46-60; 1971.
[19] Zawacki, B.E., Reversal of capillary statis and prevention of necrosis in burns. Ann Surg; 180: 98-102; 1974.
[20] Ebell, B., The Papyrus Ebers. The greatest Egyptian medical document. Coopenhagen: Levin and Munlsgaard, 1937.
[21] Stickel, D.L., Seigler HF. Transplantation. I. Historical aspects. In: Sabiston DC, Textbook of Surgery, 11th ed. Philadelphia: WB Saunders Company, 4:56-63, 1977.
[22] Vasilets, V.N., A. Gutsol, A.B. Shekhter, A. Fridman. Plasma Medicine.
[23] High Energy Chemistry, v.43 (N3) pp.229-233, 2009.
[24] Gregory Fridman, Victor N.Vasilets, Alexander Gutsol, Gary Friedman, Anatoly B.Shekhter, Alexander Fridman. Applied Plasma Medicine. Plasma Processes and Polymers, N5, pp.503-533, 2008.
[25] Yildirim Eda D., Halim Ayan, Victor N.Vasilets, Alexander Fridman, Selcuk Guceri, Wei Sun. Effect of Dielectric Barrier Discharge Plasma on the Attachment And Proliferation of Osteoblasts Cultured over Poly (e-Caprolactone) Scaffolds.