The Influence of Surface Potential on the Kinetics of Bovine Serum Albumin Adsorption on a Biomedical Grade 316LVM Stainless Steel Surface

Authors: Khawtar Hasan Ahmed, Sasha Omanovic

Abstract:

Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) in combination with electrochemistry, was employed to study the influence of surface charge (potential) on the kinetics of bovine serum albumin (BSA) adsorption on a biomedical-grade 316LVM stainless steel surface is discussed. The BSA adsorption kinetics was found to greatly depend on the surface potential. With an increase in surface potential towards more negative values, both the BSA initial adsorption rate and the equilibrium (saturated) surface concentration also increased. Both effects were explained on the basis of replacement of well-ordered water molecules at the 316LVM / solution interface, i.e. by the increase in entropy of the system.

Keywords: adsorption, biomedical grade stainless steel, bovine serum albumin (BSA), electrode surface potential / charge, kinetics, PM-IRRAS, protein/surface interactions

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1060479

References:

[1] Pasche S, Voros J, Griesser HJ, Spencer ND, Textor M. Effects of ionic strength and surface charge on protein adsorption at PEGylated surfaces. Journal of Physical Chemistry B 2005;109: 17545-52.
[2] Martins MCL, Wang D, Ji J, Feng L, Barbosa MA. Albumin and fibrinogen adsorption on PU-PHEMA surfaces. Biomaterials 2003;24: 2067-76.
[3] Moulton SE, Barisci JN, Bath A, Stella R, Wallace GG. Investigation of protein adsorption and electrochemical behavior at a gold electrode. Journal of Colloid and Interface Science 2003;261: 312-9.
[4] Trojanowicz M, Krawczyk TKV. Electrochemical Biosensors Based on Enzymes Immobilized in Electropolymerized Films. Mikrochimica Acta 1995;121: 167-81.
[5] Cornelius RM, Wojciechowski PW, Brash JL. Measurement of Protein Adsorption-Kinetics by An Insitu, Real-Time, Solution Depletion Technique. Journal of Colloid and Interface Science 1992;150: 121-33.
[6] Fukuzaki S, Urano H, Nagata K. Adsorption of Protein Onto Stainless- Steel Surfaces. Journal of Fermentation and Bioengineering 1995;80: 6- 11.
[7] Noh H, Vogler EA. Volumetric interpretation of protein adsorption: Competition from mixtures and the Vroman effect. Biomaterials 2007;28: 405-22.
[8] Weber N, Wendel HP, Ziemer G. Hemocompatibility of heparin-coated surfaces and the role of selective plasma protein adsorption. Biomaterials 2002;23: 429-39.
[9] Burns NL, Holmberg K, Brink C. Influence of Surface Charge on Protein Adsorption at An Amphoteric Surface - Effects of Varying Acid to Base Ratio. Journal of Colloid and Interface Science 1996;178: 116-22.
[10] Bentaleb A, Abele A, Haikel Y, Schaaf P, Voegel JC. FTIR-ATR and radiolabeling study of structural modifications during protein adsorption on hydrophilic surfaces. 2. The Case of apo-alpha-lactalbumine. Langmuir 1999;15: 4930-3.
[11] Price ME, Cornelius RM, Brash JL. Protein adsorption to polyethylene glycol modified liposomes from fibrinogen solution and from plasma. Biochimica et Biophysica Acta - Biomembranes 2001;1512: 191-205.
[12] Seitz R, Brings R, Geiger R. Protein adsorption on solid-liquid interfaces monitored by laser-ellipsometry. Applied Surface Science 2005;252: 154- 7.
[13] Hook F, Voros J, Rodahl M, Kurrat R, Boni P, Ramsden JJ et al. A comparative study of protein adsorption on titanium oxide surfaces using in situ ellipsometry, optical waveguide lightmode spectroscopy, and quartz crystal microbalance/dissipation. Colloids and Surfaces BBiointerfaces 2002;24: 155-70.
[14] Armstrong J, Salacinski HJ, Mu QS, Seifalian AM, Peel L, Freeman N et al. Interfacial adsorption of fibrinogen and its inhibition by RGD peptide: a combined physical study. Journal of Physics-Condensed Matter 2004;16: S2483-S91.
[15] Toscano A, Santore MM. Fibrinogen adsorption on three silica-based surfaces: Conformation and kinetics. Langmuir 2006;22: 2588-97.
[16] Ngankam AP, Mao GZ, Van Tassel PR. Fibronectin adsorption onto polyelectrolyte multilayer films. Langmuir 2004;20: 3362-70.
[17] Wegner GJ, Wark AW, Lee HJ, Codner E, Saeki T, Fang SP et al. Realtime surface plasmon resonance imaging measurements for the multiplexed determination of protein adsorption/desorption kinetics and surface enzymatic reactions on peptide microarrays. Analytical Chemistry 2004;76: 5677-84.
[18] Evans-Nguyen KM, Fuierer RR, Fitchett BD, Tolles LR, Conboy JC, Schoenfish MH. Changes in adsorbed fibrinogen upon conversion to fibrin Langmuir 2006;22: 5115-21.
[19] Forciniti D, Hamilton WA. Surface enrichment of proteins at quartz/water interfaces: A neutron reflectivity study. Journal of Colloid and Interface Science 2005;285: 458-68.
[20] Petrash S, Cregger T, Zhao B, Pokidysheva E, Foster MD, Brittain WJ et al. Changes in protein adsorption on self-assembled monolayers with monolayer order: Comparison of human serum albumin and human gamma globulin. Langmuir 2001;17: 7645-51.
[21] Cosman NP, Roscoe SG. Electrochemical quartz crystal nanobalance (EQCN) studies of protein interfacial behavior at Pt. Langmuir 2004;20: 1711-20.
[22] Wright JEI, Cosman NP, Fatih K, Omanovic S, Roscoe SG. Electrochemical impedance spectroscopy and quartz crystal nanobalance (EQCN) studies of insulin adsorption on Pt. Journal of Electroanalytical Chemistry 2004;564: 185-97.
[23] Hemmersam AG, Foss M, Chevallier J, Besenbacher F. Adsorption of fibrinogen on tantalum oxide, titanium oxide and gold studied by the QCM-D technique. Colloids and Surfaces B-Biointerfaces 2005;43: 208- 15.
[24] Martins MCL, Fonseca C, Barbosa MA, Ratner BD. Albumin adsorption on alkanethiols self-assembled monolayers on gold electrodes studied by chronopotentiometry. Biomaterials 2003;24: 3697-706.
[25] Ithurbide A, Frateur I, Galtayries A, Marcus P. XPS and flow-cell EQCM study of albumin adsorption on passivated chromium surfaces: Influence of potential and pH. Electrochimica Acta 2007;53: 1336-45.
[26] Wagner MS, Castner DG. Analysis of adsorbed proteins by static timeof- flight secondary ion mass spectrometry. Applied Surface Science 2004;231-2: 366-76.
[27] Ademovic Z, Klee D, Kingshott P, Kaufmann R, Hocker H. Minimization of protein adsorption on poly(vinylidene fluoride). Biomolecular Engineering 2002;19: 177-82.
[28] Li LY, Chen SF, Jiang SY. Protein adsorption on alkanethiolate selfassembled monolayers: Nanoscale surface structural and chemical effects. Langmuir 2003;19: 2974-82.
[29] Tunc S, Maitz MF, Steiner G, Vazquez L, Pham MT, Salzer R. In situ conformational analysis of fibrinogen adsorbed on Si surfaces. Colloids and Surfaces B-Biointerfaces 2005;42: 219-25.
[30] Rodrigues SN, Goncalves IC, Martins MCL, Barbosa MA, Ratner BD. Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers. Biomaterials 2006;27: 5357-67.
[31] Song L, Meng J, Zhong J, Liu LF, Dou XY, Liu DF et al. Human fibrinogen adsorption onto single-walled carbon nanotube films. Colloids and Surfaces B-Biointerfaces 2006;49: 66-70.
[32] Schwinte P, Voegel JC, Picart C, Haikel Y, Schaaf P, Szalontai B. Stabilizing effects of various polyelectrolyte multilayer films on the structure of adsorbed/embeded fibrinogen molecules: An ATR-FTIR study. Journal of Physical Chemistry B 2001;105: 11906-16.
[33] Roach P, Farrar D, Perry CC. Interpretation of protein adsorption: Surface-induced conformational changes. Journal of the American Chemical Society 2005;127: 8168-73.
[34] Frateur I, Lartundo-Rojas L, Methivier C, Galtayries A, Marcus P. Influence of bovine serum albumin in sulphuric acid aqueous solution on the corrosion and the passivation of an iron-chromium alloy. Electrochimica Acta 2006;51: 1550-7.
[35] Desroches MJ, Chaudhary N, Omanovic S. PM-IRRAS investigation of the interaction of serum albumin and fibrinogen with a biomedical-grade stainless steel 316LVM surface. Biomacromolecules 2007;8: 2836-44.
[36] Omanovic S, Roscoe SG. Electrochemical studies of the adsorption behavior of bovine serum albumin on stainless steel. Langmuir 1999;15: 8315-21.
[37] Jackson DR, Omanovic S, Roscoe SG. Electrochemical studies of the adsorption behavior of serum proteins on titanium. Langmuir 2000;16: 5449-57.
[38] Cabilio NR, Omanovic S, Roscoe SG. Electrochemical studies of the effect of temperature and pH on the adsorption of alpha-lactalbumin at Pt. Langmuir 2000;16: 8480-8.
[39] Cosman NP, Fatih K, Roscoe SG. Electrochemical impedance spectroscopy study of the adsorption behaviour of alpha-lactalbumin and beta-casein at stainless steel. Journal of Electroanalytical Chemistry 2005;574: 261-71.
[40] Omanovic S, Roscoe SG. Interactive adsorption behavior of betalactoglobulin and linoleate at a 316L stainless steel surface. Electrochemical and Solid State Letters 2005;8: E12-E5.
[41] Omanovic S, Roscoe SG. Interfacial behavior of beta-lactoglobulin at a stainless steel surface: An electrochemical impedance spectroscopy study. Journal of Colloid and Interface Science 2000;227: 452-60.
[42] Kwok KC, Yeung KM, Cheung NH. Adsorption kinetics of bovine serum albumin on fused silica: Population heterogeneities revealed by singlemolecule fluorescence microscopy. Langmuir 2007;23: 1948-52.
[43] Desroches MJ, Omanovic S. Adsorption of fibrinogen on a biomedicalgrade stainless steel 316LVM surface: a PM-IRRAS study of the adsorption thermodynamics, kinetics and secondary structure changes. Physical Chemistry Chemical Physics 2008;10: 2502-12.
[44] Wahlgren M, Arnebrant T. Protein Adsorption to Solid-Surfaces. Trends in Biotechnology 1991;9: 201-8.
[45] Maruyama T, Katoh S, Nakajima M, Nabetani H, Abbott TP, Shono A, et al. FT-IR analysis of BSA fouled on ultrafiltration and microfiltration membranes. Journal of Membrane Science 2001;192: 201-7.
[46] McClellan SJ, Franses EI. Adsorption of bovine serum albumin at solid/aqueous interfaces. Colloids and Surfaces A-Physicochemical and Engineering Aspects 2005;260: 265-75.
[47] Sukhishvili SA, Granick S. Adsorption of human serum albumin: Dependence on molecular architecture of the oppositely charged surface. Journal of Chemical Physics 1999;110: 10153-61.
[48] Wertz CF, Santore MM. Adsorption and relaxation kinetics of albumin and fibrinogen on hydrophobic surfaces: Single-species and competitive behavior. Langmuir 1999;15: 8884-94.
[49] Zeng HT, Chittur KK, Lacefield WR. Analysis of bovine serum albumin adsorption on calcium phosphate and titanium surfaces. Biomaterials 1999;20: 377-84.
[50] Bernabeu P, Caprani A. Influence of surface charge on adsorption of fibrinogen and/or albumin on a rotating disc electrode of platinum and carbon. Biomaterials 1990;11: 258-64.
[51] Bos MA, Shervani Z, Anusiem ACI, Giesbers M, Norde W, Kleijn JM. Influence of the electric potential of the interface on the adsorption of proteins. Colloids Surfaces B: Biointerfaces 1994;3: 91-100.
[52] Asanov AN, Delucas LJ, Oldham PB, Wilson WW. Heteroenergetics of Bovine Serum Albumin Adsorption from Good Solvents Related to Crystallization Conditions. Journal of Colloid and Interface Science 1997;191: 222-35.
[53] Brewer SH, Glomm WR, Johnson MC, Knag MK, Franzen S. Probing BSA binding to citrate-coated gold nanoparticles and surfaces. Langmuir 2005;21: 9303-7.
[54] Silin V, Weetall H, Vanderah DJ. SPR studies of the nonspecific adsorption kinetics of human IgG and BSA on gold surfaces modified by self-assembled monolayers (SAMs). Journal of Colloid and Interface Science 1997;185: 94-103.
[55] Tidwell CD, Ertel SI, Ratner BD, Tarasevich BJ, Atre S, Allara DL. Endothelial Cell Growth and Protein Adsorption on Terminally Functionalized, Self-Assembled Monolayers of Alkanethiolates on Gold. Langmuir 1997;13: 3404-13.
[56] Dent AH, Aslam M. Other categories of protein coupling. Bioconjugation: Protein Coupling Techniques for the Biomedical Sciences. Edited by Aslam M, Dent AH. London: Macmillan Reference; 1998: 504-69.
[57] Yin G, Liu Z, Zhan J, Ding FX, Yuan NJ. Impacts of the surface charge property on protein adsorption on hydroxyapatite. Chemical Engineering Journal 2002;87: 181-6.
[58] Rezwan K, Studart AR, Voros J, Gauckler LJ. Change of ╬ potential of biocompatible colloidal oxide particles upon adsorption of bovine serum albumin and lysozyme. Journal of Physical Chemistry B 2005;109: 14469-74.
[59] Shahryari A. Nhancement of Biocompatibility of 316LVM Stainless Steel by Electrochemical Cyclic Potentiodynamic Passivation. Ph.D. Thesis. McGill University; 2008.
[60] Pyshnov E, Farcas M, Cosman N, Roscoe SG, Omanovic S. The Cell Biomaterial Reaction. European Cells and Materials Journal 2004;7(suppl. 1): 80.
[61] Desroches MJ. Electrochemical and PM-IRRAS studies of the interaction of plasma protein fibrinogen with a biomedical-grade 316LVM stainless steel surface. Master. Thesis. McGill University; 2007.
[62] Nadarajah A, Lu CF, Chittur KK. Modeling the Dynamics of Protein Adsorption to Surfaces. Proteins at Interfaces II: Fundamentals and Applicactions. Edited by Horbett TA, Brash JL. Washington, D.C: American Chemical society; 1995: 181-94.
[63] Norde W. Adsorption of Proteins from Solution at the Solid-Liquid Interface. Advances in Colloid and Interface Science 1986;25: 267-340.
[64] Liu CI, Hsu KY, Ruaan RC. Hydrophobic contribution of amino acids in peptides measured by hydrophobic interaction chromatography. Journal of Physical Chemistry B 2006;110: 9148-54.
[65] Damian A, Omanovic S. Ni and Ni-Mo hydrogen evolution electrocatalysts electrodeposited in a polyaniline matrix. Journal of Power Sources 2006;158: 464-76.
[66] Navarro-Flores E, Chong ZW, Omanovic S. Characterization of Ni, NiMo, NiW and NiFe electroactive coatings as electrocatalysts for hydrogen evolution in an acidic medium. Journal of Molecular Catalysis A-Chemical 2005;226: 179-97.
[67] Navarro-Flores E, Omanovic S. Hydrogen evolution on nickel incorporated in three-dimensional conducting polymer layers. Journal of Molecular Catalysis A-Chemical 2005;242: 182-94.