Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31324
Quantification of Peptides based on Isotope Dilution Surface Enhanced Raman Scattering

Authors: F. Yaghobian, R. Stosch, B. Güttler

Abstract:

This study aims to demonstrate the quantification of peptides based on isotope dilution surface enhanced Raman scattering (IDSERS). SERS spectra of phenylalanine (Phe), leucine (Leu) and two peptide sequences TGQIFK (T13) and YSFLQNPQTSLCFSESIPTPSNR (T6) as part of the 22-kDa human growth hormone (hGH) were obtained on Ag-nanoparticle covered substrates. On the basis of the dominant Phe and Leu vibrational modes, precise partial least squares (PLS) prediction models were built enabling the determination of unknown T13 and T6 concentrations. Detection of hGH in its physiological concentration in order to investigate the possibility of protein quantification has been achieved.

Keywords: Quantification, peptides, surface enhanced Raman scattering

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1060167

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1358

References:


[1] L.Seballos, N. Richards, D. J. Stevens, M. Patel, L. Kapitzky, S. Lokey, G. Millhauser, J. Z. Zhang, Chemical Physics Letters, 2007, 447, 335- 339
[2] D.R.J. Bainbridge, S.A. Ellis, I.L. Sargent, J. Reprod. Immunol. 2000, 48, 17.
[3] N. Andreasen, L. Minthon, P. Davidsson, E. Vanmechelen, H. Vanderstichele, B. Winblad, K. Blennow, Arch. Neurol. 58, 2001, 373.
[4] M.A. Albert, E. Danielson, N. Rifai, P.M. Ridker, JAMA 286, 2001, 64.
[5] P. Argani et al., Clin. Cancer Res. 7, 2001, 3862.
[6] K. Ryu, A. J. Haes, H. Park, S. Nah, J. Kim, H. Chung, M. Yoon, S. Han, J. Raman Spectroscopy, 2010, 41, 2, 121-124
[7] S. M. Hewitt, J. Dear, R. A. Star, J. Am. Soc. Nephrol. 2004, 15, 1677.
[8] S. Bi, H. Zhou, S. Zhang, Biosens. Bioelectron. 2009, 24, 2961.
[9] K. A. Jellinger, B. Janetzky, J. Attems, E. Kienzl, J.Cell.Mol.Med. 2008, 12(4), 1094.
[10] F. Wei, D. Zhang, N. J. Halas, and J. D. Hartgerink, J. Phys. Chem. B, 2008, 112, 9158-9164
[11] T. Vo-Dinh, D.L. Stokes, G.D. Griffin, M. Volkan, U.J. Kim, M.I. Simon, J. Raman Spectrosc. 1999, 30, 785.
[12] V. Brun, C. Masselon, J. Garin, A. Dupuis, J. Proteomics, 2009, 72, 740-749.
[13] C. G. Arsene, R. Ohlendorf, W. Burkitt, C. Pritchard, A. Henrion, G. O'Conner, D. M. Bunk, and B. G├╝ttler, Anaytical Chemistry, 2008, 80, 4154-4160
[14] M. D. Porter, R. J. Lipert, L. M. Siperko, G.Wang, R.Narayanana, Chem. Soc. Rev. 2008, 37, 1001.
[15] W. E. Doering, M. E. Piotti, M. J. Natan, R. G. Freeman, Adv. Mater. 2007, 19, 3100.
[16] R. Stosch, F. Yaghobian, T. Weimann, R. J. C. Brown, M. J. T. Milton and B. G├╝ttler, Nanotechnology, 2011, 22, 105303.
[17] S. Stewart, P. M. Fredericks, Spectrochim. Acta, Part A 1999, 55, 1641.
[18] J. S. Suh, M. Moskovits, J. Am. Chem. Soc. 1986, 108, 4711.
[19] T. M. Herne, A. Ahern, R. L. Garrell, J. Am. Chem. Soc. 1991,113, 846.
[20] E. Podstawka, Y. Ozaki, L.M. Proniewicz, Appl. Spectrosc. 2005,59, 1516.
[21] H. I. Lee, S. W. Suh, M.S. Kim, J. Raman Spectrosc. 1988, 19,491.
[22] C. D. Keating, K.M. Kovaleski, M. J. Natan, J. Phys. Chem. B 1998, 102, 9404.
[23] L. A. Gearheart, H. J. Ploehn, C. J. Murphy, J. Phys. Chem. B 2001, 105, 12609.
[24] R. Stosch, A. Henrion, D. Schiel, B. G├╝ttler, Anaytical Chemistry, 2005, 77, 7386-7392.
[25] F. Yaghobian, T. Weimann, B. G├╝ttler, R. Stosch, Lab on a Chip, 2011, 11, 2955-2960.
[26] F. Yaghobian, IDSERS based Quantification of Biomarkers on a Single Chip; Mench & Buch, Berlin, 2011.
[27] T. Tsushima, Y. Katoh, Y. Miyachi, K. Chihara, A. Teramoto, M. Irie, Y. Hashimoto, The Journal of Clinical Endocrinology & Metabolism, 1999, Vol. 84, No. 1 317-322.
[28] C. Ortiz, D. Zhang, Y. Xie, V.J. Davisson, D. Ben-Amotz, Anal. Biochem. 2004, 332, 245.