Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30172
Genetic Polymorphism of Main Lactoproteins of Romanian Grey Steppe Breed in Preservation

Authors: Şt. Creangâ, V. Maciuc, A.V. Bâlteanu, S.S. Chelmu

Abstract:

The paper presents a part of the results obtained in a complex research project on Romanian Grey Steppe breed, owner of some remarkable qualities such as hardiness, longevity, adaptability, special resistance to ban weather and diseases and included in the genetic fund (G.D. no. 822/2008.) from Romania. Following the researches effectuated, we identified alleles of six loci, codifying the six types of major milk proteins: alpha-casein S1 (α S1-cz); beta-casein (β-cz); kappa-casein (K-cz); beta-lactoglobulin (β-lg); alpha-lactalbumin (α-la) and alpha-casein S2 (α S2-cz). In system αS1-cz allele αs1-Cn B has the highest frequency (0.700), in system β-cz allele β-Cn A2 ( 0.550 ), in system K-cz allele k-CnA2 ( 0.583 ) and heterozygote genotype AB ( 0.416 ) and BB (0.375), in system β-lg allele β-lgA1 has the highest frequency (0.542 ) and heterozygote genotype AB ( 0.500 ), in system α-la there is monomorphism for allele α-la B and similarly in system αS2-cz for allele αs2-Cn A. The milk analysis by the isoelectric focalization technique (I.E.F.) allowed the identification of a new allele for locus αS1-casein, for two of the individuals under analysis, namely allele called αS1-casein IRV. When experiments were repeated, we noticed that this is not a proteolysis band and it really was a new allele that has not been registered in the specialized literature so far. We identified two heterozygote individuals, carriers of this allele, namely: BIRV and CIRV. This discovery is extremely important if focus is laid on the national genetic patrimony.

Keywords: allele, breed, genetic preservation, lactoproteins, Romanian Grey Steppe

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1059567

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1326

References:


[1] ┼×t. Creangâ., V. Maciuc, M. P├«ntea : Polimorfismul genetic ├«n ameliorarea bovinelor din zona Moldovei. Editura ÔÇ×Ion Ionescu de la Brad", Ia┼ƒi, 2008, pp. 23 - 65.
[2] V. Maciuc, ┼×t. Creangâ, M. P├«ntea : Ameliorarea geneticâ a bovinelor ├«n regiunrea transfrontalierâ. Editura ÔÇ×Ion Ionescu de la Brad", Ia┼ƒi, 2008, pp. 166 - 175.
[3] I. Hekken, s. a. : Functional properties of dephosphorylated bovine whole casein., J. Dairy Science, nr. 11, 1993.
[4] C., J. Mercier, s. a. : Génétique moléculaire des protéines du lait et de leurs gènes. Biologie de la lactation. ├ëditions INRA., 1993.
[5] B. Ribadeau-Dumas : Protèines du lait i structure et fonctions. Biologie de la lactation. ├ëdition INRA, 1993.
[6] ┼×t. Creangâ, V. Maciuc, M. P├«ntea, I. G├«lcâ, R. Danaila : Romanian Grey Steppe cattle breed in the context of genetic resources preservation in Romania. 3-rd International Symposium, 18-20 September, University of Technology and Life Sciences in Bydgoszcz, Poland. Book of abstract. Journal Central European of Agriculture, 2008, pp. 34 - 35.
[7] V. Râileanu : Sisteme polimorfe lactoproteice ┼ƒi influenţa lor asupra proprietâţilor tehnologice ale laptelui. Tezâ de doctorat, U.A.S.M. Chi┼ƒinâu, Republica Moldova, 2002.
[8] A., V. Balteanu, A. Vlaic, F., D. Pop, A., R. Rusu s.a. : Characteriyation at protein level of the new ╬▒S1-cazein allele IRV discovered in Romanian Grey Steppe cattle breed Moldavian variety. Bulletin of USAMV - BT Anim. Sci. 4, 2008, pp. 1-10.
[9] A., V. Balteanu, A. Vlaic, A., R. Rusu, ┼×t. Creangâ, s.a. : Milk proteins polymorphism in Romanian cattle breeds, identified by isoelectric focusing technique (IEF). Bulletin of USAMV Iasi, Anim. Sci. 50, 2007, pp.173-181.
[10] F. Grosclaude ┼ƒi colab. : A mendelian polymorphism underlyng quantitative variations of goat ╬▒S1-casein. Génét. Sél. Evol., 4, 1987, pp. 399 - 412.
[11] F. Grosclaude : Le polymorphisme génétique des principales lactoprotéines bovines. INRA, Prod. Anim., 1, 1988, pp. 5 - 17.
[12] Ng-Kwai-Hang s. a. : Relationshisps between milk protein polymorphisms and major milk constituants in Holstein-Friesian cows. J. Dairy Sci., 69, 1986, pp. 22-26.
[13] F., M. Mahé, G. Miranda, R., Queval, A. Bado, P.S. Zafindrajaona, and F. Grosclaude : Genetic polymorphism of milk proteins in African Bos taurus and Bos indicus populations. Characterization of variants aS1- Cn H and k-Cn J. Genet. Sel. Evol. 31: 239 - 253, 1999.
[14] Y., Kawamoto, T.A. Namikawa, T. Adachi, T. Amano, T. Shotake, T. Nishida, Y. Hayashi, B. Kattel, and H.B. Rajubhandary : A population genetic study on yaks, cattle and their hybrids in Nepal using milk protein variations. Anim. Sci. Tech. 63: 563 - 575, 1992.