Rapid Determination of Biochemical Oxygen Demand

Authors: Mayur Milan Kale, Indu Mehrotra

Abstract:

Biochemical Oxygen Demand (BOD) is a measure of the oxygen used in bacteria mediated oxidation of organic substances in water and wastewater. Theoretically an infinite time is required for complete biochemical oxidation of organic matter, but the measurement is made over 5-days at 20 0C or 3-days at 27 0C test period with or without dilution. Researchers have worked to further reduce the time of measurement. The objective of this paper is to review advancement made in BOD measurement primarily to minimize the time and negate the measurement difficulties. Survey of literature review in four such techniques namely BOD-BARTTM, Biosensors, Ferricyanidemediated approach, luminous bacterial immobilized chip method. Basic principle, method of determination, data validation and their advantage and disadvantages have been incorporated of each of the methods. In the BOD-BARTTM method the time lag is calculated for the system to change from oxidative to reductive state. BIOSENSORS are the biological sensing element with a transducer which produces a signal proportional to the analyte concentration. Microbial species has its metabolic deficiencies. Co-immobilization of bacteria using sol-gel biosensor increases the range of substrate. In ferricyanidemediated approach, ferricyanide has been used as e-acceptor instead of oxygen. In Luminous bacterial cells-immobilized chip method, bacterial bioluminescence which is caused by lux genes was observed. Physiological responses is measured and correlated to BOD due to reduction or emission. There is a scope to further probe into the rapid estimation of BOD.

Keywords: BOD, Four methods, Rapid estimation

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1332438

References:

[1] Sawyer C., McCarty P., Parkin G. (2006)"Chemistry for Environmental Engineering and Sciences", Tata McGraw-Hill Publishing Company, fifth edition.
[2] Logan, B.E. and Wagenseller G.A.(1993). The HBOD test: a new method for determining biochemical oxygen demand. Water Environ. Res. (65-7), p: 862-868.
[3] APHA, Standard Methods for the Examination of Waters and Wastewater. 20th Edn.(2005). American Public Health Association, Washington, DC.
[4] Cullimore, R., (1999), Biological Activity Reaction Tests- A simple yet effective method for monitoring Biochemical Oxygen Demand.BARTTM testers. Practical Atlas for Bacerial Identification, Lewis/CRC Press, Droycon Bioconcepts Inc. 315 Dewdney Ave. Regina, Saskatchewan, Canada S4N 0E7.
[5] Johnston L., Cullimore R and Singh K.,. (1990), Field Trials of the BOD-BART system™ for the Rapid Determination of Biochemical Oxygen Demand in Secondary and Tertiary Effluents. Droycon Bioconcepts Inc, Regina, Saskatchewan, and University of New Brunswick, Fredricton, New Brunswick.
[6] Karube, I., Matsunaga, T., Mitsuda, S., Suzuki, S., (1977.), Microbial Biosensors Biotechnol. Bioeng. (19), p.1535-1547.
[7] Rastogi S, Rathee P, Saxena T.K., Mehra N.K., Kumar R.(2002) BOD analysis of industrial effluents: 5 days to 5 min, Current Applied Physics (3),p-191-194.
[8] Hikuma M., Suzuki, H., Yasuda, T., Karube, I., Suzuki, S., (1979). Amperometric estimation of BOD by using living immobilized yeast, Eur. J. Appl. Microbiol. Biotechnol. (8), 289-297.
[9] Ohki A, Shinohara K., Lto O., Naka K., Maeda S., Sato T., Akano H., Kato N., Kawamura Y., (1994) ,A BOD sensor using Klebsiella Oxytoca ASI, Int. J. Environ. Anal. Chem. (56).p261-269.
[10] Jung J., Sofer S., Lakhwala F., (1995) Towards an on-line biochemical oxygen demand analyser, Biotechnol. Tech.(9) 289-294.
[11] Sangeetha S, Sugandhi G., Murugesan M., Madhav V.M., Berchmans S., Rajasekar R., Rajasekar S., D., Jeyakumar G., Rao P., (1996), Immobilised Activated Sludge Based Biosensor For Biochemical Oxygen Demand Measurement, Electroanalysis (8),p: 698-701.
[12] Jianbo Jia, Tang Mingyu, Xu Chen, Li Qi, Shaojun Dong. (2002), Coimmobilized microbial biosensor for BOD estimation based on sol-gel derived composite material, Biosensors and Bioelectronics (18), p- 1023-/1029.
[13] Dong, S., Cheng, G., Li, B., Kou, W., Zhang, J., (1996). Construction of enzyme electrodes based on PVA-g-PVP hydrogel. Chin. Pat. CN96123528.4.
[14] Kylie Catterall , Kristy Morris, Huijun Zhao, Neil Pasco, Richard John. (2001), The use of microorganisms with broad range substrate utilization for the ferricyanide-mediated rapid determination of biochemical oxygen demand, Talanta (55),p-1187-1194.
[15] Toshifumi Sakaguchi , Yasunori Morioka , Masahiro Yamasaki , Junpei Iwanaga , Kazuhiko Beppu, Hideaki Maeda, Yasutaka Morita , Eiichi Tamiya. (2006), Rapid and onsite BOD sensing system using luminous bacterial cells-immobilized chip, Biosensors and Bioelectronics (19),p- 115-121.