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Pollution Induced Community Tolerance(PICT) of Microorganisms in Soil Incubated with Different Levels of PB

Authors: N. Aliasgharzad, A. Molaei, S. Oustan

Abstract:

Soil microbial activity is adversely affected by pollutants such as heavy metals, antibiotics and pesticides. Organic amendments including sewage sludge, municipal compost and vermicompost are recently used to improve soil structure and fertility. But, these materials contain heavy metals including Pb, Cd, Zn, Ni and Cu that are toxic to soil microorganisms and may lead to occurrence of more tolerant microbes. Among these, Pb is the most abundant and has more negative effect on soil microbial ecology. In this study, Pb levels of 0, 100, 200, 300, 400 and 500 mg Pb [as Pb(NO3)2] per kg soil were added to the pots containing 2 kg of a loamy soil and incubated for 6 months at 25°C with soil moisture of - 0.3 MPa. Dehydrogenase activity of soil as a measure of microbial activity was determined on 15, 30, 90 and 180 days after incubation. Triphenyl tetrazolium chloride (TTC) was used as an electron acceptor in this assay. PICTs (€IC50 values) were calculated for each Pb level and incubation time. Soil microbial activity was decreased by increasing Pb level during 30 days of incubation but the induced tolerance appeared on day 90 and thereafter. During 90 to 180 days of incubation, the PICT was gradually developed by increasing Pb level up to 200 mg kg-1, but the rate of enhancement was steeper at higher concentrations.

Keywords: Induced tolerance, soil microorganisms, Pb, PICT, pollutants.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1332260

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References:


[1] S.R. Smith, "Agricultural Recycling of Sewage Sludge in the Environment" CABI Biosciences, Wallingford, UK, 1996.
[2] K.E. Giller, E. Witter, and S.P. McGrath, "Toxicity of heavy metals to microorganisms and microbial process in agricultural soils: a review" Soil Biol. Biochem. vol. 300, pp.1389-1414, 1998.
[3] P.C. Brookes, "The potential of microbiological properties as indicators in soil-pollution monitoring" in Soil Monitoring. Early Detection and Surveying of Soil Contamination and Degradation, R. Schulin, A. Desaules, R. Webster, and B. von Steiger, Eds. Birkha¨user Verlag, Basel, Switzerland, 1993, pp 229-254.
[4] S.P. MacGrath, "Effects of heavy metals from sewage sludge on soil microbes in agricultural ecosystems" in Toxic metals in soil-plant systems, S.M. Ross, Ed., John Wiley, Chichester, 1994, pp. 242-274
[5] S.P. MacGrath, A.M. Chaudri, and K.E. Giller, "Longterm effects of metals in sewage sludge on soils, microorganisms and plants" J. Ind. Microbiol. Vol.14, pp.94-104, 1995.
[6] K. Chander, and P.C. Brookes, "Effects of heavy metals from past applications on microbial biomass and organic matter accumulation in a sandy loam U.K. soil" Soil Biol. Biochem. Vol.23, pp. 927-932, 1991.
[7] K. Chander, and P.C. Brookes, "Residual effects of zinc, copper and nickel in sewage sludge on microbial biomass in a sandy loam" Soil Biol. Biochem,vol. 25, pp.1231-1239, 1993.
[8] B. Olson, and I. Thornton, "The resistance patterns to metals of bacterial populations in contaminated land" J Soil Sci. vol.33, pp.271-277, 1982.
[9] M. Diaz-Ravina, and E. Bååth, "Development of metal tolerance in soil bacterial communities exposed to experimentally increased metal levels" Appl Environ Microbiol. Vol.62, pp.2970-2977, 1996.
[10] M. Diaz-Ravina, and E. Bååth, "Response of soil bacterial communities pre-exposed to different metals and reinoculated in an unpolluted soil" Soil Biol Biochem. Vol.33, pp.241-248, 2001.
[11] H. Blanck, S.A. Wangberg, and S. Molander, "Pollution-induced community tolerance. A new ecotoxicological tool" in Functional Testing of Aquatic Biota for Estimating Hazards of Chemicals STP 988. J.J. Cairns, and J.R. Pratt, Eds. American Society for Testing, Philadelphia, PA, 1988, pp. 219-230.
[12] P. Van Beelen, A.K. Fleuren-Kemila, and T. Aldenberg, "The relation between extrapolated risk, expressed as potentially affected fraction, and community effects, expressed as pollution-induced community tolerance" Environ Toxicol Chem. Vol.20, pp.1133-1140, 2001.
[13] M.E.Y. Boivin, A.M. Breure, L. Posthuma, and M. Rutgers, "Determination of field effects of contaminants-significance of pollution- induced community tolerance" Human and Ecological Risk Assessment, vol. 8, pp.1035-1055,2002.
[14] K. Lock, and C.R. Janssen, "Influence of soil zinc concentrations on zinc sensitivity and functional diversity of microbial communities" Environ. Pollut. Vol.136, pp.275-281, 2005.
[15] H. Bayer, M. Mitterer, and F. Schinner, "Der Ein.uss von Insektiziden auf mikrobiogene Prozesse in Ah-Materialien eines landwirtschaftlich genutzten Bodens" Pedobiologia, vol. 23, pp.311-319, 1982.
[16] R.P. Dick, "Soil enzyme activities as integrative indicators of soil health" in Biological indicators of soil health,C.E. Pankhurst, B.M. Doube, and V.V.S.R. Gupta, Eds. Wallingford, USA, CAB International, 1997, pp. 121-156.
[17] E.M. Top, M.P. Maila, M. Clerinx, J. Goris, P. De Vos, and W. Verstraete, "Methane oxidation as method to evaluate the removal of 2,4-D from soil by plasmid-mediated bioaugumentation" FEMS Microbiol. Ecol. Vol.28, pp.203-213,1999.
[18] L. Gianfreda, M.A. Rao, A. Piotrowska, G. Palumbo, and C. Colombo, "Soil enzyme activities as affected by anthropogenic alterations: intensive agricultural practices and organic pollution" Sci. Total Environ. Vol.341, pp. 265-279, 2005.
[19] P. Doelman, and L. Haanstra, "Short and long term effects of heavy metals on urease activity in soils" Biol. Fertil. Soils. Vol.2, pp. 213-218, 1894.
[20] C. Marzadori, C. Ciavatta, D. Montecchio, and C. Gessa, "Effetcs of lead pollution on different soil enzyme activities" Biol. Fertil. Soils. Vol.22, pp.53-58, 1996.
[21] S.P. Mathur, J.I. MacDougall, and M. McGrath, "Levels of activities of some carbohydrates, protease, lipase and phosphates in organic soils of differing copper content" Soil Science. Vol.129, pp.376-385, 1980.
[22] G. Tyler, "Heavy metal pollution and soil enzyme activity" Plant and Soil. Vol.41, pp.303-311, 1974.
[23] M.A. Tabatabai, "Effects of trace elements on urease activity" Soil Biol. Biochem. vol.9, pp.9-13, 1977.
[24] M.B. Hinojosa, J.A. Carreira, R. García-Ruíz, and R.P. Dick, "Soil moisture pre-treatment effects on enzyme activities as indicators of heavy metal-contaminated and reclaim soils" Soil Biol. Biochem. vol.30, pp.1559-1568, 2004.
[25] P. Nannipieri, "The potential use of soil enzymes as indicators of productivity, sustainability and pollution" in Soil Biota, Management in Sustainable Farming Systems, C.E. Pankhurst, B.M. Doube, ,V.V.S.R. Gupta, and P.R.Grace, Eds. Australia, CSIRO Publication, 1994, pp.238-244.
[26] C. Trasar-Cepeda, M.C. Leir├│s, S. Seoane, and F. Gil-Sotres, "Limitations of soil enzymes as indicators of soil pollution" Soil Biol. Biochem. vol.32, pp.1867-1875, 2000.
[27] J.H. Bolton, Elliot, L.F. R.I. Papendick, and D.F. Berdicek, "Soil microbial biomass and selected soil enzyme activities: effects of fertilization and cropping practices" Soil Biol. Biochem. Vol.14, pp. 423-427, 1985.
[28] G. Shen, Y. Lu, Q. Zhou, and J. Hong, "Interaction of polycyclic aromatic hydrocarbons and heavy metals on soil enzyme" Chemosphere. Vol.61, pp.1175-1182, 2005.
[29] A.L. Page, "Methods of Soil Analysis" Part 2, Chemical and Microbiological Properties. Second edition. Agronomy number 9. U.S.A, 1982.
[30] M. Rutgers, and A.M. Breure, "Risk assessment, microbial communities and pollution-induced community tolerance" Human and Ecol Risk Assessment. Vol.5, pp.661-670, 1998.
[31] C.O. Nweke, C. Ntinugwa, I.F. Obach, S.C. Ike, G.E. Eme, E.C. Opara, J.C. Okolo, and C.E. Nwanyanwu, "In vitro effects of metals and pesticides on dehydrogenase activity in microbial community of cowpea (Vigna unguiculata) rhizoplane" African J. Biotech. Vol.6, pp.290-295, 2007.
[32] L. Alden Demoling, and E. Bååth, "Use of pollution-induced community tolerance of the bacterial community to detect phenol toxicity in soil" Environ. Toxicol. Chem. Vol.27, pp. 344-350, 2008.
[33] L.S. Zeng, M. Liao, C.L. Chen, and C.Y. Huang, "Effects of lead contamination on soil enzymatic activities, microbial biomass and rice physiological indices in soil-lead-rice (Oryza sativa L.) system. Ecotoxicol" Environ. Safety. Vol.67, pp.67-74, 2007.
[34] K. Saeki, T. Kunito, H. Oyaizu, and S. Matsumoto, "Relationships between bacterial tolerance levels and forms of copper and zinc in soils" J. Environ. Qual. Vol.31, pp.1570-1575, 2002.
[35] A.R. Almas, L.R. Bakken, and J. Mulder, " Changes in tolerance of soil microbial communities in Zn and Cd contaminated soils" Soil Biol. Biochem. Vol.36, pp.805-813, 2004.
[36] M. Diaz-Ravina, R. Calvo de Anta, and E. Bååth, "Tolerance (PICT) of the bacterial communities to copper in Vineyards soils from Spain" J. Environ. Qual. Vol.36, pp.1760-1764, 2007.
[37] M. Niklinska, M.Chodak, and R. Laskowski, "Pollution-induced community tolerance of microorganisms from forest soil organic layers polluted with Zn or Cu" Appl Soil Ecol. Vol. 32, pp.265-272, 2006.
[38] K. Kaiser, and G. Guggenberger, "The role of DOM sorption to mineral surfaces in the preservation of organic matter in soils" Org. Geochem. Vol.31, pp.711-725, 2000.
[39] Y. Wang, J. Shi, Q. Lin, X. Chen, and Y. Chen, "Heavy metal availability and impact on activity of soil microorganisms along a Cu/Zn contamination gradient" J. Environ Sci. vol.19, pp.848-853, 2007.
[40] J.J. Kelly, M. Häggblom, and R.L. Tate ΙΙΙ, "Changes in soil microbial communities over time resulting from one time application of zinc: a laboratory microcosm study" Soil Biol. Biochem. vol.31, pp.1455-1465, 1999.
[41] M. Liao, L. Yun-kuo, Z. Xiao-min, and H. Chang-yong, "Toxicity of cadmium to soil microbial biomass and its activity: Effect of incubation time on Cd ecological dose in paddy soil" J. Zhejiang University Science. Vol.5, pp.324-330, 2005.
[42] E. Kandeler, D. Tscherko, K.D. Bruce, M. Stemmer, P.J. Hobbs, R.D. Bardgett, and W. Amelung, "Structure and function of the soil microbial community in microhabitats of a heavy metal polluted soil" Biol. Fertil Soil. Vol.32, pp.390-400, 2000.
[43] T.W. Speir, H.A. Kettles, A. Parshotam, P.L. Searle, and L.N.C. Vlaar, "A simple kinetic approach to derive the ecological dose value, ED50, for the assessment of Cr (VI) toxicity to soil biological properties" Soil Biol. Biochem. vol.27, pp.801-810,1995.
[44] L. Alden Demoling, and E. Bååth, "No long-term persistence of bacterial pollution-induced community tolerance in tylosin-polluted soil" Environ. Sci. Tecchnol. Vol.42, pp. 6917-6921, 2008.
[45] M. Diaz-Ravina, and E. Bååth, "Influence of different temperatures on metal tolerance measurements and growth response of bacterial communities from unpolluted and polluted soils" Biol. Fertil. Soils. Vol.21, pp. 233-238, 1996.
[46] T. Duxbury, "Ecological aspects of heavy metal responses in microorganisms" in Advances in Microbial Ecology, K.C. Marshall, Ed. vol. 8. Plenum, New York, 1985, pp. 185-236.
[47] L.Posthuma, "Effects of toxicants on population and community parameters in field conditions, and their potential use in the validation of risk assessment methods" in Ecological risk assessment of contaminats in soil, N.M. Van Straalen., and H. Lokke, Eds. Chapman and Hall, London, 1997, pp. 85-117.