Extraction and Characterisation of Protein Fraction from Date Palm Fruit Seeds
Authors: Ibrahim A. Akasha, Lydia Campbell, Stephen R. Euston
Abstract:
Date palm (Phoenix dactylifera L.) seeds are waste streams which are considered a major problem to the food industry. They contain potentially useful protein (10-15% of the whole date-s weight). Global production, industrialisation and utilisation of dates are increasing steadily. The worldwide production of date palm fruit has increased from 1.8 million tons in 1961 to 6.9 million tons in 2005, thus from the global production of dates are almost 800.000 tonnes of date palm seeds are not currently used [1]. The current study was carried out to convert the date palm seeds into useful protein powder. Compositional analysis showed that the seeds were rich in protein and fat 5.64 and 8.14% respectively. We used several laboratory scale methods to extract proteins from seed to produce a high protein powder. These methods included simple acid or alkali extraction, with or without ultrafiltration and phenol trichloroacetic acid with acetone precipitation (Ph/TCA method). The highest protein content powder (68%) was obtained by Ph/TCA method with yield of material (44%) whereas; the use of just alkali extraction gave the lowest protein content of 8%, and a yield of 32%.
Keywords: Date palm seed, Phoenix dactylifera L., extraction of date palm seed protein
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1330285
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[1] FAO stat data base, 2007, www.FAO.org.
[2] Hamada, J.S., Hashim, I.B., and Sharif, F.A. Preliminary analysis and potential uses of date pits in foods. Food chemistry, 76, pp.135-137, 2002.
[3] Official methods of analysis, 15edition: Association of Official Analytical Chemists, AOAC, Washington, D.C, USA, 1995.
[4] Onsaard, E.; Pomsamud, P. and Audtum, P. Functional properties of sesame protein concentrates from sesame mel As.J.Ag-Ind. 3(04), pp.420-431, 2010.
[5] Wang, H.; Johnson, L. A. and Wang, T. Preparation of soy protein concentration and isolate from extruded-expel led soybean meals. Journal of the American oil chemists society, 81, pp.713-717, 2004.
[6] L-hocine, B. Composition and functional properties of soy protein isolates prepared using alternative defatting and extraction procedures. Journal of food science, 71, pp.137-145, 2006.
[7] Bouaziz, M., Besbes, S., Blecker, C., Wathelet, B., Deroanne, C., and Hamadi, A. Protein and amino acid profiles of Tunisian Deglet Nour and Allig date palm fruit seeds. Fruits, 63, pp.37-43, 2008.
[8] Wang, W.; Scali, M.; Vignani, R., Spadaford, A.Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high leavls of interfering compounds. Electrophoresis 24, pp. 2369 - 2375, 2003.
[9] Vidal, S. G.; Tena, M.; Liorca, L. V. L. and Salinas, J. Protein extraction from Phoenix dactylifera L. Leaves, a recalcitrant material for twodimensional electrophoresis. Electrophoresis, 29, pp.448-456, 2008.
[10] Al-Hooti, S.; Sidhu, J.S. and Qabazard, H. Physicochemical characteristics of five date fruit cultivars grown in the United Arab Emirates. Plant Foods for Human Nutrition, 50, pp.101-113, 1997.
[11] Al-Showiman, S.S., & Baosman, A.A. Protein and amino acid contents of some Saudi Arabian date palm seeds (Phenix dactylifera L.) Arab Gulf J. Scient. Res., 10(2), 1-9, 1992.
[12] Al-Hooti, S.; Sidhu, J. S. and Qabazard, H.Chemical composition of seeds of date fruit cultivars of United Arab Emirates. J. Food Sci. Technol., 35(1), pp.44-46,1998.
[13] Al-Shurfa, M. Y.; Ahmed, H. S. and Abou-Naji, S. E.Organic and inorganic constituents of date palm pit(seed). Date palm J.,1(2), pp.275- 284,1982.
[14] Elleuch, M.; Besbes, S.; Roiseux, O.; Blecker, C.; Deroanne, C.; Drira, N. and Attia, H. Date flesh: chemical composition and characteristics of the dietary fibre. Food chemistry, 111, pp.676-682, 2008.
[15] Ahmed, I. A.; Ahmed, A. W. Chemical composition of date varieties as influenced by the stage of ripening. Food chemistry, 54, pp.305-309, 1995.
[16] Besbes, S., Blecker, C., and Deroanne, C. Date seeds: chemical composition and characteristic profiles of lipid fraction. Food Chemistry, 84, pp.577-584, 2004.
[17] Rahman, M.S., Kasapis, S., Al-Kharusi, N.S.Z., Al-Marhubi, I.M., and Khan, A.J.Composition characterisation and thermal transition of date pits powders. Journal of Food Engineering, 80, pp.1-10, 2007.
[18] Besbes, S.; Drira, L.; Blecker, C.; Deroanne, C. and Attia, H. Adding value to hard date (Phoenix dactylifera L.): compositional, functional and sensory characteristics of date jam. Food chemistry, 112, pp. 406- 411, 2009.
[19] Hulm, A.C. the biochemistry of fruit and their products. Academic press,London&New York, Vol. 2, 1971.