Determination of Penicillins Residues in Livestock and Marine Products by LC/MS/MS

Authors: Ji Young Song, Soo Jung Hu, Hyunjin Joo, Joung Boon Hwang, Mi Ok Kim, Shin Jung Kang, Dae Hyun Cho

Abstract:

Multi-residue analysis method for penicillins was developed and validated in bovine muscle, chicken, milk, and flatfish. Detection was based on liquid chromatography tandem mass spectrometry (LC/MS/MS). The developed method was validated for specificity, precision, recovery, and linearity. The analytes were extracted with 80% acetonitrile and clean-up by a single reversed-phase solid-phase extraction step. Six penicillins presented recoveries higher than 76% with the exception of Amoxicillin (59.7%). Relative standard deviations (RSDs) were not more than 10%. LOQs values ranged from 0.1 and to 4.5 ug/kg. The method was applied to 128 real samples. Benzylpenicillin was detected in 15 samples and Cloxacillin was detected in 7 samples. Oxacillin was detected in 2 samples. But the detected levels were under the MRL levels for penicillins in samples.

Keywords: Penicillins, livestock product, Multi-residue analysis, LC/MS/MS

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1058101

References:

[1] J.O. Boison, in; H. Oka, H. Nakazawa, K.I. Harada, J.D. Macneil (Eds.), Chemical Analysis for Antibiotics Used in Agriculture, AOAC International, Arlington, VA, 1995, pp. 235-306, Chapter 8.
[2] H. Oka, J. Patterson, in: H. Oka, H. Nakazawa, K.I. Harada, J.D. Macneil (Eds.), Chemical Analysis for Antibiotics Used in Agriculture, AOAC International, Arlington, VA, 1995, pp. 333-406, Chapter 10.
[3] T. Goto, Y. Ito, S. Yamada, H. Matsumoto, H. Oka, Journal of Chromatography A, 1100 (2005) 193-199.
[4] M. Becker, E. Zittlau, M. Petz, Anal. Chim. Acta 520(2004) 192.
[5] C,K. Fagerquist, A.R. Lightfield, Rapid Commun. Mass Spectrum. 17 (2003) 660.
[6] S.H. Hsieh, A.Y. Huang, S. Lee, Journal of Chromatography A, 1216 (2009)7186-7194.
[7] K. Bush, G.A. Jacoby, A.A. Medeiros, Antimicrob. Agents Chemother. 39 (1995) 1211.
[8] J.P. Hou, J.W. Poole, J. Pharm. Sci. 60 (1971) 503.
[9] P. Anfossi, A. Zaghini, G. Granssigli, S. Menotta, G. Fendrizzi, J. Vet, Pharmacol. Ther.25 92002) 329.
[10] B.A. Chowdhury, P.L. Lieberman, in; R.F. Lockey, S.C. Bukanz (Eds.), Allergens and Allergen Immunotheraphy, 2nd ed., Dekker, New York, 1999, p.273.
[11] C.K. Fagerquist, A.R. Lightfield, S.J. Lehotay, Anal. Chem. 77 (2005) 1473.
[12] C.Y.W. Ang, W. Luo, E.B. Hansen, J.P. Freeman, H.C. Thompson, J. AOAC Int. 79 (1996) 389.