Interspecific Variation in Heat Stress Tolerance and Oxidative Damage among 15 C3 Species

Authors: Wagdi S. Soliman, Shu-ichi Sugiyama

Abstract:

The C3 plants are frequently suffering from exposure to high temperature stress which limits the growth and yield of these plants. This study seeks to clarify the physiological mechanisms of heat tolerance in relation to oxidative stress in C3 species. Fifteen C3 species were exposed to prolonged moderately high temperature stress 36/30°C for 40 days in a growth chamber. Chlorophyll fluorescence (Fv/Fm) showed great difference among species at 40 days of the stress. The species showed decreases in Fv/Fm and increases in malondialdehyde (MDA) content under stress condition as well as negative correlation between Fv/Fm and MDA (r = -0.61*) at 40 days of the stress. Hydrogen peroxide (H2O2) content before and after stress in addition to its response under stress showed great differences among species. The results suggest that the difference in heat tolerance among C3 species is closely associated with the ability to suppress oxidative damage but not with the content of reactive oxygen species (ROS) which is regulated by complex network.

Keywords: C3 species, Fv/Fm, heat stress, oxidative stress.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1058011

References:

[1] D. B. Lobell, and G. P. Asner, "Climate and management contributions to recent trends in U.S. agricultural yields," Science, vol. 299, pp. 1032, 2003.
[2] D. B. Lobell, and C. B. Field, "Global scale climate-crop yield relationships and the impacts of recent warming," Environ. Res. Letters, vol. 2, 014002 (7 pp), 2007.
[3] B. Barnab├ís, K. J├ñger, and A. Fehér, "The effect of drought and heat stress on reproductive processes in cereals," Plant Cell Environ., vol. 31, pp. 11-38, 2008.
[4] M. A. Semenov, and N. G. Halford, "Identifying target traits and molecular mechanisms for wheat breeding under a changing climate," J. Exp. Bot., vol. 60, pp. 2791-2804, 2009.
[5] M. Tester, and A. Bacic, "Abiotic stress tolerance in grasses. From model plants to crop plants," Plant Physiol., vol. 137, pp. 791-793, 2005.
[6] Y. Zhang, M. A. R. Mian, and J. H. Bouton, "Recent molecular and genomic studies on stress tolerance of forage and turf grasses," Crop Sci., vol. 46, pp. 497-511, 2006.
[7] A. Wahid, S. Gelani, M. Ashraf, and M. R. Foolad, "Heat tolerance in plants: an overview," Environ. Exp. Bot., vol. 61, pp. 199-223, 2007.
[8] T. D. Sharkey, "Effects of moderate heat stress on photosynthesis: importance of thylakoid reactions, rubisco deactivation, reactive oxygen species, and thermotolerance provided by isoprene," Plant Cell Environ., vol. 28, pp. 269-277, 2005.
[9] V. Velikova, and F. Loreto, "On the relationship between isoprene emission and thermotolerance in Phragmites australis leaves exposed to high temperatures and during the recovery from a heat stress," Plant Cell Environ., vol. 28, pp. 318-327, 2005.
[10] V. Locato, C. Gadaleta, L. De Gara, and M. C. De Pinto, "Production of reactive species and modulation of antioxidant network in response to heat shock: a critical balance for cell fate," Plant Cell Environ., vol. 31, pp. 1606-1619, 2008.
[11] W. S. Soliman, M. Fujimori, K. Tase, and S. Sugiyama, "Oxidative stress and physiological damage under prolonged heat stress in C3 grass Lolium perenne," Grassland Sci., vol. 57, pp. 101-106, 2011.
[12] R. Mittler, S. Vanderauwera, M. Gollery, and F. Van Breusegem, "Reactive oxygen gene network of plants," Trends Plant Sci., vol. 9, pp. 490-498, 2004.
[13] G. Miller, N. Suzuki, S. Ciftci-Yilmaz, and R. Mittler, "Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant Cell Environ., vol. 33, pp. 453-467, 2010.
[14] L. Rizhsky, E. Hallak-Herr, F. Van Breusegem, S. Rachmilevitch, J. E. Barr, S. Rodermel, D. Inzé, and R. Mittler, "Double antisense plants lacking ascorbate peroxidase and catalase are less sensitive to oxidative stress than single antisense plants lacking ascorbate peroxidase or catalase," Plant J., vol. 32, pp. 329-342, 2002.
[15] G. Miller, N. Suzuki, L. Rizhsky, A. Hegie, S. Koussevitzky, and R. Mittler, "Double mutants deficient in cytosolic and thylakoid ascorbate peroxidase reveal a complex mode of interaction between reactive oxygen species, plant development, and response to abiotic stresses," Plant Physiol., vol. 144, pp. 1777-1785, 2007.
[16] B. Huang, X. Liu, and Q. Xu, "Supraoptimal soil temperatures induced oxidative stress in leaves of creeping bentgrass cultivars differing in heat tolerance. Crop Sci., vol. 41, pp. 430-435, 2001.
[17] J. Larkindale, and B. Huang, "Changes of lipid and saturation level in leaves and roots for heat-stressed and heat-acclimated creeping bentgrass (Agrostis stolonifera)," Environ. Exp. Bot., vol. 51, 57-67, 2004.
[18] M. Almeselmani, P. S. Deshmukh, R. K. Sairam, S. R. Kushwaha, and T. P. Singh, "Protective role of antioxidant enzymes under high temperature stress," Plant Sci., vol. 171, pp. 382-388, 2006.
[19] Y. He, and B. Huang, "Protein changes during heat stress in three kentucky bluegrass cultivars differing in heat tolerance," Crop Sci., vol. 47, pp. 2513-2520, 2007.
[20] S. Xu, J. Li, X. Zhang, H. Wei, and L. Cui, "Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultrastructure of chloroplast in two cool-season turfgrass species under heat stress," Environ. Exp. Bot., vol. 56, pp. 274- 285, 2006.
[21] Y. Xu, and B. Huang, "Differential protein expression for geothermal Agrostis scabra and turf-type Agrostis stolonifera differing in heat tolerance," Environ. Exp. Bot., vol. 64, pp. 58-64, 2008.
[22] C. Xu, and B. Huang, "Differential proteomic response to heat stress in thermal Agrostis scabra and heat-sensitive Agrostis stolonifera," Physiol. Plantarum, vol. 139, pp. 192-204, 2010.
[23] W. S. Soliman, M. Fujimori, K. Tase, and S. Sugiyama, "Heat tolerance and suppression of oxidative stress in: comparative analysis of 25 cultivars of the C3 grass Lolium perenne," Environ. Exp. Bot., vol. 78, pp. 10-17, 2012.
[24] J. M. Cheeseman, "Hydrogen peroxidase concentrations in leaves under natural conditions," J. Exp. Bot., vol. 57, pp. 2435-2444, 2006.
[25] G. Queval, J. Hager, B. Gakière, and G. Noctor, "Why are literature data for H2O2 contents so variable? A discuusion of potential difficulties in the quantitative assay of leaf extracts," J. Exp. Bot., vol. 59, pp. 135- 146, 2008.
[26] K. Maxwell, and G. N. Johnson, "Chlorophyll fluorescence- a practical guide," J. Exp. Bot., vol. 51, pp. 659-668, 2000.
[27] S. P. Long, S. Humphries, and P. G. Falkowski, "Photoinhibition of photosynthesis in nature," Ann. Rev. Plant Physiol. Plant Mol. Biol., vol. 45, pp. 633-662, 1994.
[28] C. H. Foyer, and G. Noctor, "Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context," Plant Cell Environ., vol. 28, pp. 1056-1071, 2005.
[29] N. Suzuki, and R. Mittler, "Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction. Physiol. Plantarum, vol. 126, pp. 45-51, 2006.
[30] P. Jaspers, and J. Kangasjärvi, "Reactive oxygen species in abiotic stress signaling," Physiol. Plantarum, vol. 138, pp. 405-413, 2010.
[31] K. Asada, "The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons," Ann. Rev. Plant Physiol. Plant Mol. Biol., vol. 50, pp. 601-639, 1999