A Study of the Cyclic Variations of the Enzyme and the Electrolyte Activity in Uterine and Oviducal Secretions during an Estrous Cycle of the Ewe

Authors: Yahia M., Laanani I., Benbia S., Hachemi M., Massinissa Y.

Abstract:

Uterine and oviducal fluids are necessary for capacitation of the spermatozoa and early embryonic development. The aim of the present study was to determine the effects of estrous cycle phases (follicular and luteal) on some biological parameters (enzymes, electrolytes and total proteins) in uterine and oviducal secretions of ewes. Oviducal and uterine fluids were collected, diluted and centrifuged. According to our results, concentrations of GPT, G6PDH, total proteins, K and Na were significantly (P <0.05) higher at the luteal phase, however, the levels of aldolase, Mg, Ca and P were significantly (P <0.05) higher at the follicular phase in uterine secretions. While, only oviducal K and Ca were significantly (P<0.05) higher at the follicular phase. Our study revealed the existence of significant cyclic variations for some uterine and oviducal parameters which indicates the effect of ovarian hormones on the components of genital secretions.

Keywords: Biochemical parameters, estrous cycle, ewe, genital secretion.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1327973

References:

[1] S. Hugentobler, D. Morris, J. Sreenan and M. Diskin, "Ion concentrations in oviduct and uterine fluid and blood serum during the estrous cycle in the bovine," Theriogenology, vol. 68, pp. 538-548, 2007.
[2] P. Lamothe , P. Guay, M. Ibrahim and A. Tremblay A,"Total proteins and their fractions in the serum and endometrial secretions in the cow with of sine materia infertility, "Can. J. comp. Med, vol. 36, pp.94-98, August. 1972.
[3] C. Thibault and M-C. Levasseur, " Reproduction in mammals and human," Nouv. Éd. ent. ref. ed. Paris: INRA : Ellipses, 2001:928 p., 928 p. de pl.2001.
[4] P. Guay and P. Lamothe, "Biochemical study of genital secretions of infertile bovine females with normal estrous cycles," Can. J. comp. Med, Vol. 33, pp.33-93-97, 1969.
[5] G.P. Roberts, J.M. Parker and H.W. Symonds,"Macromolecular components of genital tract fluids from the sheep,"J.Reprod.Fert, vol.107, pp.48-99, 1976.
[6] J.L. Perkins, L. Goode, W.A. Wilder and D.B. Henson,"Collection of Secretions from the Oviduct and Uterus of the Ewe," J Anim Sci, vol. 24, pp.383-387, 1965.
[7] M. L. Fahning, R. H. SChultz and E. F. Graham, "The free amino acid content of uterine fluids and blood serum in the cow," J. Reprod. Fert, vol.13, pp. 229-236, 1967.
[8] S.M. Alavi-Shoushtari, S. Asri-Rezai and J. Abshenas, "A study of the uterine protein variations during the estrous cycle in the cow: Molecular weights determination.Anim. Reprod," Science, vol. 105, pp. 302-310, 2006.
[9] D. Olds and N L. Vandemark, "Composition of luminal fluids of bovine female genitalia," Fertility Sterility, vol. 8, pp.345-354, 1957.
[10] M. L. Fahning, R. H. SChultz and E. F. Graham, "The free amino acid content of uterine fluids and blood serum in the cow," J. Reprod. Fert, vol.13, pp. 229-236, 1967.
[11] S.M. Alavi-Shoushtari, S. Asri-Rezai and J. Abshenas, "A study of the uterine protein variations during the estrous cycle in the cow: Molecular weights determination.Anim. Reprod," Science, vol. 105, pp. 302-310, 2006.