Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30184
Osteogenesis by Dextran Coating on and among Fibers of a Polyvinyl Formal Sponge

Authors: M. Yoshikawa, N. Tsuji, T. Yabuuchi, Y Shimomura, H. Kakigi, H. Hayashi, H. Ohgushi

Abstract:

A scaffold is necessary for tooth regeneration because of its three-dimensional geometry. For restoration of defect, it is necessary for the scaffold to be prepared in the shape of the defect. Sponges made from polyvinyl alcohol with formalin cross-linking (PVF sponge) have been used for scaffolds for bone formation in vivo. To induce osteogenesis within the sponge, methods of growing rat bone marrow cells (rBMCs) among the fiber structures in the sponge might be considered. Storage of rBMCs among the fibers in the sponge coated with dextran (10 kDa) was tried. After seeding of rBMCs to PVF sponge immersed in dextran solution at 2 g/dl concentration, osteogenesis was recognized in subcutaneously implanted PVF sponge as a scaffold in vivo. The level of osteocalcin was 25.28±5.71 ng/scaffold and that of Ca was 129.20±19.69 µg/scaffold. These values were significantly higher than those in sponges without dextran coating (p<0.01). Osteogenesis was induced in many spaces in the inner structure of the sponge with dextran coated fibers.

Keywords: Dextran, Polyvinyl formal sponge, Osteogenesis, Scaffold.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1056571

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1194

References:


[1] M. Satoyoshi, T. Koizumi, T. Teranaka, T. Iwamoto, H. Takita, Y. Kuboki, S. Saito, and Y. Mikuni-Takagaki, "Extracellular processing of dentin matrix protein in the mineralizing odontoblast culture," Calcif. Tissue Int., vol. 57, no. 3, 237-241, Sep. 1995.
[2] P. Papagerakis, A. Berdal, M. Mesbah, M. Peuchmaur, L. Malaval, J. Nydegger, J. Simmer, and M. Macdougall, "Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth," Bone, vol. 30, no. 2, pp. 377-385, Feb. 2002.
[3] W. K. Jeong, S. H. Oh, J. H. Lee, and G. I. Im, "Repair of osteochondral defects with a construct of mesenchymal stem cells and a polydioxanone/poly (vinyl alcohol) scaffold," Biotechnol. Appl. Biochem., vol. 49, no. 2, pp. 155-164, Feb. 2008.
[4] C. R. Nuttelman, D. J. Mortisen, S. M. Henry, and K. S. Anseth, "Attachment of fibronectin to poly(vinyl alcohol) hydrogels promotes NIH3T3 cell adhesion, proliferation, and migration," J. Biomed. Mater. Res., vol. 57, no. 2, pp. 217-223, Nov. 2001.
[5] J. A. Cadée, M. J. van Luyn, L. A. Brouwer, J. A. Plantinga, P. B. van Wachem, C. J. de Groot, W. den Otter, and W. E. Hennink, "In vivo biocompatibility of dextran-based hydrogels," J. Biomed. Mater. Res., vol. 50, no. 3, pp. 397-404, Jun. 2000.
[6] J. M. Liu, F. Haroun-Bouhedja, and C. Boisson-Vidal, "Analysis of the in vitro inhibition of mammary adenocarcinoma cell adhesion by sulfated polysaccharides," Anticancer Res., vol. 20, no. 5A, pp. 3265-3271, Sep-Oct. 2000.
[7] C. Maniatopoulos, J. Sodek, and A. H. Melcher, "Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats," Cell Tissue Res., vol. 254, no. 2, 317-330, Nov. 1988.
[8] H. Ohgushi, Y. Dohi, T. Katuda, S. Tamai, S. Tabata, and Y. Suwa, "In vitro bone formation by rat marrow cell culture," J. Biomed. Mater. Res., vol. 32, no. 3, pp. 333-340, Nov. 1996.
[9] D. Lemus, "Contributions of heterospecific tissue recombinations to odontogenesis," Int. J. Dev. Biol., vol. 39, no. 1, pp. 291-297, Feb. 1995.
[10] J. Lewin-Epstein, B. Azaz, and M. Ulmansky, "Fate of osteogenic tissue transferred to the subcutaneous area by means of polyvinyl-formal sponge," Isr. J. Med. Sci., vol. 5, no. 3, pp. 365-372, May-Jun. 1969.
[11] E. Tsuruga, H. Takita, H. Itoh, Y. Wakisaka, and Y. Kuboki, "Pore size of porous hydroxyapatite as the cell-substratum controls BMP-induced osteogenesis," J. Biochem., vol. 121, no. 2, pp. 317-324, Feb. 1997.
[12] M. B. Zajaczkowski, E. Cukierman, C. G. Galbraith, and K. M. Yamada, "Cell-matrix adhesions on poly (vinyl alcohol) hydrogels," Tissue Eng., vol. 9, no. 3, pp. 525-533, Jun. 2003.
[13] Y. W. Wang, Q. Wu, and G. Q. Chen, "Reduced mouse fibroblast cell growth by increased hydrophilicity of microbial polyhydroxyalkanoates via hyaluronan coating," Biomaterials, vol. 24, no. 25, pp. 4621-4629, Nov. 2003.
[14] D. Li, K. Dai, and T. Tang, "Effects of dextran on proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells," Cytotherapy, vol. 10, no.6, pp. 587-596, Jun. 2008.
[15] M. G. Cascone, S. Maltinti, N. Barbani, and M. Laus, "Effect of chitosan and dextran on the properties of poly (vinyl alcohol) hydrogels," J. Mater. Sci. Mater. Med.., vol. 10, no.7, pp. 431-435, Jul. 1999.