Influence of Degradative Enzymatic Activities on the Shelf Life of Ready-to-Eat Prickly Pear Fruits

Authors: D. Scalone, R. Palmeri, F. Licciardello, G. Muratore, A. Todaro, G. Spagna

Abstract:

Prickly pear fruit (Opuntia ficus indica L. Miller) belongs to the Cactaceae family. This species is very sensitive to low storage temperatures (< 5°C) which cause damages. The fruits can be peeled, suitably packaged and successfully commercialized as a ready-to-eat product. The main limit to the extension of the shelf life is the production of off-flavors due to different factors, the growth of microorganisms and the action of endogenous enzymes. Lipoxygenase (LOX) and Pectinesterase (PE) are involved in fruit degradation. In particular, LOX pathway is directly responsible for lipid oxidation, and the subsequent production of off-flavours, while PE causes the softening of fruit during maturation. They act on the texture and shelf-life of post-harvest, packaged fruits, as a function of the the grown of microorganisms and packaging technologies used. The aim of this work is to compare the effect of different packaging technologies on the shelf life extension of ready-to-eat prickly pear fruits with regards for the enzymes activities.

Keywords: Enzymes, packaging, prickly pear, shelf life.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1056040

References:

[1] L.F. Di Cesare, A. Testoni, G. Sansovini, "Studio dei componenti volatili del fico d'india" in Industrie alimentary, vol 32 pp. 725-733. 1993.
[2] M. Chisari, R.N. Barbagallo, G. Spagna, F. Artes, "Improving the quality of fresh-cut melon through inactivation of degradative oxidase and pectinase enzymatic activities by UV-C treatment", Int J Food Sci Tech, vol 46, pp. 463-468, Feb. 2011.
[3] R.N. Barbagallo, M. Chisari, F. Branca, G. Spagna, "Pectin Methylesterase, polyphenol oxidase and physicochemical properties of typical long-storage cherry tomatoes cultivated under water stress regime", J Sci Food Agr, vol 88, pp. 389-396, Feb. 2008.
[4] G.A. Tucker , "Introduction", in Biochemistry of Fruit Ripening, In G.B. Seymour, J.E. Taylor, and G.A: Tucker, Ed Chapman & Hall, London, 1993, pp. 1-51.
[5] U. de 0. Bicalho, M. de V. Camargo Penteado, "Estudio de fruto e do articuloda Opuntia ficus indica (L.) Miller cultivada em Valinhos, S. P. II. Caracteristica bioquimicas", Rev. Farm. Bioquim. Univ. S. Paulo vol 18, pp.68-'74, 1982.
[6] Y. Luo, "Effects of lipoxygenase on the postharvest physiology of tomato fruit" Acta Hort. Sinica vol 21, pp. 357-360, 1994.
[7] Y. Rogiers-Suzy, G.N.M. Kumar, and N.R. Knowles, "Maturation and ripening of Amelanchier Nutt. Are accompanied by increasing oxidative stress", Ann. Bot. London vol 81, pp. 203-211, 1988.
[8] B. Zhang, X.-R. Yin, J.-Y. Shen, K.-S. Chen, “Volatiles production and lipoxygenase gene expression in kiwifruit peel and flesh during fruit ripening”, J. Amer. Soc. Hort. Sci., vol. 134, no 4, pp. 472-477, 2009.
[9] P.M. Ealing, “Lipoxygenase activity in ripening tomato fruit pericarp tissue”, Phytochemistry, vol 36, pp. 547-552.
[10] M. Cantwell, “Fresh cut products” Perish. Handli. Newsl., vol 81, pp. 2- 3, 1995.
[11] H. Izumi, A.E. Watada, W. Douglas, “Optimum O2 or CO2 Atmosphere for storing Broccoli Florets at Various Temperatures”, J. Amer. Soc. Hort. Sci., vol 121, no 1, pp. 127-131, 1996.
[12] A.E. Hagerman P.J. Austin, “Continuous spectrophotometric assay for plant pectin methylesterase, J. Agric. Food Chem., vol 34, pp. 440-444.
[13] G. Spagna, R.N. Barbagallo, B. Ingallinera, “A specific method for determination of pectin esterase in blood oranges”, Enzyme Microb. Technol., vol 32, pp. 174-177.
[14] M. Ridolfi, S. Terenziani, M. Patumi, G. Fontanazza, “Characterization of Lipoxygenase in some olive cultivars and determination of their role in volatile compounds formation”, J. Agric. Food Chem., vol 50, pp. 835-839, 2002.
[15] E. A Gordon, D.M. Barret, “Colorimetric Method for the determination of lipoxygenase activity”, J.Agric. Food Chem., vol 49, pp. 32-37, 2001.
[16] R. Barbagallo, G. Spagna, A. Renda, G. Ruberto, “Determinazione di alcuni componenti chimici dei succhi e dell’olio di semi di fico d’india (opuntia ficus indica (L.) Mill)”, Ricerca e Innovazioni nell’industria Alimentare, Chirotti, Ed., Pinerolo, Italy 4, 1139.
[17] M. Cantwell, “Postharvest management of fruits and vegetable stems”, in Agro-ecology, cultivation and uses of cactus pear, G. Barbera, P. Inglese and P. Pimienta-Barrios, Ed. FAO Plant Production and Protection Paper 132, 1995, pp. 120-136.