In vitro Plant Regeneration of Java Vetiver (Vetiveria zizanioides)

Authors: Iriawati, R. R. Esyanti, W. Natalia, N. Zahya

Abstract:

In vitro plant regeneration has been successfully obtained from basal shoot explant of Vetiveria zizanioides through indirect organogenesis. The explant was cultured in Murashige & Skoog’s (MS) media supplemented with 2,4-D, IAA, and kinetin in various concentrations. Callus was well induced in media supplemented with 2 ppm 2,4-D, 1 ppm IAA, and 1 ppm kinetin. This callus was then transferred to MS media supplemented with 1 - 5 ppm of BAP for shoot regeneration. The media supplemented with 3 ppm BAP was a suitable medium for shoot induction, as well as for shoot multiplication. Rooting was well developed in shoot following transferred to half MS media containing 0.2 ppm IBA. Plantlet was then transferred to husk charcoal for acclimatization, and almost all (90%) of plantlets were survived during acclimatization.

Keywords: Callus, plantlet regeneration, shoot induction, Vetiveria zizanioides.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1087684

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