Solid-State Bioconversion of Pineapple Residues into Kojic Acid by Aspergillus flavus: A Prospective Study
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 33122
Solid-State Bioconversion of Pineapple Residues into Kojic Acid by Aspergillus flavus: A Prospective Study

Authors: S. Nurashikin, E. Z. Rusley, A. Husaini

Abstract:

Kojic acid is an organic acid that is widely used as an ingredient for dermatological products, precursor for flavor enhancer and also as anti-inflammatory drug. The present study was undertaken to test the feasibility of pineapple residues as substrate for kojic acid production by Aspergillus flavus Link 44-1 via solid-state fermentation. The effect of initial moisture content, pH and incubation time on kojic acid fermentation was investigated. The best initial moisture content for kojic acid production from pineapple residues was observed at 70% (v/w) whereas initial culture pH 2.5 was identified to give high production of kojic acid. The optimal range of incubation time was identified between 8 and 14 days of incubation which corresponded to highest range of kojic acid produced. The results from this study pronounce the promising usability of pineapple residues as alternative substrate for kojic acid production by A. flavus Link 44-1.

Keywords: Aspergillus flavus, kojic acid, pineapple residues, solid state fermentation.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1087636

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2692

References:


[1] S. Ketnawa, P. Chaiwut, and S. Rawdkuen, “Pineapple wastes: A potential source for bromelain extraction,” Food and Bioproducts Processing, 90, pp. 385–391, 2012.
[2] K. A. Buckle, “Biotechnology Opportunities in Waste Treatment and Utilisation for the Food Industry,” In Biotechnology and The Food Industry, Ed. Rogers P.L. Gordon and Breach Science Publishers, New York, pp. 261-277, 1989.
[3] Abdullah, “Solid and liquid pineapple waste utilization for lactic acid fermentation using Lactobacillus delbrueckii,” Reaktor, vol. 11, no 1, pp. 50-52, 2007.
[4] K. Tanaka, Z. D. Hilary, and A. Ishizaki, ” Investigation of the utility of pineapple juice and pineapple waste material as low-cost substrate for ethanol fermentation by Zymomonas mobile,” Journal of Bioscience and Bioengineering. vol. 87, pp. 642–646, 1999.
[5] D. Kumar, V. K. Jain, G. Shanker, and A. Srivastava, “Utilisation of fruits waste for citric acid production by solid state fermentation,” Process Biochemistry, vol. 38, pp. 1725–1729, 2003.
[6] T. Chang, “An updated review of tyrosinase inhibitors,” International Journal of Molecular Sciences, vol. 10, pp. 2440-2475, 2009.
[7] A. J. Gomes, C.N. Lunardi, S. Gonzalez, and A. C. Tedesco, “The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species,” Brazillian Journal of Medical and Biological Research, vol. 34, pp. 1487-1494, 2001.
[8] G.A. Burdock, M. G. Soni, and I. G. Carabin, “Evaluation of health aspects of kojic acid in food,” Regulatory Toxicology and Pharmacology, vol. 33, pp. 214-228, 2001.
[9] M. Rosfarizan, “ Kinetics, modelling and scaling-up of kojic acid fermentation by Aspergillus flavus 44-1 using different carbon sources,” PhD thesis. Universiti Pertanian Malaysia. 2000.
[10] G. L. Miller, “ Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Analytical Chemistry, vol. 31, no. 3, pp. 426-428, 1959.
[11] R. Bently, “Preparation and analysis of kojic acid.” Enzymo, vol. 3, pp. 238-241, 1957.
[12] M. Rosfarizan, A. B. Ariff, M.A. Hassan, and M. I. A. Karim, “Influence of pH on kojic acid fermentation by Aspergillus flavus.” Pakistan Journal of Biological Sciences, vol. 6, pp. 977-982, 2000.
[13] M. Kitada, H. Ueyama, and T. Fukimbara, “Studies on kojic acid fermentation (I) Cultural condition in submerged culture,” Journal of Fermentation Technology, vol. 4, pp. 1101-1107, 1967.