Protective Effect of Ethanolic Extract of Polyherbal Formulation on Carbon Tetrachloride Induced Liver Injury

Authors: R. Kokilavani, K. Gurusamy, K. Arumugasamy

Abstract:

Protective effect of ethanolic extract of polyherbal formulation (PHF) was studied on carbon tetrachloride induced liver damage on carbon tetrachloride induced liver damage. Treatment of rats with 250mg /kg body weight of ethanolic extract of PHF protected rats against carbon tetrachloride liver injury by significant lowerering 5’ nucleotidase (5’NT), Gamma Glutamyl transferase (GGT), Glutamate dehdyrogenasse (GDH) and Succinate Dehydrogenase (SDH) levels compared to control. Normalization in these enzyme levels indicates strong hepatoprotective property of PHF extract.

Keywords: Carbon tetrachloride, ethanolic, hepatoprotective, polyherbal formulation.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1086979

References:

[1] Shivkumar SI, Suresh HM, Vrushabendra swamy BM, Kumar GS, Dhanapal R, Ashokbabu VL, Rao V. (2006) Hepatoprotective activity of fruits of Cocinia grandis Linn. against carbon tetrachloride induced hepatotoxicity. J. Adv. Pharmacol. and Toxicol. 7(2): 7-9.
[2] Sampathkumar, R., Sivakumar, T., Sivakumar, P. and Nethaji, R. (2005). Hepatoprotective and in vivo antioxidant effects of Careya arborea against carbon tetrachloride-induced liver damage in rats. International Journal of Molecular Medicine and Advance Sciences, 1 (4): 418-424.
[3] Defeudis, F.V., Papdopoulos, V. and Drieu, K. (2003). Ginko biloba extracts and cancer a research area in its infancy. Fundamentals of Clinical Pharmacology, 17: 405-417.
[4] Campbell, D.M. (1962). Determination of 5’nucleotidase in blood serum, Biochemical Journal, 82: 34.
[5] Persijn, J. P. and Vanderslik, W. (1976). A new method for the determination of gamma glutamyl transferase in serum. Journal of Clinical Chemistry and Clinical Biochemistry, 14: 421- 427.
[6] Sadasivam, S. and Manickam, A. (2005). Estimation of glutamate dehydrogenase, Biochemical methods, New age International publishers, revised second edition, 99-101.
[7] Slater, E. C. and Bonner, W.D. (1952). Succinate dehydrogenase. Biochemical Journal, 52: 182-196.
[8] Daniel WW. (2006) Biostatistics, A foundation for analysis in the health Sciences. Seventh edition, pub. Wiley India. 295-399.
[9] Pradeep, K., Mohan, C.V., Anand, K.G. and Karthikeyan, S. (2005). Effect of pretreatment of Cassia fistula Linn. leaf extract against subacute CCl4 induced hepatotoxicity in rats. Indian Journal of Experimental Biology, 43 (6): 526-530.
[10] Gupta, S., Pandey, R., Katyai, R., Aggarwal, H.K., Aggarwal, R.P. and Aggarwal, S.K. (2005). Lipid peroxide levels and antioxidant status in alcoholic liver disease. Indian Journal of Clinical Biochemistry, 20 (1): 67-71.
[11] Venukumar, M.R. and Latha, M.S. (2004). Effect of Coscinium fenestratum on hepatotoxicity in rats. Indian Journal of Experimental Biology, 42: 792-797.
[12] Bishayee, A., Sarkar, A. and Chatterjee, M. (1995). Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver. Journal of Ethnopharmacology, 47: 69-74.
[13] Shukla, S., Jadon, A. and Bhadauria, M. (2005). Comparative effect of Terminalia belerica fruit extract and its active principle against carbon tetrachloride-induced toxicity in rats. Indian Journal of Pharmaceutical Science, 67 (6): 681-686.
[14] Shukla, S., Jadon, A., Bhadauria, M. and Sharma, A. (2006). Ameliorative effects of proprietary herbal formulation on carbon tetrachloride induced biochemical and histopathological changes in rats. Indian Drug, 43 (3): 235-244.