Secondary Ion Mass Spectrometry of Proteins

Authors: Santanu Ray, Alexander G. Shard

Abstract:

The adsorption of bovine serum albumin (BSA), immunoglobulin G (IgG) and fibrinogen (Fgn) on fluorinated selfassembled monolayers have been studied using time of flight secondary ion mass spectrometry (ToF-SIMS) and Spectroscopic Ellipsometry (SE). The objective of the work has to establish the utility of ToF-SIMS for the determination of the amount of protein adsorbed on the surface. Quantification of surface adsorbed proteins was carried out using SE and a good correlation between ToF-SIMS results and SE was achieved. The surface distribution of proteins were also analysed using Atomic Force Microscopy (AFM). We show that the surface distribution of proteins strongly affect the ToFSIMS results.

Keywords: ToF-SIMS, Spectroscopic Ellipsometry, Protein, Atomic Force Microscopy.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1086247

References:

[1] Ramsden, J.J., Experimental methods for investigating protein adsorption kinetics at surfaces. Quarterly Reviews of Biophysics, 1993. 27(01): p. 41-105.
[2] Michel, R. and D.G. Castner, Advances in time-of-flight secondary ion mass spectrometry analysis of protein films. Surface and Interface Analysis, 2006. 38(11): p. 1386-1392.
[3] Wagner, M.S. and D.G. Castner, Analysis of adsorbed proteins by static time-of-flight secondary ion mass spectrometry. Applied Surface Science, 2004. 231-232: p. 366-376.
[4] Wagner, M.S. and D.G. Castner, Characterization of Adsorbed Protein Films by Time-of-Flight Secondary Ion Mass Spectrometry with Principal Component Analysis. Langmuir, 2001. 17(15): p. 4649 - 4660.
[5] Wagner, M.S., et al., Quantitative analysis of binary adsorbed protein films by time of flight secondary ion mass spectrometry. Journal of Biomedical Materials Research Part A, 2003. 64A(1): p. 1-11.
[6] Vanden Eynde, X. and P. Bertrand, Quantification of polystyrene blend surfaces based on end group ToF-SIMS analysis. Applied Surface Science, 1999. 141(1-2): p. 1-20.
[7] Kim, Y.-P., et al., Quantitative ToF-SIMS study of surfaceimmobilized streptavidin. Applied Surface Science, 2006. 252(19): p. 6801-6804.
[8] Kim, Y.-P., et al., Protein quantification on dendrimer-activated surfaces by using time-of-flight secondary ion mass spectrometry and principal component regression. Applied Surface Science, 2008. 255(4): p. 1110-1112.
[9] Wagner, M.S., T.A. Horbett, and D.G. Castner, Characterization of the Structure of Binary and Ternary Adsorbed Protein Films Using Electron Spectroscopy for Chemical Analysis, Time-of-Flight Secondary Ion Mass Spectrometry, and Radiolabeling. Langmuir, 2003. 19(5): p. 1708-1715.
[10] Ray, S. and A.G. Shard, Quantification of surface adsorbed proteins: A comparison of techniques. 2011, National Physical Laboratory: Teddington, London, UK.
[11] Ray, S., et al., Influence of work of adhesion on the dynamic wetting of chemically heterogeneous surfaces. Langmuir, 2008. 24(22): p. 13007 - 13012.
[12] Tsargorodskaya, A., A.V. Nabok, and A.K. Ray, Ellipsometric study of the adsorption of bovine serum albumin into porous silicon. Nanotechnology, 2004. 15(5): p. 703-709.
[13] Schwieters, J., et al., High mass resolution surface imaging with a time-of-flight secondary ion mass spectroscopy scanning microprobe. Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films, 1991. 9(6): p. 2864-2871.
[14] Horcas, I., et al., WSXM: A software for scanning probe microscopy and a tool for nanotechnology. Review of Scientific Instruments, 2007. 78(1).