Promotion of Growth and Modulation of As- Induced Stress Ethylene in Maize by As- Tolerant ACC Deaminase Producing Bacteria

Authors: Charlotte C. Shagol, Tongmin Sa

Abstract:

One of the major pollutants in the environment is arsenic (As). Due to the toxic effects of As to all organisms, its remediation is necessary. Conventional technologies used in the remediation of As contaminated soils are expensive and may even compromise the structure of the soil. An attractive alternative is phytoremediation, which is the use of plants which can take up the contaminant in their tissues. Plant growth promoting bacteria (PGPB) has been known to enhance growth of plants through several mechanisms such as phytohormone production, phosphate solubilization, siderophore production and 1-aminocyclopropane-1- carboxylate (ACC) deaminase production, which is an essential trait that aids plants especially under stress conditions such as As stress. Twenty one bacteria were isolated from As-contaminated soils in the vicinity of the Janghang Smelter in Chungnam Province, South Korea. These exhibited high tolerance to either arsenite (As III) or arsenate (As V) or both. Most of these isolates possess several plant growth promoting traits which can be potentially exploited to increase phytoremediation efficiency. Among the identified isolates is Pseudomonas sp. JS1215, which produces ACC deaminase, indole acetic acid (IAA), and siderophore. It also has the ability to solubilize phosphate. Inoculation of JS1215 significantly enhanced root and shoot length and biomass accumulation of maize under normal conditions. In the presence of As, particularly in lower As level, inoculation of JS1215 slightly increased root length and biomass. Ethylene increased with increasing As concentration, but was reduced by JS1215 inoculation. JS1215 can be a potential bioinoculant for increasing phytoremediation efficiency.

Keywords: As-tolerant bacteria, plant growth promoting bacteria, As stress, phytoremediation.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1085760

References:

[1] Agency for Toxic Substances and Disease Registry (ATSDR). Detailed data for 2011 priority list of hazardous substances. 2011. http://www.atsdr.cdc.gov/SPL/resources/ATSDR_2011_DPL_Detailed_Data_Table.pdf.
[2] Nriagu, J.O., P. Bhattacharya, A.B. Mukherjee, J. Bundschuh, R. Zevenhoven and R.H. Loppert. Arsenic in soil and groundwater: an overview. In Arsenic in Soil and Groundwater Environment. P. Bhattacharya et al. (eds). Trace Metals and Other Contaminants in the Environment. 2007, Vol. 9, pp. 3-60.
[3] Pacyna J.M. and E.G. Pacyna. An assessment of global and regional emissions of trace metals in the atmosphere from anthropogenic sources world. Environ. Rev. 2001, 9:269-298.
[4] Meharg AA, Hartley-Whitaker J. Arsenic uptake and metabolism in arsenic resistant and non-resistant plant species. New Phytologist. 2002, 154: 29–43.
[5] Rajkumar, M, Prasad, M.N.V., Freitas, H., Ae, N. Biotechnological applications of serpentine soil bacteria for phytoremediation of trace metals. Crit Rev. Biotechnol. 2009, 29:120-130.
[6] Pulford, I.D. and C. Watson. Phytoremediation of heavy metal-contaminated land by trees- A review. Environ. Int. 2003, 29:529-540.
[7] Glick, B. R. Using soil bacteria to to facilitate phytoremediation, Biotech Adv. 2010 28: (3) 367-374.
[8] Zhuang, X., J. Chen, H. Shim, and Z. Bai, New advances in plant growth promoting rhizobacteria for bioremediation. Environ. Int. 2007, 33: 406-413.
[9] Jiang, C. Y., Sheng, X. F., Qian, M., Q. Y., Isolation and characterization of a heavy metal-resistant Burkholderia sp. from heavy metal-contaminated paddy field soil and its potential in promoting plant growth and hevy metal accumulation in emtal-polluted soil. J. Appl. Microbiol. 2011, 5:1065-1074.
[10] Sheng, X. F., Xia, J. J. Improvement of rape (Brassica napus) plant growth and cadmium uptake by cadmium-resistant bacteria. Chemosphere. 2006, 64: 1036-1042.
[11] Zaidi, S., Usmani, S., Singh, B. R., Musarrat, J. Significance of Bacillus subtilis strain SJ-101 as a bioinoculant for concurrent plant growht promotion and nickel accumulation in Brassica juncea. Chemosphere. 2006, 64: 991-997.
[12] Burd, G. I., Dixon, D. G., Glick, B. R., A plant growth-promoting baterium that decreases nickel toxicity in seedlings. Appl. Environ. Microbiol. 1998, 64: 3663-3668.
[13] Bano, N., J. Musarrat. Characterization of a new Pseudomonas aerunginosa strain NJ-15 as a potential biocontrol agent. Curr. Microbiol. 2003, 46: 324-328.
[14] Penrose, D.M., Glick, B.R. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol. Plant. 2003, 118:10-15.
[15] Honma, M., Shimomura, T. Metabolism of 1- aminocyclopropane-1- carboxylic acid. Agri. Biol. Chem. 1978, 42: 1825-1831.
[16] Mehta, S., Nautiyal, C.S. () An efficient method for qualitative screening of phosphate-solubilizing bacteria. Curr. Microbiol. 2001, 43: 51-56.
[17] Murphy, J. and J.P. Riley. 1962. A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta. 27: 31-36.
[18] Alexander, B., Zuberer, D.A. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol. Fertil. Soils. 1991, 12:39-45.
[19] Chen X, Shi J, Chen Y, Xu X, Xu S, Wang Y.2006.Tolerance and biosorption of copper and zinc by Pseudomonas putida CZ1 isolated from metal-polluted soil. Can J Microbiol.52 (4):308-16.
[20] Nies DH. 1999. Microbial heavy-metal resistance.Appl Microbiol Biotechnol. 51(6):730-50.
[21] Singh N., Ma L.Q., Srivastava M., Rathinasabapathi, B.2006. Metabolic adaptations to arsenic-induced oxidative stress in Pteris vittata L. and Pteris ensiformis L. Plant Sci 170:274-282
[22] Ullrich-Eberius C.I., Sanz A., Novacky A.J. 1989. Evaluation of arsenate- and vanadate-associated changes of electrical membrane potential and phosphate transport in Lemna gibba G1. Journal of Experimental Botany 40: 119-128.