Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31097
Human Elastin-derived Biomimetic Coating Surface to Support Cell Growth

Authors: Antonella Bandiera


A new sythetic gene coding for a Human Elastin-Like Polypeptide was constructed and expressed. The recombinant product was tested as coating agent to realize a surface suitable for cell growth. Coatings showed peculiar features and different human cell lines were seeded and cultured. All cell lines tested showed to adhere and proliferate on this substrate that has been shown also to exert a specific effect on cells, depending on cell type.

Keywords: Coating, elastin, cell adhesion, recombinant protein

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1510


[1] J.Y. Lim and H.J. Donahue "Cell sensing and response to micro- and nanostructured surfaces produced by chemical and topographic patterning," Tissue Eng., vol. 13, pp. 1879-1891, Aug. 2007.
[2] P. Roach, D. Eglin, K. Rohde and C.C. Perry "Modern biomaterials: a review - bulk properties and implications of surface modifications," J. Mater. Sci. Mater. Med., vol. 18, pp. 1263-1277, July 2007.
[3] M.S. Bretscher "On the shape of migrating cells-a 'front-to-back' model," J. Cell Sci., vol.121, pp. 2625-2628, Aug. 2008
[4] M.P. Lutolf and J.A. Hubbell "Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering," Nat. Biotechnol.,vol. 23, pp. 47-55, Jan. 2005.
[5] V.A. Schulte, M. D├¡ez, M. Möller and M.C. Lensen "Surface topography induces fibroblast adhesion on intrinsically nonadhesive poly(ethylene glycol) substrates," Biomacromolecules, vol. 10, pp. 2795-2801, Oct. 2009.
[6] K.S. Straley and S.C. Heilshorn "Design and adsorption of modular engineered proteins to prepare customized, neuron-compatible coatings," Front. Neuroengineerin., vol. 2., pp. -10, Jun. 2009.
[7] V. Hasirci and H. Kenar "Novel surface patterning approaches for tissue engineering and their effect on cell behavior," Nanomed., vol. 1, pp. 73- 90. Jun. 2006.
[8] Y. Ikada "Challenges in tissue engineering," J. R. Soc. Interface, vol. 3, pp. 589-601, Oct. 2006.
[9] W.L. Grayson, T.P. Martens, G.M. Eng, M. Radisic and G. Vunjak- Novakovic "Biomimetic approach to tissue engineering," Semin Cell Dev. Biol., vol. 20, 665-673, Aug. 2009.
[10] A. Chilkoti, T. Christensen and J.A. MacKay "Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology," Curr. Opin. Chem. Biol., vol. 10, pp. 652-657, Dec. 2006.
[11] D.W. Urry " Characterization of soluble peptides of elastin by physical techniques," Methods Enzymol., vol. 82, pt A, pp. 673-716, 1982.
[12] D.W. Urry, T. Hugel, M. Seitz, H.E. Gaub, L. Sheiba, J. Dea, J. Xu and T. Parker. "Elastin: a representative ideal protein elastomer," Philos. Trans. R. Soc. Lond. B Biol. Sci., vol. 357, pp.169-184, Feb. 2002.
[13] A. Chilkoti, M.R. Dreher and D.E. Meyer., "Design of thermally responsive, recombinant polypeptide carriers for targeted drug delivery," Adv. Drug Deliv. Rev., vol. 54, pp. 1093-1111, Oct. 2002.
[14] A. Nagarsekar, J. Crissman, M. Crissman, F. Ferrari, J. Cappello and H. Ghandehari "Genetic engineering of stimuli-sensitive silkelastin-like protein block copolymers," Biomacromolecules, vol. 4, pp. 602-607, May-Jun 2003.
[15] J.C. Rodríguez-Cabello, S. Prieto, J. Reguera, F.J. Arias and A. Ribeiro, "Biofunctional design of elastin-like polymers for advanced applications in nanobiotechnology," J. Biomater. Sci. Polym. Ed., vol. 18, pp. 269- 286, 2007.
[16] C.M. Bellingham, K.A. Woodhouse, P. Robson, S.J. Rothstein and FW Keeley, "Self-aggregation characteristics of recombinantly expressed human elastin polypeptides," Biochim. Biophys. Acta, vol. 1550, pp. 6- 19, Nov 2001.
[17] A. Bandiera, A. Taglienti, F. Micali, B. Pani, M. Tamaro, V. Crescenzi and G. Manzini "Expression and characterization of human-elastinrepeat- based temperature-responsive protein polymers for biotechnological purposes," Biotechnol. Appl. Biochem., vol. 42, pp. 247-256, Dec 2005.
[18] A. Bandiera "Assembly and optimization of expression of synthetic genes derived from the human elastin repeated motif," Prep. Biochem. Biotechnol., to be published.
[19] R. Langer and D.A. Tirrell "Designing materials for biology and medicine," Nature, vol. 428, pp. 487-492, Apr. 2004.
[20] D. Chow, M.L. Nunalee, D.W. Lim, A.J. Simnick and A. Chilkoti "Peptide-based Biopolymers in Biomedicine and Biotechnology," Mater. Sci. Eng. R. Rep., vol. 62, pp. 125-155, Jan 2008.