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Study the Biological Activities of Tribulus Terrestris Extracts

Authors: Ahmed A. Hussain, Abbas A. Mohammed, Heba. H. Ibrahim, Amir H. Abbas


In this study the extracts of the Iraqi herb Tribulus terrestris (Al-Hassage or Al-Kutub) was done by using of polar and non polar solvents, then the biological activity of these extractants was studied in three fields, First, the antibacterial activity (in vitro) on gram positive bacteria (Staphylococcus aureus), and gram negative bacteria (E. coli, Proteus vulgaris, Pseudomonas aerugiuosa, and Klebsiella), all extracts showed considerable activity against all bacteria. Second, the effect of extracts on free serum testosterone level in male mice (in vivo), the alcoholic, and acetonitrilic extracts showed significant (P < 0.05) increase in free serum testosterone level, and we found that the extracts contained compounds with less genotoxic effects in mice germ cells. 3rd, was to study the effect of methanolic extract of T. terrestris in diabetes management.

Keywords: genotoxic, testosterone, germ cells, tribulus terrestris

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[1] R. A. Mothana, and U. Lindequist. Antimicrobial activity of some medicinal plants of the island Soqotra. J. Ethnopharmacol., 2005, 96(1- 2):177-181.
[2] K, Abeywickrama, and G. A. Bean. Toxigenic Aspergillus flavus and aflatoxins in Sri Lankan medicinal plant material. Mycopa-thologia., 1991, 113: 187-190.
[3] K. Gauthaman, A. P. Ganesan, and R. N. Prasad. "Sexual effects of puncturevine (Tribulus terrestris) extract (protodioscin): an evaluation using a rat model". Journal of Alternative and Complementary Medicine., 2003, 9 (2): 257-265.
[4] S. H. Majeed, and M. J. Mahmood. Herbs and Medicinal Plants in Iraq between Traditional Medicine and Scientific Research. 1st Ed. Baghdad: Dar Al-Thaowra for Publishing., 1988, p. 40. (in Arabic).
[5] S. Aldein. Medicinal Herbs. 1st Ed. Baghdad: Dar Al-Shoun Al- Thaqafia Al-Aama for Publishing.1986, p. 70. (in Arabic).
[6] M. M. Cowan. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 1999, 12(4): 564-582.
[7] O. Kandil, N. M. Radwan, A. B. Hassan, A. M. Amer, and H. A. El- Banna. Extracts and fractions of Thymus capitatus exhibit antimicrobial activities. J Ethnopharmacol. 1994, 44(1):19-24.
[8] D. McCan, L. Kirkis. Evaluation of Free Testosterone in serum. J Clin Immunoassay. 1985, 8: 234-6.
[9] M. R. Atlas, E. Alfres, and C. Lawrence "Laboratory Manual Experimental Microbiology" Mosby- Year Book, Inc. 1995.
[10] E. Evans, G. Breckon, and C. Ford.Air drying method for meiotic preparation from mammalian testes. Cytogenetics 1964, 3: 284-294.
[11] E. Bedir, A. Khan. New steroidal glycosides from the fruits of Tribulus terrestris. Pharmazie 2000, 57:491-3.
[12] Y. Wan, K. Ohtani, R. Kasai, and K. Yamasaki, Steroidal saponins from fruits of Tribulus terrestris. Phytochemistry 1997, 45:811-7.
[13] A. G. Brown, M. D. Vukovich, E. R. Martini, L. M. Kohut, W. D. Frank, DA Jackson, et al . Endocrine and lipid responses to chronic androstenediol-herbal supplementation in 30 to 58 year old men. J Am Coll Nutr 2002, 20:520-8.
[14] K. M. Arsyad. Effect of protodioscin on the quality and quantity of sperms from males with moderate idiopathic oligozoospermia. Medica 1996, 22:614-8.
[15] S. K. Bhattacharya. National conference on Recent trends in Spice and Medicinal Plants Research. Calcutta. India: April 1998, p. 652-4.
[16] B. Sun, W. Qu, and Z. Bai. The inhibitory effect of saponins from Tribulus terrestris on Bcap-37 breast cancer line in vitro. Zhong Yao Cai 2003, 26:104-6.
[17] Castoldi, G.; Scapoli, P.; and Spanedda, R. (1969). Chromosomes and chloramphenicol. Arch. Ital. Patol. Clin. Tumori, 12:117-141.
[18] E.H. Al-Neimy, Biological effects of Capparis spinosa and Rumex acetosella extracts on animal and human normal and tumor cells. Ph.D. thesis, college of science, 2007, Al-Nahrain University.
[19] R. Chatterjee, G. A. Haines, D. M. Perera, A. Goldston, and L. D. Morris. Testicular and sperm DNA damage after treatment with fludarabin for chronic lymphocytic leukemia Hum. Reprod. 2000, 15: 762-766.
[20] Z. y. Wang, S. J. Chen, Z. C. Zhon, M. Athar, W. A. Khan, D .R. Bickers, and H. Muthtar. Antimutagenic activity of green tea polyphenols. Mut. Res., 1989, 223: 273-285.