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Differentiation Capacity of Mouse L929 Fibroblastic Cell Line Compare With Human Dermal Fibroblast

Authors: Kasem Theerakittayakorn, Tanom Bunprasert


Mouse L929 fibroblastic cell line, which is widely used in many experiment aspects, was tested for their differentiation potency in osteogenic differentiation and adipogenic differentiation. Human dermal fibroblasts, which their differentiation potency are still be in confliction, also be taken in the experiment. The differentiations were conducted by using the inducing medium ingredients which is generally used to induce differentiation of stem cells. By the inducing media used, L929 mouse fibroblasts successfully underwent osteogenic differentiation and adipogenic differentiation while human dermal fibroblasts underwent only osteogenic differentiation but not for adipogenic differentiation. Human dermal fibroblasts are hard to be differentiated in adipogenic lineage and need specific proper condition for induction.

Keywords: osteogenic differentiation, fibroblast, Adipogenic differentiation, Inducingmedium

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[1] R. A. Evans, et al., "TGF-beta1-mediated fibroblast-myofibroblast terminal differentiation-the role of Smad proteins," Exp Cell Res, vol. 282, pp. 90-100, Jan 15 2003.
[2] C. Helary, et al., "Dense fibrillar collagen matrices: a model to study myofibroblast behaviour during wound healing," Biomaterials, vol. 27, pp. 4443-52, Sep 2006.
[3] D. Bi, et al., "Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters," BMC Cell Biol, vol. 11, p. 46, 2010.
[4] J. P. Junker, et al., "Adipogenic, chondrogenic and osteogenic differentiation of clonally derived human dermal fibroblasts," Cells Tissues Organs, vol. 191, pp. 105-18, 2010.
[5] S. B. Nicoll, et al., "Hyaluronidases and CD44 undergo differential modulation during chondrogenesis," Biochem Biophys Res Commun, vol. 292, pp. 819-25, Apr 12 2002.
[6] A. Sorisky, et al., "Evidence of adipocyte differentiation in human orbital fibroblasts in primary culture," J Clin Endocrinol Metab, vol. 81, pp. 3428-31, Sep 1996.
[7] F. Jeney, et al., "Cytochemical studies on the fibroblast-preadipocyte relationships in cultured fibroblast cell lines," Acta Histochem, vol. 102, pp. 381-9, Nov 2000.
[8] C. Shui and A. M. Scutt, "Mouse embryo-derived NIH3T3 fibroblasts adopt an osteoblast-like phenotype when treated with 1alpha,25- dihydroxyvitamin D(3) and dexamethasone in vitro," J Cell Physiol, vol. 193, pp. 164-72, Nov 2002.
[9] F. Bretagnol, et al., "The effect of sterilization processes on the bioadhesive properties and surface chemistry of a plasma-polymerized polyethylene glycol film: XPS characterization and L929 cell proliferation tests," Acta Biomater, vol. 4, pp. 1745-51, Nov 2008.
[10] M. C. Serrano, et al., "Transitory oxidative stress in L929 fibroblasts cultured on poly(epsilon-caprolactone) films," Biomaterials, vol. 26, pp. 5827-34, Oct 2005.
[11] R. Zange and T. Kissel, "Comparative in vitro biocompatibility testing of polycyanoacrylates and poly(D,L-lactide-co-glycolide) using different mouse fibroblast (L929) biocompatibility test models," European journal of pharmaceutics and biopharmaceutics vol. 44, pp. 149-157, 1997.
[12] G. Faria, et al., "Chlorhexidine-induced apoptosis or necrosis in L929 fibroblasts: A role for endoplasmic reticulum stress," Toxicol Appl Pharmacol, vol. 234, pp. 256-65, Jan 15 2009.
[13] M. Nordin, et al., "Effects of exposure period of acetylsalicylic acid, paracetamol and isopropanol on L929 cytotoxicity," Toxicol In Vitro, vol. 5, pp. 449-50, 1991.
[14] T. Taniguchi, et al., "Hydrogen peroxide-induced arachidonic acid release in L929 cells; roles of Src, protein kinase C and cytosolic phospholipase A2alpha," Eur J Pharmacol, vol. 546, pp. 1-10, Sep 28 2006.
[15] B. Roelofs, et al., "Acute activation of glucose uptake by glucose deprivation in L929 fibroblast cells," Biochimie, vol. 88, pp. 1941-6, Dec 2006.
[16] M. Cartwright, et al., "The human nerve growth factor gene: structure of the promoter region and expression in L929 fibroblasts," Brain Res Mol Brain Res, vol. 15, pp. 67-75, Sep 1992.
[17] H. Declercq, et al., "Isolation, proliferation and differentiation of osteoblastic cells to study cell/biomaterial interactions: comparison of different isolation techniques and source," Biomaterials, vol. 25, pp. 757-68, Feb 2004.
[18] J. Stern and W. H. P. Lewis, "The colorimetric estimation of calcium in serum with o-cresolphthalein complexone," Clinica Chimica Acta, vol. 2, pp. 576-580, 1957.
[19] T. J. Wu, et al., "Studies on the microspheres comprised of reconstituted collagen and hydroxyapatite," Biomaterials, vol. 25, pp. 651-8, Feb 2004.
[20] K. Jaager and T. Neuman, "Human Dermal Fibroblasts Exhibit Delayed Adipogenic Differentiation Compared with Mesenchymal Stem Cells," Stem Cells Dev, Jan 16 2011.