Intervention of Sambucus Nigra Polyphenolic Extract in Experimental Arterial Hypertension

Authors: Manuela Ciocoiu, Laur Badescu, Oana Badulescu, Magda Badescu

Abstract:

The research focuses on the effects of polyphenols extracted from Sambucus nigra fruit, using an experimental arterial hypertension pattern, as well as their influence on the oxidative stress. The results reveal the normalization of the reduced glutathion concentration, as well as a considerable reduction in the malondialdehide serum concentration by the polyphenolic protection. The rat blood pressure values were recorded using a CODATM system, which uses a non-invasive blood pressure measuring method. All the measured blood pressure components revealed a biostatistically significant (p<0.05) blood pressure drop between the AHT and the AHT+P groups. The results prove that oxidative stress is considerably lower, statistically speaking, in rats with hypertension but also provided with natural polyphenolic protection from Sambucus nigra fruits than in the rats belonging to the control group. In addition to the demonstrated antioxidant effects, natural polyphenols also have other biological properties that might contribute to the cardioprotective effects.

Keywords: Arterial hypertension, Oxidative stress, Sambucus nigra

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1055287

References:

[1] K. D. Setchell, Phytoestrogens: the biochemistry, physiology, and implications for human health of soy isoflavones. Am J Clin Nutr. 68, 1998, pp. 1333S - 1346S.
[2] J. Burns, Relationship among antioxidant activity, vasodilatation capacity, and phenolic content of red wine. J Agric and Food Chemistry 48, 2000, pp. 220 - 230.
[3] L. Z. Tosca, M. L. Fernandez, Cardioprotective effects of dietary polyphenols. Recent Advances in Nutritional Sciences 135, 2005, pp. 2291 - 2295.
[4] V. Cheynier, Polyphenols in foods are more complex than often thought. Am J Clin Nutr 81, 2005, pp. 223S - 229S.
[5] L. H. Yao, Y. M Jiang, J. Shi, F. A. Tomas-Barberan, N. Datta, R. Singanusong, Flavonoids in food and their health benefits. Plant Foods Hum Nutr, 59, 2004, pp. 113 - 122.
[6] S. Pascual-Teresa, M. T. Sanchez -Ballesta, Anthocyanins: from plant to health. Phytochem Rev, 7, 2008, pp. 281 - 299.
[7] L. I. Mennen, D. Sapinho, A. De Bree, Consumption of foods rich in flavonoids is related to a decreased cardiovascular risk in apparently healthy French women. J Nutr 134, 2004, pp. 923 - 926.
[8] D. R. Bell, K. Gochenaur, Direct vasoactive and vasoprotective properties of anthocyanin - rich extracts. J Appl Physiol 100, 2006, pp. 1164 - 1170.
[9] E. Beutler, O. Durion, B. J. Kelly, Diabetic heart and kidney exhibit increased resistance to lipid peroxidation. Biochem Biophys Acta 1047, 1990, pp. 63 - 69.
[10] H. Ohkawa, N. Ohisin, K. Yadik, Assay for lipid peroxides in animals tissues by thiobarbituric acid reaction. Annal Biochem 95, 1979, pp. 351 - 358.
[11] F. Nielsen, B. B. Mikkelsen, J. B. Nielsen, H. R. Andersen, P. Grandjean, Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem 43(7), 1997, pp. 1209 - 1214.
[12] D. Steinberg, S. Parthasarthy, T. E. Carew, J. C. Khoo, J. L. Witztum, Beyond cholesterol: modifications of low-density lipoprotein that increase its atherogenicity. N Engl J Med 320, 1989, pp. 915 - 924.
[13] E. Anselm, M. Chataigneau, M. Ndiaye, T. Chataigneau, V. B. Schini- Kerth, Grape juice causes endothelium-dependent relaxation via a redox-sensitive Src- and Akt-dependent activation of eNOS. Cardiovasc Res 73, 2007, pp. 404 - 413.
[14] J. E. Whittier, Y. Xiong, M. C. Rechsteiner, T. C. Squier, Hsp90 enhances degradation of oxidized calmodulin by the 20 S proteasome. J Biol Chem 279, 2004, pp. 46135 - 46142.
[15] T. C. Squier, Redox modulation of cellular metabolism through targeted degradation of signaling proteins by the proteasome. Antioxid Redox Signal 8, 2006, pp. 217 - 228.
[16] Y. Y. Lee-Hilz, A. M. Boerboom, A. H. Westphal, W. J. Berkel, J. M. Aarts, I. M. Rietjens, Pro-oxidant activity of flavonoids induces EpRE mediated gene expression. Chem Res Toxicol 19, 2006, pp. 1499 - 1505.
[17] I. Rahman, S. K. Biswas, P. A. Kirkham, Regulation of inflammation and redox signaling by dietary polyphenols. Biochem Pharmacol 72, 2006, pp. 1439 - 1452.
[18] E. Hernandez-Montes, S. E. Pollard, D. Vauzour, L. Jofre-Montseny, C. Rota, G. Rimbach, Activation of glutathione peroxidase via Nrf1 mediates genistein's protection against oxidative endothelial cell injury. Biochem Biophys Res Commun 346, 2006, pp. 851 - 859.
[19] R. Masella, R. Di Benedetto, R. Vari, C. Filesi, C. Giovannini, Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes. J Nutr Biochem 16, 10, 2005, pp. 577 - 586.
[20] M. Wro'blewska, J. Jus'kiewicz, S. Frejnagel, J. Oszmian'ski, Z. Zdun'czyk, Physiological influence of chokeberry phenolics in model diet. Acta Aliment Hung 37, 2008, pp. 221 - 232.