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Preliminary Toxicological Evaluations of Polypeptide-K Isolated from Momordica Charantia in Laboratory Rats
Abstract:This study examined the toxicological effects and safety of polypeptide k isolated from the seeds of Momordica charantia in laboratory rats. 30 male Sprague Dawley rats (12 weeks old, bodyweight 180-200 g) were randomly divided into 3 groups (1000 mg/kg, 500 mg and 0 mg/kg). Rats were acclimatized to laboratory conditions for 7 days and at day 8 rats were dosed orally with polypeptide k (in 2% DMSO/normal saline) and the controls received the dosed vehicle only. Rats were then observed for 72 hours before sacrificed. Rats were anaesthetized by pentobarbital (50 mg/kg ip) and 2-3.0 mL of blood was taken by cardiac puncture and rats were scarified by anaesthetic overdose. Immediately, organs (heart, lungs, liver, kidneys) were weigh and taken for histology. Organ sections were then evaluated by a histopathologist. Serum samples were assayed for liver functions (ALT and γ-GT) and kidney functions (BUN and creatinine). All rats showed normal behavior after the dosing and no statistical changes were observed in all blood parameters and organ weight. Histological examinations revealed normal organ structures. In conclusion, dosing of rats up to 1000 mg/kg did not have any effects on the rat behavior, liver or kidney functions nor histology of the selected organs.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1083417Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1621
 O. Ahmed, I., Adeghate, E., Sharma, A.K., Pallot, D.J., Singh, J., 1998. Effects of Momordica charantia fruit juice on islet morphology in the pancreas of the streptozotocin-diabetic rat. Diabetes Research Clinical Practices 40, 145-151.
 Modak, M., Dixit, P., Londhe, J., Ghaskadbi, S., Devasagayam, T. 2007. Indian Herbs and Herbal Drugs Used for the Treatment of Diabetes. Journal of Clinical Biochemistry & Nutrition 40: 163-173.
 Basch, E., Gabardi, S., Ulbricht, C., 2003. Bitter melon (Momordica charantia): a review of efficacy and safety. American Journal of Health and Systemic Pharmacology 65, 356-359.
 Grover, J.K., Yadav, S.P. 2004. Pharmacological actions and potential uses of Momordica Charantia: A Review. Journal of Ethnopharmacology 93: 123-124.
 Akhtar, M.S., 1982. Trial of Momordica charantia Linn (karela) powder in patients with maturity-onset diabetes. Journal of Pakistan Med Association 32, 106-107.
 Jayasooriya, A.P., Sakono, M., Yukizaki, C., Kawano, M., Yamamoto, K., Fukuda, N., 2000. Effects of Momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol free and cholesterol-enriched diets. Journal of Ethnopharmacology 72, 331-336.
 Grover, J.K., Vats, V., Rathi, S.S., Dawar, R., 2001. Traditional Indian antidiabetic plants attenuate progression of renal damage in streptozotocin induced diabetic mice. Journal of Ethnopharmacology 76, 233-238.
 Pari, L., Ramakrishnan, R., Venkateswaran, S., 2001. Antihyperglycaemic effect of Diamed, a herbal formulation, in experimental diabetes in rats. Journal of Pharmacy Pharmacology 53, 1139-1143.
 Virdi, J., Sivakami, S., Shahani, S., Suthar, A.C., Banavalikar, M.M., Biyani, M.K., 2003. Antihyperglycemic effects of three extracts from Momordica charantia. Journal of Ethnopharmacology 88, 107-111.
 Khanna, P., Jain, S.C., Panagariya, A. 1981. Hypoglycemic activity of polypeptide-p from a plant source. Journal of Natural Products 44: 648- 655.
 Matsuda, H., Li, Y., Murakami, T., Matsumura, N., Yamahara, J., Yoshikawa, M., 1998. Antidiabetic principles of natural medicines. Part III. Structure-related inhibitory activity and action mode of oleanolic acid glycosides on hypoglycemic activity. Chemical and Pharmaceutical Bulletin (Tokyo) 46, 1399-1403.
 Kanna, P. 2004. Protein/polypeptide-k obtained from Momordica charantia. United States Patent 6831162.
 Kusamran, W.R., Ratanavila, A., Tepsuwan, A., 1998. Effects of neem flowers, Thai and Chinese bitter gourd fruits and sweet basil leaves on hepatic monooxygenases and glutathione S-transferase activities, and in vitro metabolic activation of chemical carcinogens in rats. Food and Chemical Toxicology 36, 475-484.
 Somchit, N., Norshahida, A. R., Hasiah, A. H., Zuraini, A., Sulaiman, M. R., Noordin, M. M., Hepatotoxicity induced by antifungal drugs itraconazole and fluconazole in rats: a comparative in vivo study, Human and Experimental Toxicology, vol. 23, no. 11, pp. 519-525, 2004.
 Tennekoon KH, Jeevathayaparan S, Angunawala P et al. Effect of Momordica charantia on key hepatic enzymes. J Ethnopharmacol. 1994; 44:93-7.
 Yuan, X., Gu, X., Tang, J. 2008. Purification and characterisation of a hypoglycemic peptide from Momordica Charantia L. Var. abbreviate Ser. Food Chemistry 111: 415-420.
 Baldwa, V.S., Goyal, R.K., Bhandari, C.M., Pangariya, A., 1976. A clinical trial of insulin obtained from the vegetable source (plant insulin) in patients with diabetes mellitus. Rajasthan Medical Journal 16, 54.
 Lee-Huang, S., Huang, P.L., Chen, H.C., Huang, P.L., Bourinbaiar, A., Huang, H.I., Kung, H.F., 1995a. Anti-HIV and anti-tumor activities of recombinant MAP30 from bitter melon. Gene 61, 151-156.