Detection of Salmonella in Egg Shell and Egg Content from Different Housing Systems for Laying Hens

Authors: Wiriya Loongyai, Kiettisak Promphet, Nilubol Kangsukul, Ratchawat Noppha

Abstract:

Polymerase chain reaction (PCR) assay and conventional microbiological methods were used to detect bacterial contamination of egg shells and egg content in different commercial housing systems, open house system and evaporative cooling system. A PCR assay was developed for direct detection using a set of primers specific for the invasion by A gene (invA) of Salmonella spp. PCR detected the presence of Salmonella in 2 samples of shell egg from the evaporative cooling system, while conventional cultural methods detected no Salmonella from the same samples.

Keywords: egg content, egg shell, invA gene, PCR, Salmonellaspp.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1082740

References:

[1] V.K. Sharma, S.A. Carlson, Simultaneous detection of Salmonella strains and Escherichia coli O157:H7 with fluorogenic PCR and singleenrichment- broth culture, Appl. Environ. Microbiol, Vol. 66, pp. 5472- 5476, Sep, 2000.
[2] W.A. Gebreyes, P.R. Davies, W.E.M. Morrow, J.A. Funk, C. Altier, Antimicrobial resistance of Salmonella isolates from swine, J. Clin. Microbiol, Vol. 38, pp. 4633-4636, Sep, 2000.
[3] G. Rajashekara, E. Harverly, D.A. Halvorson, K.E. Ferris, D.C. Lauer, K.V. Nagaraja, Multidrug resistant Salmonella typhimurium DT104 in poultry, J. Food Prot, Vol. 63, pp. 155-161, Feb, 2000.
[4] F. Akhtar, H. Afzal, M. Ashfaque, Z. Nasim, Egg-shell contaminations of human importance with reference to salmonellae, Pak. Vet. J., Vol. 2, No. 1, pp. 10-11, Jan, 1982.
[5] F.J. Mayes, M.A.Takeballi, Microbial contamination of the hen-s egg: a review, J. Food Prot., Vol. 46, No. 12, pp. 1092-1098, Mar, 1983..
[6] F.T. Jones, D.V. Rives, J.B. Carey, Salmonella contamination in commercial eggs and an egg production facility, Poultry Sci., Vol. 74, No. 4, pp.753-757,Apr, 1995.
[7] M. M. Rahman, Z. U. M. Khan, A. N. M. Rahman, M. S. Islam, Recovery of Salmonella from Incubated eggs, newly hatched chicks and contaminated environments, J. Anim. Vet. Adv., Vol. 5, No. 7, pp. 598- 601, Jul, 2006.
[8] K. Iida, A. Abe, H. Matsui, H. Danbara, S. Wakayama, K. Kawahara, Rapid and sensitive method for detection of Salmonella strains using a combination of polymerase chain reaction and reverse dot-blot hybridization, FEMS Microbiol. Lett., Vol. 114, pp. 167-172, Sep, 1993.
[9] J. L. Doran, S. K. Collinson, J. Burian, G. Sarlos, E. C. D. Todd, C. K. Munro, C. M. Kay, P. A. Banser, P. I. Peterkin, W. W. Kay, DNA-based diagnostic test for Salmonella species targeting agfA, the structural gene for thin aggregative fimbriae, J. Clin. Microbiol., Vol. 31, pp. 2263- 2273, Sep, 1993.
[10] Y. Hashimoto, Y. Itho, Y. Fujinaga, A. Q. Khan, F. Sultana, M. Miyake, K. Hirose, H. Yamamoto, T. Ezaki, Development of nested PCR based on the ViaB sequence to detect Salmonella typhi, J. Clin. Microbiol., Vol. 33, pp. 775-777, Nov, 1995.
[11] J. Mahon, A. Lax, A quantitative polymerase chain reaction method for the detection in avian faeces of salmonellas carrying the spvR gene, Epidemiol. Infect., Vol. 111, pp. 455-464, June, 1993.
[12] K. Rahn, S. A. De Grandis, R. C. Clarke, S. A. McEwen, J. E. Galan, C. Ginocchio, R. Curtiss, C. L. Gyles, Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella, Mol. Cell. Probes., Vol. 6, pp. 271-279, Aug, 1992.
[13] M. BÜlte, P. Jakob, The use of a PCR-generated invA probe for the detection of Salmonella spp. in artificially and naturally contaminated foods, Int. J. Food Microbial., Vol. 26, pp. 335-344, Aug, 1995.
[14] H. Wang, B. W. Blais, H. Yamazaki, Rapid confirmation of polymyxincloth enzyme immunoassay for group D salmonellae including Salmonella enteritidis in eggs by polymerase chain reaction, Food Control., Vol. 6, pp. 205-209, Aug, 1995.
[15] J. Mahon, C.K. Murphy, P.W. Jones, P.A. Barrow, Comparison of multiplex PCR and standard bacteriological methods of detecting Salmonella on chicken skin, Lett. Appl. Microbiol., Vol. 19, pp. 169- 172. Apr, 1994.
[16] L. R. Santos, V. P. Nascimento, S. D. Oliveira, M. L. Flores, A. P. Pontes, A. R. Ribeiro, C. T. P. Salle, R. F. F. Lopes, Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat, Rev. Inst. Med. Trop. Sao. Paulo., Vol. 43, No. 5, pp. 247- 250, Oct, 2001.
[17] I.S. Schrank, M. A. Z. Mores, J. L. A. Costa, A. P. G. Frazzon, R. Soncini, A. Schrank, M.H. Vainstein, S. C. Silva, Influence of enrichment media and application of a PCR based method to detect Salmonella in poultry industry products and clinical samples, Vet. Microbiol., Vol. 82, pp. 45-53, Sep, 2001.
[18] A. Rozila, A.W. Hartini, D.Y. Tan, S. K. Wee, Rapid Molecular Detection of Salmonella Isolated from Poultry Farm. The 19th Veterinary Association Malaysia Congress, 3 - 5 August 2007, pp. 201 - 203, 2007.
[19] M. A. G. Robles, A. M. Loredo, G. A. Ojeda, J. A. O. Garc'ia, I. O. Mart'inez, L. H. M. Ramos, P. Fratamico, PCR Detection and Microbiological Isolation of Salmonella spp. from Fresh Beef and Cantaloupes, J. Food. Sci., Vol. 74, No. 1, pp. 37-40, Jan, 2009.