Effect of COD Loading Rate on Hydrogen Production from Alcohol Wastewater

Authors: Patcharee Intanoo, Jittipan Chavadej, Sumaeth Chavadej

Abstract:

The objective of this study was to investigate hydrogen production from alcohol wastewater by anaerobic sequencing batch reactor (ASBR) under thermophillic operation. The ASBR unit used in this study had a liquid holding volume of 4 L and was operated at 6 cycles per day. The seed sludge taken from an upflow anaerobic sludge blanket unit treating the same wastewater was boiled at 95 °C for 15 min before being fed to the ASBR unit. The ASBR system was operated at different COD loading rates at a thermophillic temperature (55 °C), and controlled pH of 5.5. When the system was operated under optimum conditions (providing maximum hydrogen production performance) at a feed COD of 60 000 mg/l, and a COD loading rate of 68 kg/m3 d, the produced gas contained 43 % H2 content in the produced gas. Moreover, the hydrogen yield and the specific hydrogen production rate (SHPR) were 130 ml H2/g COD removed and 2100 ml H2/l d, respectively.

Keywords: Biohydrogen, Alcohol wastewater, Anaerobic sequencing batch reactor (ASBR), Thermophillic operation

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1082399

References:

[1] Das D, Veziroglu TN. "Hydrogen production by biological processes: a survey of literature". International Journal Hydrogen Energy, Vol. 26, pp. 13-28, 2001.
[2] Lay JJ, Le YJ, Noike T. "Feasibility of biological hydrogen production from organic fraction of municipal solid waste". Water Research, Vol. 33, pp. 2579-2586, 1999.
[3] Mizuno O, Dinsdale R, Hawkes DL, Noike T. "Enhancement of hydrogen production from glucose by nitrogen gas sparging". Bioresource Technology, Vol. 73, pp.59-65, 2000.
[4] Kapdan IK, Kargi F. "Bio-hydrogen production from waste materials". Enzyme Microbial Technology, Vol. 38, pp. 569-582, 2006.
[5] Mohan SV, Babu VL, Sarma PN. "Anaerobic biohydrogen production from dairy wastewater treatment in sequencing batch reactor (AnSBR): effect of organic loading rate". Enzyme Microbial Technology, Vol. 41, pp. 506-15, 2007.
[6] Han SK, Shin HS. "Biohydrogen production by anaerobic fermentation of food waste". International Journal Hydrogen Energy, Vol. 29pp. 569- 577, 2004.
[7] Sreethawong T, Chatsiriwatana S, Rangsunvigit P, Chavadej S. "Hydrogen production from cassava wastewater using an aerobic sequencing batch reactor: Effects of operational parameters, COD:N ratio, and organic acid composition". International Journal Hydrogen Energy, Vol. 35, pp. 4092-4102, 2010.
[8] Shin HS, Youn JH, Kim SH. "Hydrogen production from food waste in anaerobic mesophillic and thermophillic acidogenesis". International Journal Hydrogen Energy, Vol. 29, pp. 1355-1363. 2004.
[9] Arooj MF, Han SK, Kim AH, Kim DH, Shin HS. "Effect of HRT on ASBR converting starch into biologicl hydrogen". International Journal Hydrogen Energy, Vol. 33, pp. 6509-6514, 2008.
[10] Argun H, Kargi F, Kapdan IK, Oztekin R. "Biohydrogen production by dark fermemtation of wheat powder solution: Effects of C/N and C/P ratio on hydrogen yield and formation rate". International Journal Hydrogen Energy, Vol. 33, pp. 1813-1819, 2008.
[11] Chong ML, Sabaratnam V, Shirai Y, Hassan MA. "Biohydrogen production from biomass and industrial wastes by dark fermentation". International Journal Hydrogen Energy, Vol. 34, pp. 3277-3287, 2009.
[12] Lee KS, Hsu YF, Lo YC, Lin PJ, Lin CY, Chang JS. "Exploring optimal environmental factors for fermentative hydrogen production from starch using mixed anaerobic microflora". International Journal Hydrogen Energy, Vol. 32, pp. 1565-1572, 2008.
[13] Wang J, Wan W. "Comparison of different pretreatment methods for enriching hydrogen-producing bacteria from digested sludge". International Journal Hydrogen Energy, Vol. 33, pp. 2934-2941, 2008.
[14] Hawkes FR, Hussy I, Kyazze G, Dinsdale R, Hawkes DL. Continuous dark fermentative hydrogen production by mesophilicmicroflora: Principles and progress. International Journal Hydrogen Energy, Vol. 32, pp. 174-184, 2007.
[15] Lin CY, Hung WC. "Enhancement of fermentative hydrogen/ethanol production from cellulose using mixed anaerobic cultures". International Journal Hydrogen Energy, Vol. 33, pp. 3660-3667, 2008.
[16] Kapdan IK, Kargi F. "Bio-hydrogen production from waste materials". Enzyme Microbial Technology, pp. 38569-53882, 2006.
[17] Cheng J, Su H, Zhou J, Song W, Cen K. "Hydrogen production by mixed bacteria through dark and photo fermentation". International Journal Hydrogen Energy, Vol. 36, pp. 450-457, 2011.
[18] Hwang JH, Choi JA, Shanab RA, Bhatnagar A, Min B, Song H, Kumar E, Choi J, Lee ES, Kim YJ, Umg S, Lee DS, Joen BH. "Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora". International Journal Hydrogen Energy, Vol. 34, pp. 9702- 9719, 2009.
[19] Eaton AD, Clesceri LS, Rice EW, Greenberg AE, "Standard Methods for The Examination of Water and Wastewater". American Public Health Association (APHA), American Water Works Association (AWWA) & Water Environment Federation (WEF), Washington, DC, 2005.
[20] Sreethawong T, Niyamapa T, Neramitsuk H, Rangsunvigit P, Leethochawalit M, Chavadej S. "Hydrogen production from glucosecontaining wastewater using an anaerobic sequencing batch reactor: Effects of COD loading rate, nitrogen content. And organic acid composition". Chemical Engineering Journal, Vol. 160, pp. 322-332, 2010.
[21] Fang HH, Liu H. "Effect of pH on hydrogen production from glucose by a mixed culture". Bioresource Technology, Vol. 82, pp. 87-93, 2002.
[22] Lin CY, Lay CH. "Effects of carbonate and phosphate concentrations on hydrogen production using anaerobic sewage sludge microflora". International Journal Hydrogen Energy, Vol. 29, pp. 275-281, 2004.
[23] Chen WH, Sung S, Chen SY. "Biological hydrogen production in an anaerobic sequencing batch reactor: pH and cyclic duration effects". International Journal Hydrogen Energy, Vol. 34, pp. 227-234, 2009.
[24] Miyake J. "The science of biohydrogen: an energetic view. In: Zaborsky OR, editor. Biohydrogen". New York: Plenum Press, pp. 7-18, 1998.
[25] Luo G, Xie L, Zou Z, Zhou Q, Wang JY. "Fermentative hydrogen production from cassava stillage by mixed anaerobic microflora: Effects of temperature and pH". Applied Energy, Vol. 87, pp. 3710-3117, 2010.
[26] O-Thong S, Prasertsan P, Intrasungkha, Dhamwichukorn S, Birkeland NK. "Optimization of simultaneous thermophillic fermentative hydrogen production and COD reduction from palm oil mill effluent by Thermoanaerobacterium-rich sludge". International Journal Hydrogen Energy, Vol. 33, pp. 1221-31, 2008.
[27] Hawkes FR, Dinsdale R, awkes DL, Hussy I. "Sustainable fermentative hydrogen production: challenges for process optimization". International Journal Hydrogen Energy, Vol. 27, pp. 1339-1347, 2002.
[28] Guo WQ, Ren NQ, Wang XJ, Xiang WS, Meng ZH, Ding J, Qu YY, Zhang LS. "Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor". International Journal Hydrogen Energy, Vol. 33, pp. 4981-4988, 2008.