Evaluation of Chlorophyll Content and Chlorophyll Fluorescence Parameters and Relationships between Chlorophyll a, b and Chlorophyll Content Index under Water Stress in Olea europaea cv. Dezful

Authors: E. Khaleghi, K. Arzani, N. Moallemi, M. Barzegar

Abstract:

This study was conducted to determine effect of water stress on chlorophyll content and chlorophyll fluorescence parameter in young `Dezful- olive trees. Three irrigation regimes (40% ETcrop, 65% ETcrop and 100% ETcrop) were used. After irrigation treatments were applied, some of biochemical parameters including chlorophyll a, b, total chlorophyll, chlorophyll fluorescence and also chlorophyll content index (C.C.I) were measured. Results of Analysis of variance showed that irrigation treatments had significant effect on chlorophylla, total chlorophyll (chl a+b), C.C.I and Fv/Fm ratio. The amount of decreased chlorophyll a and total chlorophyll in plants were received 40% ETcrop were 51.55% and 46.86%, respectively, compared with 100% ETcrop.

Keywords: Evatarnspiration (ETcrop), Chlorophyll Content, Chlorophyll Fluorescence, Water stress, Olive

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1082211

References:

[1] S. Falk, D. P. Maxwell, D. E. Laudenbach, N. P.A. Huner, and N. R. Baker, "In Advances in Photosynthesis, V.5, Photosynthesis and the Environment". Kluwer Academic Publishers, Dordrecht Boston London. 1996, pp. 367-385.
[2] J.L. Araus, T. Amaro, J. Voltas, H. Nakkoul, and M.M Nachit, M M, "Chlorophyll fluorescence as a selection criterion for grain yield in durum wheat under Mediterranean conditions". Field Crops Research, 1998, Vol. 55, pp.209-223.
[3] P. Guo, and R. Li, Effects of high nocturnal temperature on photosynthetic organization in rice leaves". Acta Botanica Sinica, 2000, Vol. 42, pp. 13-18.
[4] Y. Fracheboud, C. Jompuk, J. M. Ribaut, P. Stamp, and J. Leipner, "Genetic analysis of cold-tolerance of photosynthesis in maize". Plant Molecular Biology, 2004. Vol. 56, pp.241-253.
[5] K. Gregoriou, K. Pontikis, and S. Vemmos, "Effects of reduced irradiance on leaf morphology, photosynthetic capacity, and fruit yield in olive (Olea europaea L.)". Photosynthetica, 2007, Vol.45, No.2, pp.172-181.
[6] F. Loreto, M. Centritto, and K. Chartzoulakis, "Photosynthetic limitations in olive cultivars with different sensitivity to salt stress". Plant Cell Environ, 2003, Vol. 26, pp. 595-60 .
[7] J .C. Melgar, L. Guidi, D. Remorini, G. Agati, E. Degli-innocenti, S. Castelli, M. C. Baratto, C. Faraloni, and M. Tattini, "Antioxidant defences and oxidative-damage in salt-treated olive plants under contrasting sunlight irradiance". Tree Physiol., 2009 29: 1187-1198.
[8] J. D. Hiscox, and G.F. Israelstam, " A method for the extraction of chlorophyll from leaf tissue without maceration". Canadian Journal of Botany, 1979, Vol. 57, pp.1332-1334.
[9] D.I, Arnon, "Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris". Plant Physiology, 1949, Vol. 24, pp.1-15.
[10] G. H. Krause, and E. Weis, "Chlorophyll fluorescence and photosynthesis: the basics". Annu Rev Plant Physiol Plant Mol Biol., 1991. Vol. 42, pp. 313-349.
[11] F. Anjum, M. Yaseen, E. Rasul, A. Wahid, and S. Anjum, "Water stress in barley (Hordeum vulgare L.). II. Effect on chemical composition and chlorophyll contents". Pakistan J Agric Sci., 2003. Vol.40, pp. 45-49.
[12] M. Farooq, A. Wahid, N. Kobayashi, D. Fujita, and S. M. A. Basra, ÔÇÿPlant drought stress: effects, mechanisms and management". Agron Sustain Dev., 2009, Vol. 29, pp. 185-212
[13] A. Massacci,S. M. Nabiev, L. Pietrosanti, S. K. Nematov, T.N. Chernikova, K. Thor, and J. Leipner, "Response of the photosynthetic apparatus of cotton (Gossypium hirsutum) to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging". Plant Physiol Biochem., 2008, Vol. 46, pp.189-195
[14] S. P. Kiani, P. Maury, A. Sarrafi, and P. Grieu, “QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annuus L.) under well-watered and water-stressed conditions”. Plant Sci., 2008, Vol. 175, pp. 565–573
[15] M. Guerfel, O. Baccouri, D. Boujnah, W. Chaı¨bi, and M. Zarrouk, “Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olives (Olea europaea L.) cultivars”. Scientia Horticulturae, 2009a, Vol.119, pp. 257–263.
[16] I. Arji, and K. Arzani, “Effect of water stress on some biochemical changes in leaf of five olive (Olea europaea L.) cultivars”. Acta Horticulturae, 2008, Vol. 791, pp. 523-526.
[17] K. Arzani, and N. Yazdani, “The influence of drought stress and paclobutrazol on quantitative changes of proteins in olive (Olea europaea L.) cultivars `Bladi' and `Mission'”. Acta Horticulturae , 2008, Vol.791, pp.527-530.
[18] I. Arji, K. Arzani, H. Ebrahimzadeh, and R. Asghari, “Effect of Drought stress on Physiological, morphological and biochemical characteristics of some olive cultivars”. Thesis submitted in partial fulfillment of the requirement for the degree of doctor of philosophy in Horticulture Science, Agriculture Engineering. Tarbiat Modares University, Tehran. Iran. 2003.
[19] M. Guerfel, Y. Ouni, D. Boujnah, and M. Zarrouk, “Photosynthesis parameters and activities of enzymes of oxidative stress in two young ‘Chemlali’ and ‘Chetoui’ olive trees under water deficit”. Photosynthetica, 2009b, Vol. 47, No.3, pp.340-346.
[20] B. Demming-Adams , W. W. Adams, D. H. Barker, B. A. Logan, D. R. Bowling, and A. S. Verhoven, “Using Chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation”. Physiol. Plant, 1993, Vol. 98 pp. 253–264.
[21] E. A. Bacelar, “Ecophysiological responses of olive (Olea europaea L.) to restricted water availability: Limitations, damages and drought resistance mechanisms”. Universidade De Trás-Os-Montes E Alto Douro Vila Real, 2006.
[22] C. Faraloni, I. Cutino, R. Petruccelli, A. R. Leva, S. Lazzeri, and G. Torzillo,“Chlorophyll fluorescence technique as a rapid tool for in vitro screening of olive cultivars” (Olea europaea L.) tolerant to drought stress”. Environmental and Experimental Botany, 2011, Vol.73, pp.49- 56.
[23] T. M. Cate, and T. D. Perkins,” Chlorophyll content monitoring in sugar maple (Acer saccharum)”. Tree Physiol., 2003, Vol. 23 No.15, pp.1077– 1079.
[24] B. L. Ma, and L. M. Dwyer, “Determination of nitrogen status in maize senescing leaves”. J. Plant Nutr., 1997, Vol. 20, pp. 1–8.
[25] L. M. Dwyer, A. M. Anderson, B. L. Ma, D. W. Stewart, M. Tollenaar and E. Gregorich, “Quantifying the nonlinearity in chlorophyll meter response to corn leaf nitrogen concentration”. Can J Plant Sci., 1994, Vol. 75, pp.179–182.
[26] D. W. Reeves, P.L Mask, C. W. Wood, and D. P. Delaney, “Determination of wheat nitrogen status with a hand-held chlorophyll meter: influence of management practices”. J Plant Nutr., 1993, Vol.16, pp.781–796.
[27] R.J. Campbell, K. N. Mobley, R. P. Marini, and D. G. Pfeiffer “ Growing conditions alter the relationship between SPAD-501 values and apple leaf chlorophyll”. Hortscience, 1990, Vol.25, pp.330–331.
[28] M. Ghasemi, K. Arzani, A. Yadollahi, S. Ghasemi, and S. Sarikhani Khorrami, “Estimate of Leaf Chlorophyll and Nitrogen Content in Asian Pear (Pyrus serotina Rehd.) by CCM-200”. Not Sci Biol.,2011, Vol.3, No.1. pp.:91-94.
[29] D. Bussis, F. Kauder, and D. Heineke, “ Acclimation of potato plants to polyethylene glycol-induced water deficit. I. Photosynthesis and metabolism”. J Exp Bot., 1998, Vol. 49, pp.1349–1360.
[30] N. Smirnoff, “The role of active oxygen in the response of plants to water deficit and desiccation”. New Phytol., 1993, Vol.125, pp.27–58.
[31] C. H. Foyer, and G. “Noctor, Oxygen processing in photosynthesis: regulation and signaling”. New Phytol., 2000, Vol. 146, pp. 359-388.
[32] M. E. Younis, O. A. El-Shahaby, S. A. Abo-Hamed, and A. H. Ibrahim,“Effects of water stress on growth, pigments and 14 CO2 assimilation in three sorghum cultivars”. Agron Crop Sci., 2000, Vol. 185, No. 2, pp: 73–82.
[33] J.F. Moran, M. Becan, I. Iturbe-Ormaetyl, S. Frechilla, R. V. Klucas, and P. Aparicio-Tejo, “Drought induces oxidative stresses in pea plants”. Planta, 1994, Vol.194, pp.346– 352.