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High Performance Liquid Chromatographic Method for Determination of Colistin Sulfate and its Application in Medicated Premixand Animal Feed
Abstract:The aim of the present study was to develop and validate an inexpensive and simple high performance liquid chromatographic (HPLC) method for the determination of colistin sulfate. Separation of colistin sulfate was achieved on a ZORBAX Eclipse XDB-C18 column using UV detection at λ=215 nm. The mobile phase was 30 mM sulfate buffer (pH 2.5):acetonitrile(76:24). An excellent linearity (r2=0.998) was found in the concentration range of 25 - 400 μg/mL. Intra- day and inter-day precisions of method (%RSD, n=3) were less than 7.9%.The developed and validated method was applied to determination of the content of colistin sulfate in medicated premix and animal feed sample.The recovery of colistin from animal feed was satisfactorily ranged from 90.92 to 93.77%. The results demonstrated that the HPLC method developed in this work is appropriate for direct determination of colistin sulfate in commercial medicated premixes and animal feed.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1082195Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 7365
 J. Orwa, A. Van Gerven, E. Roets, and J. Hoogmartens, "Development and validation of a liquid chromatography method for analysis of colistin sulphate," Chromatographia, vol 51, pp. 433-436, 2000.
 Y. Ikai, H. Oka, J. Hayakawa, N. Kawamura, K Harada, M.Suzuki, H.Nakazawa and Y. Ito, Journal of Liquid Chromatography and Related Technologies, vol 21, pp.143, 1998
 A.R. Martin, J.L. Delgado, and W.A. Remers (Eds.), Wilson and Gisvold-s Textbook of Organic Medicinal and Pharmaceutical Chemistry, 10th ed., 1998.
 Y. Xu, X.i Tian, C. Ren, H. Huang, X. Zhang, X. Gong, H. Liu, Z. Yu, and L. Zhang, "Analysis of colistin A and B in fishery products by ultra performance liquid chromatography with positive electrospray ionization tandem mass spectrometry," Journal of Chromatography B, vol 899,pp. 14-20, 2012.
 The notification of the Department of Livestock Development (No.2/2) , " the maximum residue limits of animal product", Department of Livestock Development, Bangkok, Thailand, May, 2001.
 S. Morales-Munoz and M. D. Luque de Castro, "Dynamic ultrasoundassisted extraction of colistin from feeds with on-line pre-column derivatization and liquid chromatography-fluorimetric detection," Journal of Chromatography A, vol 1066, pp. 1-7, 2005.
 P. Leroy, D. Decolin, S. Nicolas, P. Archimbault and A. Nicolas, "Residue determination of two co-administered antibacterial agents cephalexin and colistin in calf tissues using high-performance liquid chromatography and microbiological methods.," Journal of Pharmaceutical and Biomedical Analysis, vol 7, pp.1837-1846, 1989.
 T. Suzuki, H. Inouye, K. Fujikawa and Y. Suketa, "Studies on the Chemical Structure of Colistin: I. Fractionation, Molecular Weight Determination, Amino Acid and Fatty Acid Composition," Journal of Biochemistry, vol 54, pp.25-33 ,1963.
 A. H. Thomas and L. Holloway, "Thin-layer chromatographic method for the identification of the polymyxins," Journal of Chromatography A, vol 161, pp.417-420, 1978.
 W. Holska and E. Gwozdz.,"Determination of colistin by isotachophoresis," Journal of Liquid Chromatography, vol 12, pp. 2761- 67, 1989.
 H. K. Kristensen, and S. H. Hansen, "Separation of Polymyxins by Micellar Electrokinetic Capillary Chromatography," Journal of Chromatography, vol. 628, pp. 309-315, 1993.
 B. Cancho Grande, M. Garc├¡a Falc├│n, C. Pérez-Lamela, M. Rodr├¡guez Comesa├▒a, and J. Simal G├índara, "Quantitative analysis of colistin and tiamulin in liquid and solid medicated premixes by HPLC with diodearray detection," Chromatographia, vol 53, pp. 460-463, 2001.
 J. Li, R. W. Milne, R. L. Nation, J.D. Turnidge, K. Coulthard, and D. W. Johnson, "A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase highperformance liquid chromatography," Journal of Chromatography B: Biomedical Sciences and Applications, vol 761, pp.167-175, 2001.
 C. Cheng, S. Liu, D. Xiao, J. Hollembaek, L. Yao, J. Lin, and S. Hansel, "LC-MS/MS method development and validation for the determination of polymyxins and vancomycin in rat plasma," Journal of Chromatography B, vol 878, pp.2831-2838, 2010.
 D. W. Sin, E. C. Wan, C. Ho, and Y. C. Wong., "Detection of residual bacitracin A, colistin A, and colistin B in milk and animal tissues by liquid chromatography tandem mass spectrometry," Analytical and Bioanalytical Chemistry , vol 385, pp.181-188, 2006.