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Optimization of Two-Stage Pretreatment Combined with Microwave Radiation Using Response Surface Methodology

Authors: Apanee Luengnaruemitchai, Sujitra Wongkasemjit, Jidapa Manaso

Abstract:

Pretreatment is an essential step in the conversion of lignocellulosic biomass to fermentable sugar that used for biobutanol production. Among pretreatment processes, microwave is considered to improve pretreatment efficiency due to its high heating efficiency, easy operation, and easily to combine with chemical reaction. The main objectives of this work are to investigate the feasibility of microwave pretreatment to enhance enzymatic hydrolysis of corncobs and to determine the optimal conditions using response surface methodology. Corncobs were pretreated via two-stage pretreatment in dilute sodium hydroxide (2 %) followed by dilute sulfuric acid 1 %. Pretreated corncobs were subjected to enzymatic hydrolysis to produce reducing sugar. Statistical experimental design was used to optimize pretreatment parameters including temperature, residence time and solid-to-liquid ratio to achieve the highest amount of glucose. The results revealed that solid-to-liquid ratio and temperature had a significant effect on the amount of glucose.

Keywords: Pretreatment, response surface methodology, microwave radiation, Corncobs

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1081896

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