Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30172
Investigation of Anti-diabetic and Hypocholesterolemic Potential of Psyllium Husk Fiber (Plantago psyllium) in Diabetic and Hypercholesterolemic Albino Rats

Authors: Ishtiaq Ahmed, Muhammad Naeem, Abdul Shakoor, Zaheer Ahmed, Hafiz Muhammad Nasir Iqbal

Abstract:

The present study was conducted to observe the effect of Plantago psyllium on blood glucose and cholesterol levels in normal and alloxan induced diabetic rats. To investigate the effect of Plantago psyllium 40 rats were included in this study divided into four groups of ten rats in each group. One group A was normal, second group B was diabetic, third group C was non diabetic and hypercholesterolemic and fourth group D was diabetic and hypercholesterolemic. Two groups B and D were made diabetic by intraperitonial injection of alloxan dissolved in 1mL distilled water at a dose of 125mg/Kg of body weight. Two groups C and D were made hypercholesterolemic by oral administration of powder cholesterol (1g/Kg of body weight). The blood samples from all the rats were collected from coccygial vein on 1st day, then on 21st and 42nd day respectively. All the samples were analyzed for blood glucose and cholesterol level by using enzymatic kits. The blood glucose and cholesterol levels of treated groups of rats showed significant reduction after 7 weeks of treatment with Plantago psyllium. By statistical analysis of results it was found that Plantago psyllium has anti-diabetic and hypocholesterolemic activity in diabetic and hypercholesterolemic albino rats.

Keywords: Albino rats, alloxan, Plantago psyllium, statistical analysis

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1081743

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1708

References:


[1] D. W. Guthrie, and R. A. Guthrie, "Diabetes. The Diabetes Sourcebook. Los Angeles: Lowell House Publications., vol. 16, pp. 8-25, 1999.
[2] S. P. Wolff, "Diabetes mellitus and free radiacals," Br Med Bull., vol. 49, pp. 642-652, 1993.
[3] A. Robert, W. A. Wenatchee, F. L. Tallahassee, and S. R. Lynn, "Association of Diabetes with genetic & environmental factors," University of Maryland Medical Center. Diabetes., vol. 18 pp. 446-452, 1999.
[4] Anonymous, "Low Density lipoprotein as Bad Cholesterol," URL=http://en. wikipedia .org /wiki/low_density_lipoprotein., 2008.
[5] M. C. Garg, S. Ojha, and D. D. Bansal, "Anti-oxidant status of Streptozotocin in diabetic rats," Indian J Exp. Bio., vol. 34, pp. 264- 266, 1996.
[6] M. C. Sabu, and K. Ramadasan, "Anti-diabetic activity of medicinal plants and its relationship with their anti-oxidant property," J. Ethnopharmaco., vo. 81, pp. 155-160, 2002.
[7] L. Johadar, "Plants with hypoglycemic effects." Cesk farm., vol. 42, pp. 251-259, 1993.
[8] D. Sathishsekar, and S. Subramanian, "Beneficial effects of Momordica Charantia seeds in the treatment of STZ-induced diabetes in experimental rats," Biol. Pharm. Bull., vol. 28, pp. 978-983, 2005.
[9] K. G. Mehta, R. Modi, and R. Gupta, "Psyllium," Indian J Argon., vol. 21, pp. 509-510, 1976.
[10] R. N. Chopra, S. L. Nayar, and I. C. Chopra, "Glossary of Indian Medicinal Plants (including the supplement). New Delhi: Council of Scientific and Industrial Research. Vol. 43, pp. 56-67, 1986.
[11] Z. D. Abraham, and T. Mehta, "Three-week psyllium husk supplementation: Effect on plasma cholesterol concentrations, fecal steroid excretion, and carbohydrate absorption in men," Am J Clin Nutr., vol. 47, pp. 67-74, 1988.
[12] N. Washington, M. Harris, A. Mussellwhite, and R. C. Spiller, "Moderation of lactulose-induced diarrhea by psyllium: effects on motility and fermentation," Am. J Clin Nutr., vol. 7, pp. 317-321, 1998.
[13] M. L. Fernandez, "Soluble fiber and nondigestible carbohydrate effects on plasma lipids and cardiovascular risk," Curr Opin Lipidol., vol. 12, pp. 35-40, 2001.
[14] H. Terpstra, J. A. Lapre, H. T. Vares, and A. C. Beynen, "Hypocholestrolemic effect of dietry psyllium in female rats," Ann Nutr Metab., vol. 44, pp. 223-228, 2000.
[15] R. Burton, and V. Manninen, "Influence of a psyllium-based fibre preparation on feacal and serum parameters," Acta Med Scand., vol. 668, pp. 91-94, 1982.
[16] L. Romero, K. L. West, T. Zern, and M. L. Fernandez, "The seeds from P. ovata lower plasma lipid by altering hepatic and bile acid metabolism in guinea pigs," J Nutr., vol. 132, pp. 1194-1198, 2002.
[17] M. A. Pereira, O. E. Reilly, and K. Augusstsson, "Dietary fiber and risk of coronary heart disease. A pooled analysis of cohort studies," Arch Intern Med., vol. 164, pp. 370-376, 2004.
[18] L. Brown, B. Rosner, W. W. Willett, and F. M. Sacks, "Cholesterollowering effects of dietary fiber: a meta-analysis," Am. J. Clin Nutr., vol. 69, pp. 30-42, 1999.
[19] R. M. Krauss, R. H. Eckel, and B. Howard, "AHA dietary guidelines revision 2000 a statement for healthcare professionals from the Nutrition Committee of the American Heart Association,Circulation" vol. 102, pp. 2284-2299, 2000.
[20] G. Backer, E. Ambrosioni, and R. B. Johnsen, "European guidelines on cardiovascular disease prevention in clinical practice. Third Joint Task Force on European and other societies on Cardiovascular Disease Prevention in Clinical Practice," Eur Heart J., vol. 24, pp. 1601-1610, 2003.
[21] P. G. Reeves, F. H. Nielsen, and G. C. Fahey, "AIN-93 purified diets for laboratory rodents: final report of the American institute of nutrition Ad Hoc writing committee on reformulation of the AIN-76 rodent diet," J. Nutr., vol. 123, pp. 1939-1351, 1993.
[22] A. Junod, A. E. Lambert, W. Staufacher, and A. E. Renold, "Diabetogenic action of streptozotocin: relationship of dose to metabolic response," J Clin Invest., vol. 48, pp. 2129-2139, 1996.
[23] N. Vinuthan, S. Kanthasamy, S. William, S. Subramanian, and S. Govindasamy, "Insulinic action of vanadate on experimental diabetes," Pharmacol. Res., vol. 22, pp. 207-217, 2007.
[24] E. M. Mackey, and L. L. Macky "Estimation of serum glucose and cholesterol," J. Clin. Investigations., vol. 6, pp. 297, 1972.
[25] R. G. D. Steel, J. H. Torrie, and D. A. Dicky, "Principles and Procedure of Statistics. A biometrical approach," WCB McGraw Hill Book Co. Inc. New York. vol. 3, pp. 25-31, 1997.
[26] S. L. Abbate, and J. D. Brunzell, "Pathophysiology of hyperlipidemia in diabetes," J. cardiovascular pharmacol., vol. 16, pp. 1-7, 1990.
[27] M. Safdar, K. Alam, and Habibullah, "Effect of jaman fruit extract on serum, glucose and lipid profile in type -2 Diabetic Individuals," Pak. J. Nutr., vol. 5, pp. 573-576, 2006.
[28] F. J. Alarcon, R. R. Ramos, and S. P. Gutierrez, "Studyof the antihyperglycemic effect of plants used as anti-diabetics," J. Ethnopharmacol., vol. 61, pp. 101-110, 1998.
[29] S. A. Moharib, and S.A. Batran, "Hypoglycemic effect of dietary fiber in diabetic rats," Research J. Agri. Biol. Sci., vol. 4, pp. 455-461, 2008.
[30] M. M. Rodriguez, R. F. Guerrero, and B. G. Lazcano, "Lipid- and glucose-lowering efficacy of Plantago Psyllium in type II diabetes," J. Diabetes Complications., vol. 12, pp. 273-278, 1998.
[31] D. Kritchevsky, S. A. Tepper, and D. M. Klurfeld, "Influence of psyllium preparations on plasma and liver lipids of cholesterol-fed rats," Artery" vol. 21, pp. 303-11, 1995.
[32] S. A. Ziai, B. Larijani, S. Akhoondzadeh, H. Fakhrzadeh, A. Dastpak, F. Bandarian, A. Rezai, H. N. Badi, and T. Emami, "Psyllium decreased serum glucose and glycosylated hemoglobin significantly in diabetic outpatients," J. Ethnopharmacol., vol. 102, pp. 202-207, 2005.
[33] B. H. Arjmandi, E. Sohn, S. Juma, S. R. Murthy, and B. P. Daggy, "Native and partially hydrolyzed psyllium have comparable effects on cholesterol metabolism in rats," Nutr., vol. 127, pp. 463-469, 1997.