Soybean and Fermented Soybean Extract Antioxidant Activities

Authors: W. Samruan, A. Oonsivilai, R. Oonsivilai

Abstract:

Today, people are more interested in the foods beneficial on their health. However, there are still lacks of accurate knowledge in the field of biological properties, functional properties, including the application of legume in foods. This study focused on antioxidant activity of soybean (SB) and fermented soybean (FSB) crude extracts evaluating to have more information in fortification SB and FSB crude extracts in food products and/or dietary supplement. SB and FSB crude extracts were prepared by infusion with water and ethanol. The antioxidant activity of crude extracts was studied with DPPH and ABTS assay including commercial standard. From both DPPH and ABTS assay, the antioxidant activity of SB and FSB water crude extract showed higher antioxidant activity than ethanol crude extract, and FSB crude extract showed higher antioxidant activity than SB crude extract. In DPPH assay, BHT and vitamin C showed IC50 values at 0.241, 0.039 mg/ml, in ABTS assay. In addition, Trolox showed IC50 at 0.058 mg/ml respectively. FSB water crude extract showed high antioxidant activity. Finally, the functional properties study of both water and ethanol crude extracts should be done for beneficial in application of these extracts in food products and dietary supplement in the near future.

Keywords: Antioxidant activity, Fermented soybean (FSB) crude extracts, soybean (SB) crude extracts.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1081719

References:

[1] Jing, L.G. and Zhang, Y. Z., 2006, Determination of Soybean Isoflavones Extracted from Soybean by HPLC, Journal of US-China Medical Science, 26(3): 629-632.
[2] Garcia, M. C., Torre, M., Marina, M. L., and Laborada, F.(1997).Composition and characterization of soybean and related products. Cri. Rev. Food Sci .Nutr.37:361-391.
[3] Messina, M., and Barns, S.(1991).The role of soy products in reduced risk of cancer. J. Natl. Cancer Inst.83:541-546.
[4] Hawrylewicz, E.J., Zapata, J.J., and Blair, W.H. (1995). Soy and experimental cancer: animal study.J.Nutr.125:698S-708S.
[5] Chiechia, L.M ., Secretob, G., Amorec, M D., Fanellid, M., Venturellib, E., Cantatorec, F., Valerioa, T ., Laselvac, G and Loizzia, P. (2002).Efficacy of a soy rich diet in preventing postmenopausal osteoporosis: the Menfis randomized trial .Maturitas.42: 295-300.
[6] Sumi, H., Hamada, H., Tsushima, H., Mihara, H., and Muraki, H.(1987). A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet. Experientia, 43: 1110-1111.
[7] Anthony, M.S., Carkson, T.B., Hughes, V.L., Jr. Morgan, T. M., and Burke, G.L.(1996).Soy isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkey.J.Nutr.126:43-50.
[8] Anderson, J.W., Smith, B.M, and Washnock, C.S. (1999). Cardiovascular and renal benefit of dry bean and soybean intake Am.J.Chin.Nutr.70:464s-474s.
[9] Brenner, B.M., Meyer, T.W., and Hastetter, T.H.(1982).Dietary protein b a d c 0.00 10.00 20.00 30.00 40.00 50.00 SBW FSBW SBE FSBE IC50 (mg/ml) Sample b a d c 0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 SBW FSBW SBE FSBE IC50(mg/ml) Sample intahe and the progressive nature of kidney disease: the role of hemodynamically mediated glomerular injury in the pathogenesis of progressive glomerular sclerosis in aging, renal ablation, and intrinsic renal disease..J.Med.307: 652-659.
[10] Kim, N.Y., Song, E.J., Kwon, D.Y., Kim, H.P., and Heo, M.Y.(2008).Antioxidant and antimutagenic activities of Korean fermented soybean. Food and chemical toxicology.46:1184-1189.
[11] Chukeatirote, E., and Thakang, P.(2006).Chemical composition of thua nao-a Fermented Soybean Food of Northern Thailand. Chaing Mai J.Sci.33(2):243-245.
[12] Chantawannakul, P., Oncharoen, A., Klanbut, K., Chukeatirote, E., and Lumyong, S. (2002).Characterization of protease of Bacillus subtilis strain 38 isolated from traditionally fermented soybean in Northern Thailand. ScienceAsia. 28 : 241-245.
[13] Moktan, B., Saha, J., and Sarkar, P.K. (2008). Antioxidant activities of soybean as affected by Bacillus-fermentation to kinema. Food Research International. 41: 586-593.
[14] Kwak, C.K., Lee, M.S., and Park, S.C.(2007). Higher antioxidant properties of Chungkookjang a fermented soybean paste, may be due to increased aglycone and malonylglycoside isoflavone during fermentation. Nutrition Research.27:719-727.
[15] Juan, M-Y., and Chou, C-C. (2010). Enhancement of antioxidant activity, total phenolic and flavonoid content of black soybeans by solid state fermentation with Bacillus subtilis BCRC14715 .Food Microbiology. 27:586-591.
[16] Dajanta, K., Apichartsrangkoon, A., Chukeatirote, E., Frazier,R.A. (2011). Free-amino acid profiles of thua nao, a Thai fermented soybean. Food Chemistry .125:342-347.
[17] Oonsivilai,R., Cheng, C.,Bomser, J.A., Ferruzzi, M.G., and Ningsanond, S.(2007).Phytochemical profiling and detoxification properties of Thunbergia Laurifolia Lindl (Rang Chuet) extracts.Journal of Ethnopharmacology. 114:300-306.
[18] Ksouri, R., Falleh, H., Megdiche ,W., Trabelsi, N., Mhamdi, B., Chaieb, K., Bakrouf, A., Magné, C., and Abdelly, C.(2009). Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L. and related polyphenolic constituents. Food and Chemical Toxicology 47: 2083-2091.
[19] J. Singthong, R. Oonsivilai, J. Oonmetta-aree, S. Ningsanond. 2012. Phytochemical profiles, antioxidant activity, and cytotoxicity of Cissampelos pareira (Krueo Ma Noy) extract on Caco-2 cells. The 14th Food Innovation Asia Conference 2012. Bitec, Bagna, Bangkok, June 14-15, Poster Presentation.
[20] Oonsivilai, R., Singthong, J. Oonmetta-aree, J., and Ningsanond, S. 2012. Bioactivity, antioxidant activity, and cytotoxicity of Yanang, Kreu-Ma Noy, and Rang Chuet extracts. IFT Annual Meeting 2012, Las Vegas, USA, June 23-29, Poster Presentation.
[21] R. Oonsivilai, N. Chaijareonudomroung, Y. Huantanom., and A. Oonsivilai. 2010. Extraction condition of Echinocactus grusonii. World Academy of Science, Engineering and Technology Journal. 70: 366: 369.
[22] Singthong, J. Oonsivilai, R., Oonmetta-aree., and Ningsanond, S. 2011. Phytochemical oprofile, antioxidant activity and cytotoxicity of Tiliacora triandra (Colebr.) Diels. (Yanang) extracts on Caco-2-cells. The 5th Thailand Congress of Nutrition 2011. September 5-7. Oral Presentation.