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Production of Apricot Vinegar Using an Isolated Acetobacter Strain from Iranian Apricot
Abstract:Vinegar or sour wine is a product of alcoholic and subsequent acetous fermentation of sugary precursors derived from several fruits or starchy substrates. This delicious food additive and supplement contains not less than 4 grams of acetic acid in 100 cubic centimeters at 20°C. Among the large number of bacteria that are able to produce acetic acid, only few genera are used in vinegar industry most significant of which are Acetobacter and Gluconobacter. In this research we isolated and identified an Acetobacter strain from Iranian apricot, a very delicious and sensitive summer fruit to decay, we gathered from fruit's stores in Isfahan, Iran. The main culture media we used were Carr, GYC, Frateur and an industrial medium for vinegar production. We isolated this strain using a novel miniature fermentor we made at Pars Yeema Biotechnologists Co., Isfahan Science and Technology Town (ISTT), Isfahan, Iran. The microscopic examinations of isolated strain from Iranian apricot showed gram negative rods to cocobacilli. Their catalase reaction was positive and oxidase reaction was negative and could ferment ethanol to acetic acid. Also it showed an acceptable growth in 5%, 7% and 9% ethanol concentrations at 30°C using modified Carr media after 24, 48 and 96 hours incubation respectively. According to its tolerance against high concentrations of ethanol after four days incubation and its high acetic acid production, 8.53%, after 144 hours, this strain could be considered as a suitable industrial strain for a production of a new type of vinegar, apricot vinegar, with a new and delicious taste. In conclusion this is the first report of isolation and identification of an Acetobacter strain from Iranian apricot with a very good tolerance against high ethanol concentrations as well as high acetic acid productivity in an acceptable incubation period of time industrially. This strain could be used in vinegar industry to convert apricot spoilage to a beneficiary product and mentioned characteristics have made it as an amenable strain in food and agricultural biotechnology.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1080223Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF
 Ndoye, B., L. Cleenwerck, K. Engelbeen, R. Dubois-Dauphin, A.T. Guiro, S.V. Trappen, A. Willems and P. Thonart, 2007. Acetobacter senegalensis sp. nov., a thermotolerant acetic acid bacterium isolated in Senegal (sub-Saharan Africa) from mango fruit (Mangifera indica L.). Int. J. Syst. Evol. Microbiol., 57: 1576-1581.
 Beheshti Maal, K. and R. Shafiei, 2010. Isolation and characterization of an Acetobacter strain from Iranian white-red cherry as a potential strain for cherry vinegar production in microbial biotechnology. Asian J. Biotechnol., 1: 53-59.
 Gullo M. and P. Giudici, 2008. Acetic acid in traditional balsamic vinegar, phenotypic traits relevant for starter cultures selection. Int. J. Food Microbiol., 125: 46-53.
 Beheshti Maal, K. and R. Shafiei, 2012. Characterization of an Acetobacter strain isolated from Iranian peach that tolerates high temperatures and ethanol concentrations. Int. J. Biol. Life. Sci. , 4: 239- 243.
 Kadere T.T., T. Miamoto, R.K. Oniang-o, P.M. Kutima and S.M. Njoroge, 2008. Isolation and identification of genera Acetobacter and Gluconobacter in coconut toddy (mnazi). Afr. J. Biotechnol., 7: 2963- 2971.
 Kocher G.S., K.L. Kalra and R.P. Phutela, 2006. Comparative production of sugarcane vinegar by different immobilization techniques. J. Inst. Brew., 112: 264-266.
 Drydale, G.S. and G. H. Fleet, 1985. Acetic acid bacteria in some Australian wines. Food Technol. Aust., 37: 17-20.
 Joyeux A., S. Lafon-Lafourcade and P. Ribereau-Gayon P, 1984. Evolution of acetic acid bacteria during fermentation and storage of wine. Appl. Environ. Microbiol., 48: 153-156.
 Beheshti Maal, K. and R. Shafiei, 2011. A thermotolerant Acetobacter strain isolated from Iranian peach suitable for industrial microbiology. Asian J. Biol. Sci., 3: 244-251.
 Faparusi S.I., 1973. Origin of initial microflora of palm wine from oil palm trees (Elaeis guineensis). J. Appl. Bacteriol., 36: 559-565.
 Sossou, S.K., Y. Ameyapoh, S.D. Karou and C.D. Souza, 2009. Study of pineapple peelings processing into vinegar by biotechnology. Pak. J. Biol. Sci., 11: 859-865.
 Moryadee, A. and W. Pathon-Aree, 2008. Isolation of thermotolerant acetic acid bacteria from fruits for vinegar production. Res. J. Microbiol., 3: 209-212.
 Nanda K., M. Taniguchi, S. Ujike, N. Ishihara, H. Mori, H. Ono and Y. Murooka, 2001. Characterization of acetic acid bacteria in traditional acetic acid fermentation of rice vinegar (komesu) and unpolished rice vinegar (kurosu) produced in Japan. Appl. Environ. Microbiol., 67: 986- 990.
 IIha, E.C., E.S. Anna, R.C. Torres, A.C. Porto and E.M. Meinert, 2000. Utilization of bee (Apis mellifera) honey for vinegar production at laboratory scale. Acta Cie. Ven., 51: 231-235.
 Falcone P. M. and P. Giudici, 2008. Molecular size and molecular size distribution affecting traditional balsamic vinegar aging. J. Agri. Food Chem., 56: 7057-7066.